TEST 2026 QUESTIONS WITH SOLUTIONS
GRADED A+
◉ BEA: Black
Salt: No Growth
Optochin: resistant. Answer: GDS
◉ BEA: Colorless
Salt: No Growth
Optochin: Susceptible. Answer: Streptococcus pneumoniae
◉ BEA: Coloreless
Salt: No Growth
Optochin: Resistant. Answer: Viridans Strep
◉ Hemolysis: Beta
Gram Stain: GP Coccobacilli
Catalase: +
BEA: +
CAMP/Hippurate: +
,Motility: + @ 25 C. Answer: Listeria
◉ What organisms are motile at 25 C?. Answer: Yersinia
entercolitica & Listeria
◉ What GPR organisms form spores?. Answer: Bacillus species
(aerobic)
Clostridium species (anaerobic)
◉ What temperature should CIN agar be incubated at?. Answer: RT
◉ Selective for Gram-Negative Bacilli. Answer: EMB & MAC
◉ Selective media for Gram-positive organisms. Answer: CNA & PEA
◉ Gram staining steps. Answer: 1. crystal violet
2. iodine
3. alcohol
4. safranin
◉ ID Of Staphylococci. Answer: -Slide coagulase
-Tube coagulase
, -MSA
-Latex agglutination
-Novobiocin
◉ Reagent: Bile Solubility. Answer: 10% deoxycholate (bile)
◉ Enhance growth of specific MOs, while inhibiting the growth of
others (GN & Selenite). Answer: Enrichment Broths
◉ No antimicrobial substances & allows growth of most organisms
(BAP, CHOC). Answer: Nonselective Media
◉ Contains antimicrobial substances, allows growth of specific
bacteria while inhibiting the growth of others (PEA, CNA). Answer:
Selective Media
◉ Basic growth media for bacteria (BAP/ CHOC). Answer: Nutrient
Media
◉ Metabolic differences and biochemical reactions in the media
distinguishes MO (ph Color indicator). Answer: Differential Media
◉ Reagent: Catalase. Answer: Hydrogen Peroxide