Genetics Unit 6 Exam ALL ANSWERS SOLUTION AID 100% CORRECT ANSWERS GUARANTEE GRADE A+

A major disadvantage with current gene therapy techniques using viral vectors is the possibility of an immune response against the virus. Selected Answer: True • Question 2 Answer the following questions based on the figure below. Figure 1 shows the products from Sanger sequencing. If a primer is designed to hybridize to the template strand for PCR amplification, what will its last four bases be (5' to 3', left to right)? Selected Answer: d. CGCT • Question 3 Use the BLAST tool at CCKHPEAKRM PCAEDYLSVV LNQLCVLHEK TPVSDRVTKC CTESLVNRRP Paste this sequence into the “Enter Query Sequence” window, scroll to the bottom of the page, and click on “BLAST”. What is the identity of the protein? Selected Answer: c. albumin • Question 4 A plasmid has tetR and lacZ genes for tetracycline resistance and ß-galactosidase, respectively. There is a single EcoRI site within lacZ, and a single Hind III site within tetR. The EcoRI-HindIII cut plasmid is combined with a DNA fragment with compatible ends. Cells that contain the plasmid with the insert will be Selected Answer: d. tetracycline sensitive and white if grown on X-gal. • Question 5 What does physical mapping provide that the other types of genetic mapping do not? Selected Answer: b. Number of nucleotide base-pairs between genes • Question 6 When bacterial transformants containing a library are transferred to nitrocellulose filters and screened, one of the steps is treatment with an alkaline solution. What does this do? Selected Answer: e. lyses the cells and denatures the released DNA • Question 7 Using the NeoR/Tk system of introducing gene replacements into mice (as described in the text), which of the following would identify those mouse cells that were the result of homologous recombination? Selected Answer: a. NeoR only • Question 8 The genomic equivalent of a library is determined by multiplying the number of clones on a plate by the size of the average insert. Selected Answer: False • Question 9 Which of the following is an example of a recombinant DNA molecule? Selected Answer: b. a genomic fragment of mouse DNA ligated to