MICROBIOLOGY
AN EVOLVING
SCIENCE, 4TH
EDITION, JOHN W.
FOSTER, JOAN L.
SLONCZEWSKI
,CHAPTER 2: Observing the Microbial Cell
MULTIPLE CHOICE
1. Chlamydia trachomatis transmits infection to a new cell via
a. a membrane vesicle. d. host membranes.
b. elementary bodies. e. the human cell.
c. amoeba.
ANS: B DIF: Easy REF: 2.Intro
OBJ: 2.1d Identify the type(s) of microscopy needed to view a particular specimen
MSC: Remembering
2. The part of the human eye that is MOST involved in resolving an image is the
a. iris. d. retina.
b. lens. e. cornea.
c. optic nerve.
ANS: D DIF: Easy REF: 2.1
OBJ: 2.1a Explain how the structure of the human eye dictates the resolution of objects
MSC: Remembering
3. A ball-shaped microbe is referred to as a
a. bacillus. d. strepto.
b. coccus. e. spirochete.
c. vibrio.
ANS: B DIF: Easy REF: 2.1
OBJ: 2.1c Describe and identify bacterial morphologies (e.g., bacilli, cocci, and spirochetes)
MSC: Remembering
4. Resolution is the smallest distance by which two objects can be ________ and still be ________.
a. magnified; seen d. distinguished; separated
b. separated; distinguished e. magnified; distinguished
c. magnified; separated
ANS: B DIF: Medium REF: 2.1
OBJ: 2.1b Differentiate between resolution and detection MSC: Remembering
5. 400 nm is equivalent to
a. 4.0 105 m. d. 4.0 108 m.
b. 4.0 106 m. e. 4.0 109 m.
c. 4.0 107 m.
ANS: C DIF: Medium REF: 2.1
OBJ: 2.1a Explain how the structure of the human eye dictates the resolution of objects
MSC: Applying
6. Which of these series arranges microbes from smallest to largest?
a. virus bacterium red blood cell paramecium
b. virus red blood cell bacterium paramecium
c. bacterium virus paramecium red blood cell
d. bacterium virus red blood cell paramecium
e. paramecium red blood cell bacterium virus
, ANS: A DIF: Difficult REF: 2.1
OBJ: 2.1c Describe and identify bacterial morphologies (e.g., bacilli, cocci, and spirochetes)
MSC: Applying
7. What happens if the wavelength of radiation is larger than the object being viewed?
a. absorption d. refraction
b. scattering e. resolution
c. no resolution
ANS: C DIF: Easy REF: 2.2
OBJ: 2.2a Identify what conditions must exist for electromagnetic radiation to resolve an object
from neighboring objects or the surrounding medium MSC: Remembering
8. Wavelength interference results in small observed objects (like bacteria) being surrounded by
a. a capsule. d. a dark field.
b. a membrane. e. a cell wall.
c. concentric rings.
ANS: C DIF: Difficult REF: 2.2
OBJ: 2.2b Explain the properties of light MSC: Applying
9. What is the MOST important property that enables a lens to magnify an image?
a. absorption d. refraction
b. fluorescence e. scattering
c. reflection
ANS: D DIF: Easy REF: 2.2
OBJ: 2.2c Differentiate among absorption, reflection, refraction, and scattering
MSC: Remembering
10. When two waves are out of phase by ________ wavelength, they produce destructive interference,
canceling each other’s amplitude and resulting in contrast in the image.
a. one-tenth of a d. one-half of a
b. one-eighth of a e. one
c. one-quarter of a
ANS: D DIF: Difficult REF: 2.2
OBJ: 2.2d Explain how lenses magnify images MSC: Understanding
11. Increasing the refractive index of the medium between the object and the objective lens increases
a. refraction. d. resolution.
b. reflection. e. wavelength.
c. magnification.
ANS: D DIF: Difficult REF: 2.2
OBJ: 2.2d Explain how lenses magnify images MSC: Understanding
12. If aqueous cytoplasm was submerged in a beaker of immersion oil, the slide would be
a. undetectable. d. fluorescent.
b. brighter than its surroundings. e. stained.
c. darker than its surroundings.
ANS: A DIF: Medium REF: 2.2
OBJ: 2.2b Explain the properties of light MSC: Applying