EVERYTHING YOU RESEARCH NEEDS TO BE REFERENCED.
● An introduction to the assignment, what you will be looking at in your report
(use assignment brief context).
● An introduction into how chromatography works, what it is used for, what
factors affect separation, the difference between paper and TLC (differences,
when would they be used and why), different ways to show stains on
chromatograms (UV light, ninhydrin etc.)
● Risk assessment for chromatography
● What steps in your method and preparation were done to get the most
accurate results, how would you stop streaking and slanting?
● Method used for paper chromatography of plant pigments
● Results including pictures showing CLEAR SEPARATION (repeat until you have
them)(measurements of solvent fronts and sample fronts)
● Calculation of Rf values
● Determine which pigments are what in the leaf pigments based on literature
Rf values and colours
● Method used for TLC of plant pigments
● Results including pictures showing CLEAR SEPARATION(repeat until you have
them) (measurements of solvent fronts and sample fronts)
● Calculation of Rf values
● Determine which pigments are what in the leaf pigments based on literature
Rf values and colours
● Compare the two chromatographs for leaf pigments, which was more accurate
to literature values, why? Which showed clearer separation? Did you find any
differences? What errors could have caused this?
● Compare your results with others in the class (use numbers of Rf values), talk
about accuracy of results
● Which substances in your mixture travelled the furthest and the least? Why is
this? What factors caused this? (polarity, size, shape, etc.)
● If you were to redo the practical how would you change the METHOD to make
it more accurate, why would they increase the
accuracy/reliability/repeatability/precision? Justify the method steps you did to
make your practical accurate
● What would each improvement do to your results? How would it change them?
● If you were to redo the practical how would you change the EQUIPMENT to
make it more accurate, why would they increase the
accuracy/reliability/repeatability/precision? Justify the equipment you used to
make your practical accurate
● What would each improvement do to your results? How would it change them?
● Method used for paper chromatography of amino acids and an unknown
mixture
● Results including pictures showing CLEAR SEPARATION (repeat until you have
them) (measurements of solvent fronts and sample fronts)
● Calculation of Rf values
● Determine how accurate your controls Rf values by comparing them to
literature values and colours
● Determine what the unknown amino acids in the mixture were
● An introduction to the assignment, what you will be looking at in your report
(use assignment brief context).
● An introduction into how chromatography works, what it is used for, what
factors affect separation, the difference between paper and TLC (differences,
when would they be used and why), different ways to show stains on
chromatograms (UV light, ninhydrin etc.)
● Risk assessment for chromatography
● What steps in your method and preparation were done to get the most
accurate results, how would you stop streaking and slanting?
● Method used for paper chromatography of plant pigments
● Results including pictures showing CLEAR SEPARATION (repeat until you have
them)(measurements of solvent fronts and sample fronts)
● Calculation of Rf values
● Determine which pigments are what in the leaf pigments based on literature
Rf values and colours
● Method used for TLC of plant pigments
● Results including pictures showing CLEAR SEPARATION(repeat until you have
them) (measurements of solvent fronts and sample fronts)
● Calculation of Rf values
● Determine which pigments are what in the leaf pigments based on literature
Rf values and colours
● Compare the two chromatographs for leaf pigments, which was more accurate
to literature values, why? Which showed clearer separation? Did you find any
differences? What errors could have caused this?
● Compare your results with others in the class (use numbers of Rf values), talk
about accuracy of results
● Which substances in your mixture travelled the furthest and the least? Why is
this? What factors caused this? (polarity, size, shape, etc.)
● If you were to redo the practical how would you change the METHOD to make
it more accurate, why would they increase the
accuracy/reliability/repeatability/precision? Justify the method steps you did to
make your practical accurate
● What would each improvement do to your results? How would it change them?
● If you were to redo the practical how would you change the EQUIPMENT to
make it more accurate, why would they increase the
accuracy/reliability/repeatability/precision? Justify the equipment you used to
make your practical accurate
● What would each improvement do to your results? How would it change them?
● Method used for paper chromatography of amino acids and an unknown
mixture
● Results including pictures showing CLEAR SEPARATION (repeat until you have
them) (measurements of solvent fronts and sample fronts)
● Calculation of Rf values
● Determine how accurate your controls Rf values by comparing them to
literature values and colours
● Determine what the unknown amino acids in the mixture were
