BIOS 242 Week 2 iLab: Aseptic Technique (Findings and Explanations)

BIOS 242 Week 2 iLab: Aseptic Technique (Findings and Explanations) The purpose of this lab is to culture cells successfully without contamination using aseptic technique. If contamination occurs, the lab experiment will be inaccurate. Biological contamination can happen via dirty supplies, airborne toxins, and unkempt workstations. This aseptic technique lab is designed to guide you through a successful (uncontaminated) lab experiment. With these aseptic techniques it will help to yield a level of protection for the cell culture. Aseptic techniques require sterile media, sterile agents, proper hygiene, and a clean workspace. The case study involved a person named Manuel. Manuel surveyed soil microbes in soil. Findings: Mannitol Fermentation test 100% positive. Discussion Observations and Results Part I: Contamination Part II: Aseptic Transfer Tube-to-Tube Test Procedure Conclusion BACTERIAL ANATOMY Introduction The purpose of the bacterial anatomy experiment is to understand the anatomy of bacterial cells. This experiment is designed to find different structures that are linked to bacteria. Case study involves: A gal named Denice. Denice does an experiment centered around how the skin microflora changes in immunocompromised hamsters. She used both compromised and normal hamsters as her test subjects in the same environment. After two weeks both hamsters had the same organism found on their skin. Procedure Observations and Results Experiment 1. Experiment 2. Discussion Conclusion