BIO99 1/3 Questions With Complete Solutions
after helicase unwinds, there are 2 DNA strands, both unstable
due to no complementary H bonding, have supercoiling, and are
succeptible to nuclease breakdown. what enzyme solves this
problem? Correct Answers SSB. DNA wraps around SSB,
getting rid of supercoiling, and providing a stable protein/DNA
complex that cannot be cleaved by nuclease
are nucleotide bases hydrophobic or hydrophilic? where do they
face? Correct Answers hydrophobic, so they face inside.
are the backbones of DNA and RNA hydrophobic or
hydrophilic? what is their relationship with water? Correct
Answers the backbones of DNA and RNA are hydrophilic and
so H-bond with water.
can chromosomes be made of RNA? Correct Answers yes.
viruses have an RNA genome.
can we have secondary structures during replication? Correct
Answers no. secondary structures such as supercoiling cannot
be present while replicating DNA.
conservative model: semi: dispersive: Correct Answers
photocopy, normal, random as hell.
difference between RNA and DNA nucleotides? Correct
Answers RNA nucleotides have a 3' AND 2' OH group,
whereas DNA nucleotides only have a 3' OH.
, differences between PCR and in vivo replication? Correct
Answers DNA vs RNA primers. heat vs helicase, Taq pol vs pol
III. no need ligase, no okazaki frags.
DNA in a cell is found as: Correct Answers DNA in a cell is
found as chromatin, a DNA protein complex, not naked DNA.
do all genes code for proteins? Correct Answers no, so 30k
genes do not code for 30k proteins.
do we have similar basic genes as bacteria? Correct Answers
yes
does more genes/larger genome equate to more complex
organisms? Correct Answers no.
does raising pH weaken or strengthen the H--bonds between
nucleotide bases? Correct Answers raising pH weakens the H-
bonds between bases because making the pH basic means
reducing the number of hydrogens, thereby reducing the # of H-
bonds between the base pairs. This obviously ill weaken the
DNA structure, decreasing Tm
evidence of DNA packaging: Correct Answers chromatin and
small amounts of nuclease are mixed.the results are base pairs of
fragment length in multiples of 200, meaning a histone has
200bp fragments wrapped around it.
exceptions from central dogma: Correct Answers reverse
transcription, telomerase
after helicase unwinds, there are 2 DNA strands, both unstable
due to no complementary H bonding, have supercoiling, and are
succeptible to nuclease breakdown. what enzyme solves this
problem? Correct Answers SSB. DNA wraps around SSB,
getting rid of supercoiling, and providing a stable protein/DNA
complex that cannot be cleaved by nuclease
are nucleotide bases hydrophobic or hydrophilic? where do they
face? Correct Answers hydrophobic, so they face inside.
are the backbones of DNA and RNA hydrophobic or
hydrophilic? what is their relationship with water? Correct
Answers the backbones of DNA and RNA are hydrophilic and
so H-bond with water.
can chromosomes be made of RNA? Correct Answers yes.
viruses have an RNA genome.
can we have secondary structures during replication? Correct
Answers no. secondary structures such as supercoiling cannot
be present while replicating DNA.
conservative model: semi: dispersive: Correct Answers
photocopy, normal, random as hell.
difference between RNA and DNA nucleotides? Correct
Answers RNA nucleotides have a 3' AND 2' OH group,
whereas DNA nucleotides only have a 3' OH.
, differences between PCR and in vivo replication? Correct
Answers DNA vs RNA primers. heat vs helicase, Taq pol vs pol
III. no need ligase, no okazaki frags.
DNA in a cell is found as: Correct Answers DNA in a cell is
found as chromatin, a DNA protein complex, not naked DNA.
do all genes code for proteins? Correct Answers no, so 30k
genes do not code for 30k proteins.
do we have similar basic genes as bacteria? Correct Answers
yes
does more genes/larger genome equate to more complex
organisms? Correct Answers no.
does raising pH weaken or strengthen the H--bonds between
nucleotide bases? Correct Answers raising pH weakens the H-
bonds between bases because making the pH basic means
reducing the number of hydrogens, thereby reducing the # of H-
bonds between the base pairs. This obviously ill weaken the
DNA structure, decreasing Tm
evidence of DNA packaging: Correct Answers chromatin and
small amounts of nuclease are mixed.the results are base pairs of
fragment length in multiples of 200, meaning a histone has
200bp fragments wrapped around it.
exceptions from central dogma: Correct Answers reverse
transcription, telomerase
