BIOD 171 Lab Exam with solutions- notebook
Unknown pathogen analysis Latest
What three elements are used in an autoclave to sterilize equipment -
heat, pressure, and steam
what is the minimum temperature an autoclave must be set to achieve sterile
condition - 125 degrees Celsius
if you are working in a lab in which an autoclave is not available and you are
pressured for time what would you choose the best sterilizer equipment hot
steam or hot air explain why - hot steam is the best choice as you can
achieve a sterile environment in a matter of minutes whereas hot air will take
several hours to achieve the same goal
Study incubator tops pictures -
What are four types of gloves presented in the lab video - nitrile latex
thermal cold thermal heat
at what temperature here's the fix incubator set to as presented in the lab video -
37 degrees Celsius
What three roles were discussed in regards to lab safety that would protect you
and others from contamination - never eat or drink in the lab, always wear
appropriate PPE, never leave the lab wearing PPE
,BIOD 171 Lab Exam with solutions- notebook
Unknown pathogen analysis Latest
what are the main sections that should be found in a lab notebook? Name at least
4- objective, procedure, notes, results and deviations
at what temperature should you refrigerate bacterial samples explain why this is
beneficial - 4 degrees Celsius this is beneficial to slow bacterial growth and
prolongs the life of the sample
You are a lab instructor and Sally Miller has turned in her lab safety notebook for
you to grade the experiment number one online safety based upon what is
covered in the lab video how should Sally title her experiment - SM01 lab
safety
You arrived to your first day of work at a new lab you are taking over for someone
who took a new job at another lab your boss informs you that because of time
restraints this person did not exactly follow experimental protocol in order to
proceed you must know what he did differently one according to the lab lecture
under what section of his notebook would you look to find the experimental steps
two as changes in the experimental steps are made what are these differences
called and how should it appear in your lab notebook - number one
procedure this is where the steps for the experimental protocol are recorded, 2
deviations all deviations should be written in red to immediately bring attention
to changes in their protocol
Study parts of the microscope -
How should you hold the microscope when you move it or carry it - you
should hold it with one hand on the neck and one hand under the base
,BIOD 171 Lab Exam with solutions- notebook
Unknown pathogen analysis Latest
what two parts of the microscope contribute to the total magnification to your
sample - objective and oculars eye piece
As you're looking through the microscope you wish to dim or limit the amount
light entering the eye what component of the microscope other than the lot
source itself can be adjusted to make these modifications - iris diaphragm
what objective power is best used if you are uncertain what the sample is and
where to begin - 4x (or lowest power objective)
You are viewing a bacterial sample that is 7 micrometers in diameter through
your oil objective lens which has a magnification of 100 X the eyepiece has a
magnification power of 5X what size would the sample appear through the
eyepiece - 3500 micrometers or 3.5 millimeters in diameter or 500x larger)
based on the microscope shown in the lab lecture what objectives would require
replacing oil on this lod in order to clearly visualize the sample - 100 X
List the four main steps used to repair a wet mount and indicate which if any step
is optional - clean the slide
circle area on slide with wax or hydrophobic pen
apply Organism to the slide
view under microscope (note there is no optional steps in the wet mount)
, BIOD 171 Lab Exam with solutions- notebook
Unknown pathogen analysis Latest
when preparing a glass slide for a dry mouth what cleaning solution is used -
70% ethanol
when performing a wet mount technique what is the advantage of using a wax or
hydrophobic pen - creating a hydrophobic barrier helps keep the water
within the circle so it does not spill off the slide
What dye did gram negative bacteria primarily retain - safranin pink in
color
why are gram positive bacteria able to retain the crystal Violet dye - they
contain a thick peptidoglycan layer in their cell wall that readily retains the dye
Study gram status a bacterial pictures -
An acid fast stain is most commonly used to identify what type of bacterium what
is the name of the primary dye used in this technique - mycobacterium,
carbolfushin dye (red)
Why do bacteria repel the dye nigrosine - nigrosin is a negatively charged I
the membranes of most cells are also negatively charged the membrane will repel
