Portage Learning
BIO 171- Microbiology
Lab Notebook
Lab 1 Notebook
Lab 2 Notebook
Lab 3 Notebook
Lab 4 Notebook
Lab 5 Notebook
Lab 6 Notebook
Lab 7 Notebook
Lab 8 Notebook
Lab 9 Notebook
, Lab 1 Notebook
Title: 01 Introduction to Medical Microbiology
Objective:
Cultivation of lab samples (growth conditions) that are necessary for carrying out experiments
and study pathogens in a lab setting.
Procedure:
• Equipment Used:
o Keep a sterile environment to keep people healthy
o The tool for this is the Autoclave.
▪ Cleans equipment at 125⁰C
o Growing: Fixed incubator or a shaker incubator
▪ Fixed incubator operates at 37 C
▪ Shaker
▪ Visualization: Microscopy
▪ Storage: A refrigerator at 4 C
o General Lab Safety:
▪ Never eat or drink in the lab
• Contamination risks
▪ Use personal protective equipment (PPE)
, • Latex, nitrile (non-latex gloves) thermal, thermal cold
gloves (application dependent)
• Lab coat
▪ Never leave the lab while wearing PPE always remove before leaving.
• Public hallway
• Bathroom
• Cafeteria
o Lab Notebook: Required for the course
▪ Titling the experiment: First, Last initial exp# and Title of the experiment.
• Ex: OH01 Keeping a Lab Notebook
▪ Objective: The focus of the experiment
• Ex: Objective: To establish an organized template for
keeping experimental records, procedures, and results.
▪ Procedure: list the steps to any procedure in the lab.
• This is where each step of a given protocol is recorded.
• Be sure to clearly indicate any deviations that may occur
during the experiment.
▪ Notes: Any additional information placed in this section.
▪ Results: A summary of the outcome of the experiment should
be described here.
Identification of samples (biochemical assays) to determine what kind of bacteria or pathogen a
patient contracted.
• Tests available for each
pathogen: Evaluation of samples
(microscopy)
• Visualization and recognition of key characteristics of pathogens:
, Notes:
Equipment Used:
o Keep a sterile environment to keep people healthy
o The tool for this is the Autoclave.
▪ Cleans equipment at 125⁰C
▪ Uses heat pressure and steam to sterilize your environment.
▪ A chamber with a thick door that will be closed with equipment or
a sample inside to sterilize.
▪ Uses hot steam instead of hot air because it sterilizes in a matter
of minutes verses several hours.
▪ When opening the chamber after a cycle is complete, be sure to open
slowly and carefully after it has depressurized to avoid being burned
by the hot steam that will be released.
o Growing: Fixed incubator or a shaker incubator
▪ Fixed incubator operates at 37 C that houses petri dishes, which
holds samples, and tubes which hold liquid cultures. It has a door
that seals such to maintain dark conditions for growing.
▪ Shaker: growth media, a liquid nutrient broth, is placed in the metal
holders to be shook. The shaker is used to inoculate bacteria as it
rotates the sample to aerate it and provide optimal conditions for
bacteria growth.
▪ Visualization: Microscopy
▪ Storage: A refrigerator at 4O C is used to slow the growth of bacteria
and preserve samples. Petri dishes are wrapped in a para film, a waxy
film to keep it airtight.
o General Lab Safety:
▪ Use personal protective equipment (PPE)
• non-latex available usually purple, blue, or green
BIO 171- Microbiology
Lab Notebook
Lab 1 Notebook
Lab 2 Notebook
Lab 3 Notebook
Lab 4 Notebook
Lab 5 Notebook
Lab 6 Notebook
Lab 7 Notebook
Lab 8 Notebook
Lab 9 Notebook
, Lab 1 Notebook
Title: 01 Introduction to Medical Microbiology
Objective:
Cultivation of lab samples (growth conditions) that are necessary for carrying out experiments
and study pathogens in a lab setting.
Procedure:
• Equipment Used:
o Keep a sterile environment to keep people healthy
o The tool for this is the Autoclave.
▪ Cleans equipment at 125⁰C
o Growing: Fixed incubator or a shaker incubator
▪ Fixed incubator operates at 37 C
▪ Shaker
▪ Visualization: Microscopy
▪ Storage: A refrigerator at 4 C
o General Lab Safety:
▪ Never eat or drink in the lab
• Contamination risks
▪ Use personal protective equipment (PPE)
, • Latex, nitrile (non-latex gloves) thermal, thermal cold
gloves (application dependent)
• Lab coat
▪ Never leave the lab while wearing PPE always remove before leaving.
• Public hallway
• Bathroom
• Cafeteria
o Lab Notebook: Required for the course
▪ Titling the experiment: First, Last initial exp# and Title of the experiment.
• Ex: OH01 Keeping a Lab Notebook
▪ Objective: The focus of the experiment
• Ex: Objective: To establish an organized template for
keeping experimental records, procedures, and results.
▪ Procedure: list the steps to any procedure in the lab.
• This is where each step of a given protocol is recorded.
• Be sure to clearly indicate any deviations that may occur
during the experiment.
▪ Notes: Any additional information placed in this section.
▪ Results: A summary of the outcome of the experiment should
be described here.
Identification of samples (biochemical assays) to determine what kind of bacteria or pathogen a
patient contracted.
• Tests available for each
pathogen: Evaluation of samples
(microscopy)
• Visualization and recognition of key characteristics of pathogens:
, Notes:
Equipment Used:
o Keep a sterile environment to keep people healthy
o The tool for this is the Autoclave.
▪ Cleans equipment at 125⁰C
▪ Uses heat pressure and steam to sterilize your environment.
▪ A chamber with a thick door that will be closed with equipment or
a sample inside to sterilize.
▪ Uses hot steam instead of hot air because it sterilizes in a matter
of minutes verses several hours.
▪ When opening the chamber after a cycle is complete, be sure to open
slowly and carefully after it has depressurized to avoid being burned
by the hot steam that will be released.
o Growing: Fixed incubator or a shaker incubator
▪ Fixed incubator operates at 37 C that houses petri dishes, which
holds samples, and tubes which hold liquid cultures. It has a door
that seals such to maintain dark conditions for growing.
▪ Shaker: growth media, a liquid nutrient broth, is placed in the metal
holders to be shook. The shaker is used to inoculate bacteria as it
rotates the sample to aerate it and provide optimal conditions for
bacteria growth.
▪ Visualization: Microscopy
▪ Storage: A refrigerator at 4O C is used to slow the growth of bacteria
and preserve samples. Petri dishes are wrapped in a para film, a waxy
film to keep it airtight.
o General Lab Safety:
▪ Use personal protective equipment (PPE)
• non-latex available usually purple, blue, or green
