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MLT ASCP Practice Exam Questions with Verified Answers – Complete Medical Laboratory Technician Review Guide

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Prepare effectively for your Medical Laboratory Technician (MLT) ASCP exams with this comprehensive study guide. This PDF contains: Practice questions with verified answers for each major MLT topic Detailed rationales to help you understand the reasoning behind each answer Coverage of essential areas including hematology, clinical chemistry, microbiology, immunology, molecular diagnostics, and lab safety Designed for self-study, exam prep, and board certification review Helps reinforce knowledge, improve critical thinking, and boost exam confidence Whether you are a student preparing for the ASCP board exam, a recent graduate, or a working laboratory professional, this guide will help you master key concepts, understand testing principles, and excel in both theoretical and practical components of your MLT certification. Benefits of this guide: Step-by-step explanations for every question Perfect for review before exams Covers frequently tested topics and challenging concepts Ideal for US and international MLT exam preparation

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Uploaded on
February 2, 2026
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2025/2026
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MLT ASCP Practice Exam Questions with Verified
Answers | Full Medical Laboratory Technician Review


LAB SAFETY, HEMATOLOGY & IMMUNOLOGY

Questions with Correct Answers and rationales



1. Standard precautions apply to all of the following except:

a. Blood
b. Cerebrospinal fluid
c. Semen
d. Concentrated acids ✅

Rationale:
Standard precautions apply to blood and body fluids that may contain infectious
agents. Concentrated acids are chemical hazards, not biological, and therefore are
excluded.



2. The most important practice in preventing the spread of disease is:

a. Wearing masks during patient contact
b. Proper hand washing ✅
c. Wearing disposable laboratory coats
d. Identifying specimens from known or suspected HIV- and HBV-infected
patients with a red label

Rationale:
Hand washing is the most effective way to prevent transmission of
microorganisms and interrupt the chain of infection.

,3. The appropriate dilution of bleach to use in laboratory disinfection is:

a. 1:2
b. 1:5
c. 1:10 ✅
d. 1:100

Rationale:
A 1:10 bleach dilution provides sufficient chlorine concentration for effective
laboratory disinfection.



4. How frequently should fire alarms and sprinkler systems be tested?

a. Weekly
b. Monthly
c. Quarterly ✅
d. Annually

Rationale:
Fire safety systems are routinely tested quarterly to ensure reliability and
compliance with safety regulations.



5. Where should alcohol and other flammable chemicals be stored?

a. In an approved safety can or storage cabinet away from heat sources ✅
b. Under a hood and arranged alphabetically
c. In a refrigerator at 2°C to 8°C
d. On a low shelf protected from light

Rationale:
Flammable chemicals must be stored in approved safety cabinets to reduce fire
risk.



6. The most frequent cause of needle punctures is:

, a. Patient movement during venipuncture
b. Improper disposal of phlebotomy equipment ✅
c. Inattention during removal of needle
d. Failure to attach needle firmly

Rationale:
Most needlestick injuries occur after use, particularly during disposal of sharps.



7. What class fire extinguisher would be appropriate to use on a fire in a
chemical cabinet?

a. Class A
b. Class B ✅
c. Class C
d. Class D

Rationale:
Class B extinguishers are used for flammable liquids and chemical fires.



8. Select the needle most commonly used in standard venipuncture in an
adult:

a. One inch, 18 gauge
b. One inch, 21 gauge ✅
c. One-half inch, 23 gauge
d. One-half inch, 25 gauge

Rationale:
A 21-gauge needle provides optimal blood flow with minimal vein trauma.



9. The process of formation and development of blood cells is termed:

a. Hematopoiesis ✅
b. Hematemesis
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