Portage Microbiology BIOD171 Exam 5 Portage Microbiology BIOD171 Exam 5.pdf
Portage Microbiology BIOD171 Exam 5
Universal precautions means any and all samples, whether known or
unknown, are to be treated as potentially hazardous (or pathogenic)
materials.
Define the concept of universal precautions.
A lab researcher would be certain to note:
Size and shape
Any observable motility
Gram status (positive or negative)
The presence of any chemical reactions
Changes in color localized to the organism or the surrounding media
Capture (or draw) images of any of the characteristics described above
List at least 3 observations a researcher would be sure to note while
assessing an unknown microbial sample.
You must determine motility before determining the Gram status. Motility
requires a wet mount, while Gram staining requires heat fixing the sample.
If one were to begin with the Gram stain the heat fixation process would kill
the organism, making any observations regarding motility impossible. The
correct approach would be to place the liquid culture on a glass slide and
Portage Microbiology BIOD171 Exam 5.pdf Portage Microbiology BIOD171 Exam
Portage
5 Microbiology BIOD171 Exam 5.pdf/ 2026 with Solution
, Portage Microbiology BIOD171 Exam 5.pdf/ 2026 with Solution
Portage Microbiology BIOD171 Exam 5 Portage Microbiology BIOD171 Exam 5.pdf
determine its motility status. Next, the same liquid culture can be heat fixed
and Gram stained.
While observing an unknown sample of limited amounts, a researcher must
determine the following observations: (1) the presence of any motility and
(2) its Gram status using the same sample—the liquid sample cannot be
divided. Which would you determine first and why?
A facultative anaerobe is capable of growth under aerobic (with oxygen)
and anaerobic (without oxygen) conditions.
A facultative anaerobe is a microorganism capable of growth under what
conditions?
Streptococcus is catalse negative which means it cannot break down
peroxides and if any peroxides are present it would die.
As Streptococcus is catalase negative would it thrive or die in the presence
of peroxides? Why?
Blood agar
Strep is often cultured on Blood agar plates to determine its hemolytic
properties, which aids in the classification (and differentiation) process.
Streptococcus is most often streaked onto:
Portage Microbiology BIOD171 Exam 5.pdf Portage Microbiology BIOD171 Exam
Portage
5 Microbiology BIOD171 Exam 5.pdf/ 2026 with Solution