Lab Practical 2 Questions with Correct
Answers
Independent variable in the Chlorophyll Extraction Lab? - ANSWERSWavelength, light
Dependent variable in the Chlorophyll Extraction Lab? - ANSWERSAbsorbency
What type of driven reaction is photosynthesis? - ANSWERSLight driven reaction
What is the relationship between energy and wavelength? - ANSWERSInverse. The
greater the energy then the higher the frequency and shorter the wavelength.
What light does chlorophyll absorb best? - ANSWERSBlue light
What light does chlorophyll A absorb best? - ANSWERSRed
400-450 wavelength color - ANSWERSBlue
500-550 wavelength color - ANSWERSGreen
700-750 wavelength color - ANSWERSRed
3 solvents for Chlorophyl Extraction Lab? - ANSWERSPetroleum ether, ethanol, &
methanol
Chlorophyll in petroleum ether? - ANSWERSChlorophyll A
Chlorophyll in ethanol? - ANSWERSCarotene
Chlorophyll in methanol? - ANSWERSChlorophyll B
What kind of density did petroleum ether have? - ANSWERSLow density
Was chlorophyll A stored in the top layer or bottom layer in the test tube? -
ANSWERSTop because it was in petroleum ether which had a low density.
What kind of density did methanol have? - ANSWERSHigh density
Was chlorophyll B stored in the top layer or bottom layer in the test tube? -
ANSWERSBottom because it was in methanol which had a high density.
, Independent variable of the Oxidation of Water Lab? - ANSWERSAmount of chloroplast
added
What does water split into? - ANSWERSO2 and H+
Dependent variable for the Oxidation of Water Lab? - ANSWERSRate at which water
was oxidized or the reduction of DPIP
Light hits which photosystem? - ANSWERSBoth
What is oxidation? - ANSWERSLoss of electrons
What is reduction? - ANSWERSGain of electrons
Is NADP+ reduced or oxidized? (Oxidation Lab) - ANSWERSOxidized
If DPIP is oxidized what happens? (Oxidation Lab) - ANSWERSIt is a blue color.
If DPIP is reduced what happens? (Oxidation Lab) - ANSWERSIt turns to clear.
What color wavelength does DPIP reflect? (Oxidation Lab) - ANSWERSBlue
wavelengths
mL of chloroplast (x) vs. rate of reaction (y) is what kind of relationship? (Oxidation Lab)
- ANSWERSDirect relationship, positive. More chloroplasts means a faster reaction.
Chloroplast has everything to make the reaction causing an increase in what?
(Oxidation Lab) - ANSWERSH2O oxidation
What two things play a factor in absorbance? (Oxidation Lab) - ANSWERSLight and
heat
Which factor (light or heat) played a larger role in driving the reaction? (Oxidation Lab) -
ANSWERSLight
What additive did the blank receive but the other solutions did not? (Oxidation Lab) -
ANSWERSBisulfide
What did bisulfide do in the blank? (Oxidation Lab) - ANSWERSIt changed the color to
clear
Independent variable in the Production of Oxygen Lab? - ANSWERSAmount of light
(PFR)
Dependent variable in the Production of Oxygen Lab? - ANSWERSRate of O2
production