PURINES
Outline
1. De novo and salvage pathways for purine nucleotides biosynthesis
2. Regulation of de novo biosynthesis of purine nucleotides
3. Degradation of purine nucleotides
4. Clinical significance of purine nucleotides metabolism:
1
, ✓ Gout, drugs used in treatment of gout
✓ Severe combined Immunodeficiency (SCID)
✓ Lesch-Nyhan syndrome
5. Antimetabolites of purines nucleotides biosynthesis in cancer
therapy;
✓ 6- Mercaptopurine,
✓ Azaserine,
✓ Folic acid analogs
2
,Nucleoside and Nucleotide
3
, Roles of nucleotides in cell physiology
i. Precursors for deoxyribonucleic acid (DNA) and
ribonucleic acid (RNA) synthesis
ii. Components of coenzymes such as NAD(H),
NADP(H), FMN(H2), Coenzyme A iii.
Source of energy driving many metabolic
processes e.g ATP, GTP
iv. Carriers in biosynthesis e,g UDP for carbohydrates
and CTP for lipids (UDP-glucose, CDP-diacylglycerol)
4
Outline
1. De novo and salvage pathways for purine nucleotides biosynthesis
2. Regulation of de novo biosynthesis of purine nucleotides
3. Degradation of purine nucleotides
4. Clinical significance of purine nucleotides metabolism:
1
, ✓ Gout, drugs used in treatment of gout
✓ Severe combined Immunodeficiency (SCID)
✓ Lesch-Nyhan syndrome
5. Antimetabolites of purines nucleotides biosynthesis in cancer
therapy;
✓ 6- Mercaptopurine,
✓ Azaserine,
✓ Folic acid analogs
2
,Nucleoside and Nucleotide
3
, Roles of nucleotides in cell physiology
i. Precursors for deoxyribonucleic acid (DNA) and
ribonucleic acid (RNA) synthesis
ii. Components of coenzymes such as NAD(H),
NADP(H), FMN(H2), Coenzyme A iii.
Source of energy driving many metabolic
processes e.g ATP, GTP
iv. Carriers in biosynthesis e,g UDP for carbohydrates
and CTP for lipids (UDP-glucose, CDP-diacylglycerol)
4
