AAB MT Immunohematology EXAM STUDY GUIDE
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What is hemoglobin composed of? - (ANSWERS)4 heme, 4 iron, 4 globins
What disorder does a defect in heme synthesis lead to? - (ANSWERS)Porphyria
Porphyria - (ANSWERS)Deficiency in the enzymes of the porphyrin pathway leads
to insufficient production of heme. The principal problem in these deficiencies is the
accumulation of porphyrins, the heme precursors, which are toxic to tissue in high
concentrations.
What is methemoglobin? - (ANSWERS)Methemoglobin is a form of hemoglobin
that contains ferric [Fe3+] iron and has a decreased ability to bind oxygen. However,
the ferric iron has an increased affinity for bound oxygen. When methemoglobin
concentration is elevated in red blood cells, tissue hypoxia can occur.
What is the function of the enzyme methemoglobin-cytochrome C reductase in a red
blood cell? - (ANSWERS)To keep iron in the ferrous state
What product is formed when ferrous iron of hemoglobin is oxidized to the ferric
state? - (ANSWERS)Methemoglobin
What is carboxyhemoglobin? - (ANSWERS)A stable complex of carbon monoxide
and hemoglobin that forms in rbcs when carbon monoxide is inhaled or produced in
normal metabolism. Large quantities of it hinder delivery of O2 to the body.
Tobacco smoking (through carbon monoxide inhalation) raises the blood levels of
cohb by a factor of several times from its normal concentrations.
Which hemoglobin has a 200 times greater binding capacity for the hemoglobin
molecule than oxygen? - (ANSWERS)Carboxyhemoglobin
Which hemoglobin derivative causes irreversible changes in rbcs? -
, AAB MT Immunohematology EXAM STUDY GUIDE
2026/2027 ACCURATE QUESTIONS WITH CORRECT
DETAILED ANSWERS || 100% GUARANTEED PASS
<NEWEST VERSION>
(ANSWERS)Sulfhemoglobin -- makes blood incapable of carrying oxygen.
Which of the following hemoglobins is NOT normally present in a normal adult? -
(ANSWERS)Hb-S
Which embryonic hemoglobin is normally found in newborns? - (ANSWERS)Hb-F
What is the most abundant hemoglobin in a newborn? - (ANSWERS)Hb-F
What is the molecular structure of Hemoglobin S? - (ANSWERS)Valine replaces
glutamic acid in the 6th position of the beta chain
Which of the following hemoglobins is insoluble under lowered oxygen tension? -
(ANSWERS)Hb-S
What laboratory test is used to confirm the specific diagnosis of sickle cell anemia? -
(ANSWERS)Hemoglobin electrophoresis
In the solubility test for hemoglobin S, what is the reagent responsible for the
reduction of the hemoglobin molecule? - (ANSWERS)Sodium dithionite
In hemoglobin C, what replaces glutamic acid? - (ANSWERS)Lysine
Which red blood cell inclusion is characteristic of hemoglobin C disease? -
(ANSWERS)Rod-shaped crystals
What is hemoglobin F composed of? - (ANSWERS)2 alpha and 2 gamma chains
Which hemoglobin is resistant to alkali denaturation? - (ANSWERS)Hb-F
, AAB MT Immunohematology EXAM STUDY GUIDE
2026/2027 ACCURATE QUESTIONS WITH CORRECT
DETAILED ANSWERS || 100% GUARANTEED PASS
<NEWEST VERSION>
Which hemoglobins are differentiated by the Kleihauer-Betke technique? -
(ANSWERS)Hb-A1 from Hb-F
What is the order of migration of hemoglobins, from fastest to slowest, on cellulose
acetate at ph 8.4? - (ANSWERS)A1, F, S, C
Why is citrate agar used when abnormal hemoglobins are identified on cellulose
acetate at ph 8.6? - (ANSWERS)It separates hemoglobin S from hemoglobin D
The cyanmethemoglobin method measures all hemoglobin pigments EXCEPT
which one? - (ANSWERS)Sulfhemoglobin
What is the diluent used in the cyanmethemoglobin method for the determination of
hemoglobin? - (ANSWERS)Drabkin's solution
What is the normal plasma hemoglobin concentration? - (ANSWERS)2-3 mg/dl
Which plasma protein binds free hemoglobin? - (ANSWERS)Haptoglobin
Which one of the following statements concerning hemolytic anemia is false? -
(ANSWERS)The serum haptoglobin is increased
In this type of anemia rbcs are normocytic and normochromic. Rbcs, hemoglobin,
and hematocrit are decreased and the indirect bilirubin is increased. -
(ANSWERS)Hemolytic anemia
Myoglobin can be separated from hemoglobin by saturating the specimen with
which one of the following reagents? - (ANSWERS)Ammonium sulfite
The hematocrit is also known as which one of the following terms? -
2026/2027 ACCURATE QUESTIONS WITH CORRECT
DETAILED ANSWERS || 100% GUARANTEED PASS
<NEWEST VERSION>
What is hemoglobin composed of? - (ANSWERS)4 heme, 4 iron, 4 globins
What disorder does a defect in heme synthesis lead to? - (ANSWERS)Porphyria
Porphyria - (ANSWERS)Deficiency in the enzymes of the porphyrin pathway leads
to insufficient production of heme. The principal problem in these deficiencies is the
accumulation of porphyrins, the heme precursors, which are toxic to tissue in high
concentrations.
