studying cells
,Magnification
• Object –
sample under
microscope
,Resolution
• Minimum distance apart 2 objects can be to appear as separate
• Light – 0.2um
• TEM – 0.1nm
• SEM – 10nm
• Up until max resolution, increasing magnification shows detail but
then it is blurry
, Cell fractionation
• Cells are broken up and organelles are separated
• Before fractionation, cells are in a cold, buffered, isotonic solution
• Cold – reduce enzyme activity so organelles are whole
• Buffered – stops pH changes
• Isotonic – prevent bursting/shrinking due to osmosis