PLASMA SERUM • Materials required but not supplied
• Liquid portion of an unclotted blood • Liquid portion of clotted blood o Current laboratory instrumentation. Spectrophotometer U
• Contains the fibrinogen group of • Does not contain the fibrinogen group thermostatic cuvette holder. Automatic micropipettes. Glass o
coagulation factors of coagulation factors polystyrene cuvettes. Saline solution.
• Lighter yellow and a little turbid • Darker yellow and clearer • Reagent preparation
• Chemically, does not contain much of • Contains higher levels of platelet o Use reagent ready to use
platelet derivatives. derivatives o Stability: Up to expiration date on labels at 2 to 8º C
o Stability since first opening of vials: Preferably 60 days at 2 to 8
Characteristics of Blood: • Specimen
• Physical o Serum, Plasma, Urine, CSF
- pH: 7.35 to 7.45 o Separated and nonhemolyzed sample are stable for 8 hours at 25º
- Specific gravity: 1.048 – 1.066 at 2 to 8º C. variable stability is observed in longer storage perio
o Serum: 1.026 – 1.031 o Glycolysis decreases serum glucose by approximately 5 to 10 mg/
o RBC: 1.092 – 1.095 normal uncentrifuged coagulated blood at room temperature. T
- Volume: 5 – 6 Liters (7 to 8% of total body weight) vitro glycolysis is higher in the presence of leukocytosis
o Males: 76 mL/kg body weight contamination.
o Females: 68 mL/kg body weight o Plasma, removed from the cells after moderate centrifugat
________________________________________________________________ leukocytes that also metabolize glucose, though cell-free sterile
glycolytic activity.
GLUCOSE: o Glycolysis can be inhibited and glucose stabilized for as long as 3
• Intended Use: temperature by adding sodium iodoacetate or sodium fluoride
o Reagents for quantitative in vitro determination specimen. Although iodide maintains long term blood glucose stab
• Summary of Test of decline in the first hour after sample collection is altered.
o Glucose is the primary energy source for the human body. It is derived from o CSF may be contaminated with bacteria or other cells and shou
the breakdown of carbohydrates in the diet and in the body stores, as well as for glucose immediately. If a delay in measurement is unavoidab
endogenous synthesis from protein or the glycerol moiety of triglycerides. should be centrifuged and stored at 4º C 0r-20ºC.
• Principle of the Method o Fresh collections of urine, glucose may be preserved by adding
o The enzyme glucose oxidase catalyzes the oxidation of glucose to gluconic acid acetic acid to the container before adding the collection. The fi
and H2O2. The H2O2 reacts with phenol and 4-aminoantipyrine in the presence urine is usually between 4 and 5, which inhibits bacterial activi
of peroxidase to form a quinoneimine dye. The intensity of the color formed is lose as much as 40% of their glucose at room temperature.
proportional to the glucose concentration and can be measured photometrically • Test procedure
between 480 to 520 nm.
• Kit Components Wavelength 510nm (allowed 480 – 520 nm
o For IN VITRO diagnostic use only. Light path 1 cm
o The components for the kit are stable until expiration date on the label. Temperature 37ºC
o Keep away from direct light sources. Dispense Blank Standard Sample
o GLU R1 F400: 4 x 100 mL (Liquid) blue cap Reagent 1 ml 1 ml 1 ml
100F 4 x 250 mL (Liquid) blue cap Water 10 µl - -
o Composition: Phosphate buffer pH 6.50 220 Mm, GOD > 15000U/l, POD > Standard - 10 µl -
5OOU/l, 4-AAP Mm, Phenol 10Mm, Surfactant. Sample - - 10 µl
o Standard: Glucose solution 100mg/dL – 5 mL Mix, Incubate at 37º C for 5 minutes.
o Store all components at 2 to 8ºC Read absorbance of Standard (As) and Samples (Ax) against reagent bla
• Liquid portion of an unclotted blood • Liquid portion of clotted blood o Current laboratory instrumentation. Spectrophotometer U
• Contains the fibrinogen group of • Does not contain the fibrinogen group thermostatic cuvette holder. Automatic micropipettes. Glass o
coagulation factors of coagulation factors polystyrene cuvettes. Saline solution.
• Lighter yellow and a little turbid • Darker yellow and clearer • Reagent preparation
• Chemically, does not contain much of • Contains higher levels of platelet o Use reagent ready to use
platelet derivatives. derivatives o Stability: Up to expiration date on labels at 2 to 8º C
o Stability since first opening of vials: Preferably 60 days at 2 to 8
Characteristics of Blood: • Specimen
• Physical o Serum, Plasma, Urine, CSF
- pH: 7.35 to 7.45 o Separated and nonhemolyzed sample are stable for 8 hours at 25º
- Specific gravity: 1.048 – 1.066 at 2 to 8º C. variable stability is observed in longer storage perio
o Serum: 1.026 – 1.031 o Glycolysis decreases serum glucose by approximately 5 to 10 mg/
o RBC: 1.092 – 1.095 normal uncentrifuged coagulated blood at room temperature. T
- Volume: 5 – 6 Liters (7 to 8% of total body weight) vitro glycolysis is higher in the presence of leukocytosis
o Males: 76 mL/kg body weight contamination.
o Females: 68 mL/kg body weight o Plasma, removed from the cells after moderate centrifugat
________________________________________________________________ leukocytes that also metabolize glucose, though cell-free sterile
glycolytic activity.
GLUCOSE: o Glycolysis can be inhibited and glucose stabilized for as long as 3
• Intended Use: temperature by adding sodium iodoacetate or sodium fluoride
o Reagents for quantitative in vitro determination specimen. Although iodide maintains long term blood glucose stab
• Summary of Test of decline in the first hour after sample collection is altered.
o Glucose is the primary energy source for the human body. It is derived from o CSF may be contaminated with bacteria or other cells and shou
the breakdown of carbohydrates in the diet and in the body stores, as well as for glucose immediately. If a delay in measurement is unavoidab
endogenous synthesis from protein or the glycerol moiety of triglycerides. should be centrifuged and stored at 4º C 0r-20ºC.
• Principle of the Method o Fresh collections of urine, glucose may be preserved by adding
o The enzyme glucose oxidase catalyzes the oxidation of glucose to gluconic acid acetic acid to the container before adding the collection. The fi
and H2O2. The H2O2 reacts with phenol and 4-aminoantipyrine in the presence urine is usually between 4 and 5, which inhibits bacterial activi
of peroxidase to form a quinoneimine dye. The intensity of the color formed is lose as much as 40% of their glucose at room temperature.
proportional to the glucose concentration and can be measured photometrically • Test procedure
between 480 to 520 nm.
• Kit Components Wavelength 510nm (allowed 480 – 520 nm
o For IN VITRO diagnostic use only. Light path 1 cm
o The components for the kit are stable until expiration date on the label. Temperature 37ºC
o Keep away from direct light sources. Dispense Blank Standard Sample
o GLU R1 F400: 4 x 100 mL (Liquid) blue cap Reagent 1 ml 1 ml 1 ml
100F 4 x 250 mL (Liquid) blue cap Water 10 µl - -
o Composition: Phosphate buffer pH 6.50 220 Mm, GOD > 15000U/l, POD > Standard - 10 µl -
5OOU/l, 4-AAP Mm, Phenol 10Mm, Surfactant. Sample - - 10 µl
o Standard: Glucose solution 100mg/dL – 5 mL Mix, Incubate at 37º C for 5 minutes.
o Store all components at 2 to 8ºC Read absorbance of Standard (As) and Samples (Ax) against reagent bla