the die and not allow it to be absorbed
Unknown pathogen analysis Latest
What three elements are used in an autoclave to sterilize equipment -
heat, pressure, and steam
what is the minimum temperature an autoclave must be set to achieve sterile
condition - 125 degrees Celsius
if you are working in a lab in which an autoclave is not available and you are
pressured for time what would you choose the best sterilizer equipment hot
steam or hot air explain why - hot steam is the best choice as you can
achieve a sterile environment in a matter of minutes whereas hot air will take
several hours to achieve the same goal
Study incubator tops pictures -
What are four types of gloves presented in the lab video - nitrile latex
thermal cold thermal heat
at what temperature here's the fix incubator set to as presented in the lab video -
37 degrees Celsius
What three roles were discussed in regards to lab safety that would protect you
and others from contamination - never eat or drink in the lab, always wear
appropriate PPE, never leave the lab wearing PPE
,BIOD 171 Lab Exam with solutions- notebook
Unknown pathogen analysis Latest
what are the main sections that should be found in a lab notebook? Name at least
4- objective, procedure, notes, results and deviations
at what temperature should you refrigerate bacterial samples explain why this is
beneficial - 4 degrees Celsius this is beneficial to slow bacterial growth and
prolongs the life of the sample
You are a lab instructor and Sally Miller has turned in her lab safety notebook for
you to grade the experiment number one online safety based upon what is
covered in the lab video how should Sally title her experiment - SM01 lab
safety
You arrived to your first day of work at a new lab you are taking over for someone
who took a new job at another lab your boss informs you that because of time
restraints this person did not exactly follow experimental protocol in order to
proceed you must know what he did differently one according to the lab lecture
under what section of his notebook would you look to find the experimental steps
two as changes in the experimental steps are made what are these differences
called and how should it appear in your lab notebook - number one
procedure this is where the steps for the experimental protocol are recorded, 2
deviations all deviations should be written in red to immediately bring attention
to changes in their protocol
Study parts of the microscope -
How should you hold the microscope when you move it or carry it - you
should hold it with one hand on the neck and one hand under the base
,BIOD 171 Lab Exam with solutions- notebook
Unknown pathogen analysis Latest
what two parts of the microscope contribute to the total magnification to your
sample - objective and oculars eye piece
As you're looking through the microscope you wish to dim or limit the amount
light entering the eye what component of the microscope other than the lot
source itself can be adjusted to make these modifications - iris diaphragm
what objective power is best used if you are uncertain what the sample is and
where to begin - 4x (or lowest power objective)
You are viewing a bacterial sample that is 7 micrometers in diameter through
your oil objective lens which has a magnification of 100 X the eyepiece has a
magnification power of 5X what size would the sample appear through the
eyepiece - 3500 micrometers or 3.5 millimeters in diameter or 500x larger)
based on the microscope shown in the lab lecture what objectives would require
replacing oil on this lod in order to clearly visualize the sample - 100 X
List the four main steps used to repair a wet mount and indicate which if any step
is optional - clean the slide
circle area on slide with wax or hydrophobic pen
apply Organism to the slide
view under microscope (note there is no optional steps in the wet mount)
, BIOD 171 Lab Exam with solutions- notebook
Unknown pathogen analysis Latest
when preparing a glass slide for a dry mouth what cleaning solution is used -
70% ethanol
when performing a wet mount technique what is the advantage of using a wax or
hydrophobic pen - creating a hydrophobic barrier helps keep the water
within the circle so it does not spill off the slide
What dye did gram negative bacteria primarily retain - safranin pink in
color
why are gram positive bacteria able to retain the crystal Violet dye - they
contain a thick peptidoglycan layer in their cell wall that readily retains the dye
Study gram status a bacterial pictures -
An acid fast stain is most commonly used to identify what type of bacterium what
is the name of the primary dye used in this technique - mycobacterium,
carbolfushin dye (red)
Why do bacteria repel the dye nigrosine - nigrosin is a negatively charged I
the membranes of most cells are also negatively charged the membrane will repel
the die and not allow it to be absorbed
