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Samenvatting Moleculaire diagnostiek blok 1 minor Medische Microbiologie

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Uploaded on
14-10-2022
Written in
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In deze samenvatting komen verschillende moleculaire diagnostiek methodes aanbod die belangrijk zijn in de Medische Microbiologie (en voor het kennistentamen van de minor blok 1). Daarnaast worden de technieken uitgelegd en komen er plaatjes aan bod waardoor het verhaal wordt ondersteund.

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Institution
Hogeschool Arnhem En Nijmegen (HAN)
Course
Minor Medische Microbiologie Mol Diagnostiek










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Institution
Hogeschool Arnhem en Nijmegen (HAN)
Study
Biologie En Medisch Laboratoriumonderzoek
Course
Minor Medische Microbiologie Mol Diagnostiek
All documents for this subject (1)

Document information

Uploaded on
October 14, 2022
Number of pages
16
Written in
2022/2023
Type
Summary

Subjects

  • gebruik van plaatjes
  • college aantekeningen
  • sanger sequencing
  • eiwitdetectie
  • genoomdetectie
  • nucleïnezuuranalyse
  • nederlandstalige samenvatting
  • samenvatting
  • next generation sequencing
  • antigeenantilichaam

Content preview

Moleculaire diagnostiek samenvatting minor
Medische Microbiologie blok 1




Made by: Iris Gülcher

,Inhoudsopgave
Moleculaire diagnostiek les 1: ............................................................................................................. 3
Moleculaire diagnostiek les 2 t/m 5 presentaties ............................................................................... 9
Moleculaire diagnostiek les 6: ........................................................................................................... 10
Moleculaire diagnostiek les 7: ........................................................................................................... 12




Made by: Iris Gülcher

, Moleculaire diagnostiek les 1:
-Moleculaire diagnostieken:
>Analyseren moleculen DNA/RNA/eiwitten en/of antilichamen
>Pathogenen
>Afwijkingen in genetisch materiaal
-Introductie moleculaire diagnostieken:
>




-Isolatie pathogeen:
>Microscopie
>Cultuur
>Voordelen en nadelen
-Antigeen/antilichaam detectie:
>Serologie/immunologie
>Antigeen-antilichaam
>Interferon-y
>Voordelen/nadelen
-Eiwit detectie:
>MALDI-TOF
>Voordelen/nadelen
-Genoom detectie:
>PCR
>RRT-PCR
>Voordelen/nadelen
-Diagnostische testen:
>Belangrijke aspecten:
*Specificiteit (specifiek zijn voor wat je wilt aantonen)
*Gevoeligheid
*Looptijd (niet te lang duren)
*Training van personeel (niet te moeilijk qua training)
*Apparatuur/lab (wel of niet op het lab)
*Kosten
*Betrouwbaarheid
-Nucleïnezuuranalyse:
>Nucleïnezuur isolatie (en daarna vermeerderen)
>PCR, sequencing
>RNA -> omkeerbare transcriptase
>Geautomatiseerde NA isolatie
*Voorbeeld: Roche MagnaPure
>UV-spectrometrie:
*Kwantiteit en kwaliteit
>Gelelektroforese
>Amplificatie: PCR, sequencing (wordt het meeste gebruikt)
Made by: Iris Gülcher
$6.04
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iris70 Hogeschool Arnhem en Nijmegen
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