BIOD 171 AM04 Intro to Growth Media

BIOD 171 AM04 Intro to Growth MediaAM04 Intro to Growth Media Objective: To understand the types and uses of growth media for the isolation and identification of unknown bacterial samples. Notes: Non-selective media  Non-selective media: important for the expansion of unknown bacteria o Anything will grow on media plates o Provides carbon source  LB: o Most common nutrient agar o Light yellow in color o Place bacteria on the plate, invert it (turn upside down) and store at 37  Invert it so nothing settles on top of it  Blood Agar: o Contains red blood cells o Important as a nutrient source o Tryptic soy agar yeast + red blood cells  TSAYE: o Tryptic soy agar yeast extract o If you remove the red blood cells o Nutrients: tryptic soy and yeast Selective media  Selective media: used to eliminate irrelevant bacteria from mixed cultures  MacConkey: o Slight cloudiness o Red color