What is methemoglobin? - (ANSWERS)Methemoglobin is a form of hemoglobin
that contains ferric [Fe3+] iron and has a decreased ability to bind oxygen. However,
the ferric iron has an increased affinity for bound oxygen. When methemoglobin
concentration is elevated in red blood cells, tissue hypoxia can occur.
What is the function of the enzyme methemoglobin-cytochrome C reductase in a red
blood cell? - (ANSWERS)To keep iron in the ferrous state
What product is formed when ferrous iron of hemoglobin is oxidized to the ferric
state? - (ANSWERS)Methemoglobin
What is carboxyhemoglobin? - (ANSWERS)A stable complex of carbon monoxide
and hemoglobin that forms in rbcs when carbon monoxide is inhaled or produced in
normal metabolism. Large quantities of it hinder delivery of O2 to the body.
Tobacco smoking (through carbon monoxide inhalation) raises the blood levels of
cohb by a factor of several times from its normal concentrations.
Which hemoglobin has a 200 times greater binding capacity for the hemoglobin
molecule than oxygen? - (ANSWERS)Carboxyhemoglobin
Which hemoglobin derivative causes irreversible changes in rbcs? -
, AAB MT Immunohematology EXAM STUDY GUIDE
2026/2027 ACCURATE QUESTIONS WITH CORRECT
DETAILED ANSWERS || 100% GUARANTEED PASS
<NEWEST VERSION>
(ANSWERS)Sulfhemoglobin -- makes blood incapable of carrying oxygen.
Which of the following hemoglobins is NOT normally present in a normal adult? -
(ANSWERS)Hb-S
Which embryonic hemoglobin is normally found in newborns? - (ANSWERS)Hb-F
What is the most abundant hemoglobin in a newborn? - (ANSWERS)Hb-F
What is the molecular structure of Hemoglobin S? - (ANSWERS)Valine replaces
glutamic acid in the 6th position of the beta chain
Which of the following hemoglobins is insoluble under lowered oxygen tension? -
(ANSWERS)Hb-S
What laboratory test is used to confirm the specific diagnosis of sickle cell anemia? -
(ANSWERS)Hemoglobin electrophoresis
In the solubility test for hemoglobin S, what is the reagent responsible for the
reduction of the hemoglobin molecule? - (ANSWERS)Sodium dithionite
In hemoglobin C, what replaces glutamic acid? - (ANSWERS)Lysine
Which red blood cell inclusion is characteristic of hemoglobin C disease? -
(ANSWERS)Rod-shaped crystals
What is hemoglobin F composed of? - (ANSWERS)2 alpha and 2 gamma chains
Which hemoglobin is resistant to alkali denaturation? - (ANSWERS)Hb-F
, AAB MT Immunohematology EXAM STUDY GUIDE
2026/2027 ACCURATE QUESTIONS WITH CORRECT
DETAILED ANSWERS || 100% GUARANTEED PASS
<NEWEST VERSION>
Which hemoglobins are differentiated by the Kleihauer-Betke technique? -
(ANSWERS)Hb-A1 from Hb-F
What is the order of migration of hemoglobins, from fastest to slowest, on cellulose
acetate at ph 8.4? - (ANSWERS)A1, F, S, C
Why is citrate agar used when abnormal hemoglobins are identified on cellulose
acetate at ph 8.6? - (ANSWERS)It separates hemoglobin S from hemoglobin D
The cyanmethemoglobin method measures all hemoglobin pigments EXCEPT
which one? - (ANSWERS)Sulfhemoglobin
What is the diluent used in the cyanmethemoglobin method for the determination of
hemoglobin? - (ANSWERS)Drabkin's solution
What is the normal plasma hemoglobin concentration? - (ANSWERS)2-3 mg/dl
Which plasma protein binds free hemoglobin? - (ANSWERS)Haptoglobin
Which one of the following statements concerning hemolytic anemia is false? -
(ANSWERS)The serum haptoglobin is increased
In this type of anemia rbcs are normocytic and normochromic. Rbcs, hemoglobin,
and hematocrit are decreased and the indirect bilirubin is increased. -
(ANSWERS)Hemolytic anemia
Myoglobin can be separated from hemoglobin by saturating the specimen with
which one of the following reagents? - (ANSWERS)Ammonium sulfite
The hematocrit is also known as which one of the following terms? -
