Applications of Cell and
Tissue Culture
By
HASNAT MUEEN
, Applications of cell and tissue culture
Table of Contents
1 Applications of Plant Cell and Tissue Culture: .................................................................. 1
1.1 Haploids and Double haploids: ................................................................................... 1
1.1.1 Production of haploids and double haploids: ....................................................... 1
1.1.2 Factors Affecting Haploid Induction: .................................................................. 2
1.1.3 Uses: ..................................................................................................................... 3
1.2 Micropropagation: ....................................................................................................... 3
1.2.1 Technique of Micro propagation: ........................................................................ 3
1.2.2 Uses: ..................................................................................................................... 4
1.3 Somatic embryogenesis: .............................................................................................. 5
1.3.1 Production of somatic embryos: .......................................................................... 5
1.3.2 Uses ...................................................................................................................... 5
1.4 Synthetic seed technology: .......................................................................................... 6
1.4.1 Uses: ..................................................................................................................... 6
1.5 Somaclonal variations: ................................................................................................ 7
1.5.1 Production of somaclonal variations:................................................................... 7
1.5.2 Uses: ..................................................................................................................... 7
1.6 Germplasm conservation:............................................................................................ 8
1.6.1 In vivo gene banks: .............................................................................................. 8
1.6.2 In vitro gene banks: .............................................................................................. 8
1.6.3 Cold storage: ........................................................................................................ 8
1.6.4 Cryopreservation: ................................................................................................. 9
1.6.5 Uses of Germplasm Conservation: ...................................................................... 9
1.7 Secondary metabolites production: ............................................................................. 9
1.7.1 Requirements for production of plant cultures to get secondary metabolites:..... 9
1.7.2 Important secondary metabolites: ...................................................................... 10
1.8 Biotransformation: .................................................................................................... 10
1.9 Somatic hybrids and cybrids: .................................................................................... 11
1.9.1 Process of somatic hybridization: ...................................................................... 11
1.9.2 Cybrids and Cybridization: ................................................................................ 11
1.9.3 Applications of somatic hybridization: .............................................................. 12
1.9.4 Applications of Cybrids: .................................................................................... 12
, Applications of cell and tissue culture
1.10 Transgenic methods and transgenic plants: ............................................................... 12
2 Applications of Animal Cell and Tissue Culture: ............................................................ 13
2.1 Cell culture-based vaccines: ...................................................................................... 13
2.1.1 Vaccine production: ........................................................................................... 13
2.1.2 Uses and importance: ......................................................................................... 14
2.2 Organ culture: ............................................................................................................ 14
2.2.1 Organ culture methods: ...................................................................................... 14
2.2.2 Uses: ................................................................................................................... 16
2.3 Reproductive cloning: ............................................................................................... 16
2.3.1 Cloning using somatic nuclear transfer: ............................................................ 16
2.3.2 Uses: ................................................................................................................... 17
2.4 Monoclonal antibodies: ............................................................................................. 17
2.4.1 Production: ......................................................................................................... 17
2.4.2 Uses: ................................................................................................................... 18
2.5 Somatic cell fusion: ................................................................................................... 18
2.5.1 Procedure: .......................................................................................................... 18
2.5.2 Uses: ................................................................................................................... 19
2.6 Production of t-PA: ................................................................................................... 19
2.6.1 Uses: ................................................................................................................... 19
2.7 Production of factor VIII:.......................................................................................... 19
2.7.1 Uses: ................................................................................................................... 20
2.8 Stem cell culture: ....................................................................................................... 20
2.8.1 Uses: ................................................................................................................... 21
2.9 Cell and tissue engineering: ...................................................................................... 22
2.9.1 Requirements of tissue construct: ...................................................................... 22
2.9.2 Uses: ................................................................................................................... 23
2.10 Other miscellaneous applications: ............................................................................. 23
Tissue Culture
By
HASNAT MUEEN
, Applications of cell and tissue culture
Table of Contents
1 Applications of Plant Cell and Tissue Culture: .................................................................. 1
1.1 Haploids and Double haploids: ................................................................................... 1
1.1.1 Production of haploids and double haploids: ....................................................... 1
1.1.2 Factors Affecting Haploid Induction: .................................................................. 2
1.1.3 Uses: ..................................................................................................................... 3
1.2 Micropropagation: ....................................................................................................... 3
1.2.1 Technique of Micro propagation: ........................................................................ 3
1.2.2 Uses: ..................................................................................................................... 4
1.3 Somatic embryogenesis: .............................................................................................. 5
1.3.1 Production of somatic embryos: .......................................................................... 5
1.3.2 Uses ...................................................................................................................... 5
1.4 Synthetic seed technology: .......................................................................................... 6
1.4.1 Uses: ..................................................................................................................... 6
1.5 Somaclonal variations: ................................................................................................ 7
1.5.1 Production of somaclonal variations:................................................................... 7
1.5.2 Uses: ..................................................................................................................... 7
1.6 Germplasm conservation:............................................................................................ 8
1.6.1 In vivo gene banks: .............................................................................................. 8
1.6.2 In vitro gene banks: .............................................................................................. 8
1.6.3 Cold storage: ........................................................................................................ 8
1.6.4 Cryopreservation: ................................................................................................. 9
1.6.5 Uses of Germplasm Conservation: ...................................................................... 9
1.7 Secondary metabolites production: ............................................................................. 9
1.7.1 Requirements for production of plant cultures to get secondary metabolites:..... 9
1.7.2 Important secondary metabolites: ...................................................................... 10
1.8 Biotransformation: .................................................................................................... 10
1.9 Somatic hybrids and cybrids: .................................................................................... 11
1.9.1 Process of somatic hybridization: ...................................................................... 11
1.9.2 Cybrids and Cybridization: ................................................................................ 11
1.9.3 Applications of somatic hybridization: .............................................................. 12
1.9.4 Applications of Cybrids: .................................................................................... 12
, Applications of cell and tissue culture
1.10 Transgenic methods and transgenic plants: ............................................................... 12
2 Applications of Animal Cell and Tissue Culture: ............................................................ 13
2.1 Cell culture-based vaccines: ...................................................................................... 13
2.1.1 Vaccine production: ........................................................................................... 13
2.1.2 Uses and importance: ......................................................................................... 14
2.2 Organ culture: ............................................................................................................ 14
2.2.1 Organ culture methods: ...................................................................................... 14
2.2.2 Uses: ................................................................................................................... 16
2.3 Reproductive cloning: ............................................................................................... 16
2.3.1 Cloning using somatic nuclear transfer: ............................................................ 16
2.3.2 Uses: ................................................................................................................... 17
2.4 Monoclonal antibodies: ............................................................................................. 17
2.4.1 Production: ......................................................................................................... 17
2.4.2 Uses: ................................................................................................................... 18
2.5 Somatic cell fusion: ................................................................................................... 18
2.5.1 Procedure: .......................................................................................................... 18
2.5.2 Uses: ................................................................................................................... 19
2.6 Production of t-PA: ................................................................................................... 19
2.6.1 Uses: ................................................................................................................... 19
2.7 Production of factor VIII:.......................................................................................... 19
2.7.1 Uses: ................................................................................................................... 20
2.8 Stem cell culture: ....................................................................................................... 20
2.8.1 Uses: ................................................................................................................... 21
2.9 Cell and tissue engineering: ...................................................................................... 22
2.9.1 Requirements of tissue construct: ...................................................................... 22
2.9.2 Uses: ................................................................................................................... 23
2.10 Other miscellaneous applications: ............................................................................. 23
