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Lecture notes Biology (SBIB011)

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BIOLOGY NOTES ABOUT EVERYTHING YOU MIGHT NEED TO KNOW REGARDING MICROSCOPES. ALSO CONSIST OF FEW PRACTISE QUESTIONS.

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Uploaded on
May 3, 2022
Number of pages
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Written in
2021/2022
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Ms malakane
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Introduction to microscope

,•
Invention of
Over time, our knowledge of life has increased &
continues to increase
microscopes
• The invention of Light microscope was a big step –
can magnify objects up to 500 times

• Later, Electron microscope was invented – magnifies
objects >100 000 times

• Electron microscope has powerful resolving power
which can reveal details of specimens

, Microscopy
Scientists use microscopes to visualize cells too small
to see with the naked eye
In a light microscope (LM), visible light is passed
through a specimen and then through glass lenses
Lenses refract (bend) the light, so that the image is
magnified
Magnification allows the image of a microscopic object
to be seen
Resolving power- the ability to distinguish between two
point close together
Contrast- ability to distinguish an object/ part there of
against a background
Object = specimen
Image = what you see when various lenses are used to
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