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BIOS 242 Week 6 iLab: Specific Immunity
SPECIFIC IMMUNITY
Introduction
This lab experiment was done to see if there were any pathogens in the watermelon that a
student ate after it falling in the barn. My case study involved students being at a barn and
everyone enjoying and playing games. One of the students dropped his slice of
watermelon in the barn. He picked it up, and simply just rinsed it off and proceeded to eat
it. The sponsor knew the watermelon would be harmful, so took the slice and put it in a
plastic bag and taken to the lab. The results of that stain were that it had gram negative
rods.
Procedure
You will conduct four biochemical tests (Acid from Glucose, Gas from Glucose, Lactose
Fermentation, Indole Production, Lysine decarboxylase, and Malonate Utilization) on
your unknown bacterium sample. You may have noted that you have conducted several
of these tests in previous weeks.
Conduct Acid from Glucose and Gas from Glucose Tests:
1. Click the New Unknown button and type SpecificImmunity in the “Enter a Label”
field. Select Specific Immunity from the Subgroup dropdown menu.
2. Click the box allowing autoinoculation. Click OK.
3. Record your case study. iLab: Specific Immunity Page 3
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4. Record the results of the Gram Stain.
5. Open the Acid from Glucose Test from the Biochemical Tests Reference Book.
Review the steps of this test and determination of test results. NOTE: You have
conducted both of these tests in a previous week’s experiment.
6. Select Phenol Red Glucose Broth Durham Tube as your medium. Label the medium:
PhenolRedGlucose. Click OK.
7. Inoculate your medium. Place in 37 degree incubator. Click Next Day.
8. Retrieve sample from incubator. Observe and record the results for Acid from Glucose
and Gas from Glucose tests.
Conduct Lactose Fermentation Test:
1. Open the Lactose Fermentation Test from the Biochemical Tests Reference book.
Review the steps of the test and determination of test results. NOTE: You have conducted
both of these tests in a previous week’s experiment.
2. From the Media dropdown menu, select Phenol Red Lactose Broth.
3. Enter PhenolRedLactose in the Medium Label window to label your sample. Click
OK.
4. Inoculate your medium. Place in 37 degree incubator. Click Next Day.
5. Retrieve sample from incubator. Observe and record results.
Conduct Lactose Fermentation Test:
BIOS 242 Week 6 iLab: Specific Immunity
SPECIFIC IMMUNITY
Introduction
This lab experiment was done to see if there were any pathogens in the watermelon that a
student ate after it falling in the barn. My case study involved students being at a barn and
everyone enjoying and playing games. One of the students dropped his slice of
watermelon in the barn. He picked it up, and simply just rinsed it off and proceeded to eat
it. The sponsor knew the watermelon would be harmful, so took the slice and put it in a
plastic bag and taken to the lab. The results of that stain were that it had gram negative
rods.
Procedure
You will conduct four biochemical tests (Acid from Glucose, Gas from Glucose, Lactose
Fermentation, Indole Production, Lysine decarboxylase, and Malonate Utilization) on
your unknown bacterium sample. You may have noted that you have conducted several
of these tests in previous weeks.
Conduct Acid from Glucose and Gas from Glucose Tests:
1. Click the New Unknown button and type SpecificImmunity in the “Enter a Label”
field. Select Specific Immunity from the Subgroup dropdown menu.
2. Click the box allowing autoinoculation. Click OK.
3. Record your case study. iLab: Specific Immunity Page 3
, 2
4. Record the results of the Gram Stain.
5. Open the Acid from Glucose Test from the Biochemical Tests Reference Book.
Review the steps of this test and determination of test results. NOTE: You have
conducted both of these tests in a previous week’s experiment.
6. Select Phenol Red Glucose Broth Durham Tube as your medium. Label the medium:
PhenolRedGlucose. Click OK.
7. Inoculate your medium. Place in 37 degree incubator. Click Next Day.
8. Retrieve sample from incubator. Observe and record the results for Acid from Glucose
and Gas from Glucose tests.
Conduct Lactose Fermentation Test:
1. Open the Lactose Fermentation Test from the Biochemical Tests Reference book.
Review the steps of the test and determination of test results. NOTE: You have conducted
both of these tests in a previous week’s experiment.
2. From the Media dropdown menu, select Phenol Red Lactose Broth.
3. Enter PhenolRedLactose in the Medium Label window to label your sample. Click
OK.
4. Inoculate your medium. Place in 37 degree incubator. Click Next Day.
5. Retrieve sample from incubator. Observe and record results.
Conduct Lactose Fermentation Test:
