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Uitwerking practicum DNA BGZ2022

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In dit document vind je de uitwerking van het uiteindelijke rapport van het DNA practicum binnen het BGZ2022 blok. Dit omvat de resultaten van het practicum, maar ook de voorbereidende vragen die voorafgaand aan het practicum dienden worden te ingeleverd.

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Uploaded on
November 15, 2021
Number of pages
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Written in
2021/2022
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Practicum vragen
Lisa Willems i6198870

DR na
BGZ2222

Academisch jaar: 2020–2021




© AmoebaSisters




Faculty of Health, Medicine and Life Sciences
Bachelor Gezondheidswetenschappen


 2020 Maastricht University, Faculty of Health, Medicine and Life Sciences
Niets uit deze uitgave mag verveelvoudigd en/of openbaar gemaakt worden door middel van druk, offset,
fotokopie of in enige digitale, elektronische of andere vorm zonder voorafgaande schriftelijke toestemming van
de eigenaar van het auteursrecht.

, Faculty of Health, Medicine and Life Sciences




Inhoudsopgave

Aftekening-rapport.............................................................................................................................3
DNA (vragen 1A-I)...........................................................................................................................4
PCR (vragen 2A-3D)........................................................................................................................6
Restrictie analyse (vragen 4A-F).....................................................................................................9
DNA zuiverheid en kwantificering (vragen 5A-E)..........................................................................14
Gel electroforese (vragen 6A-C):..................................................................................................14
Opdracht Resultaten DNA spectrofotometrie:.................................................................................15
Opdracht Resultaten PCR:................................................................................................................15
Opdracht Resultaten restrictie-analyse:...........................................................................................17




BGZ2222 2020-2021 2

, Faculty of Health, Medicine and Life Sciences




Aftekening-rapport

 Antwoorden op de vragen gemaakt voorafgaand aan ieder practicum dienen in
dit formulier te worden ingevuld en aan het eind van het blok te worden ge-
upload.
 Verder dient dit rapport te bevatten:
o Een uitdraai/foto van de DNA spectrofotometrie met uitleg en berekening
DNA opbrengst
o Een foto van de PCR gel+ uitleg
o Een foto van de restrictie analyse+uitleg
 Dit formulier dient aan het eind van week 6 digitaal te worden aangeleverd via de
assignment site van CANVAS: deadline xxxx 23.59 uur.




BGZ2222 2020-2021 3
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