MLS ASCP CERTIFICATION EXAM SCRIPT 2026
QUESTIONS WITH ANSWERS GRADED A+
◉ Patients with antibody to the following antigen are immune to
Hepatitis B: Answer: Surface antigen (HbSAg)
◉ Which two of the following tests are helpful for documenting
previous Streptococcal throat and skin infections: Answer: ASO titer and
Anti-DNase B
◉ In mass spectrometry, the "appearance potential" is defined as the:
Answer: Minimum energy required for the appearance of a particular
fragment ion in mass spectrum
◉ What is generally accepted as the lower threshold value for semen pH
from fertile males? Answer: 7.2
◉ Coulometry: Answer: An automated method for measuring chloride
which generates silver ions in the reaction
◉ Erythroleukemia: Answer: Also known as Acute Myelogenous
Leukemia type M6. This type is associated with either the presence of
both erythroid and myeloid precursors OR strictly erythroid precursors.
,◉ Myelomonocytic leukemia Answer: Is associated with an increase in
myeloid and monocytic cell precursors, but not red blood cell precursors
◉ Species that are Lysine (+), arginine (=) and ornithine (+): Answer:
Enterobacter aerogenes, Edwardsiella species and most Serratia species
◉ Lysine reaction for all Citrobacter: Answer: Negative
◉ Key identifying characteristic for Pantoa (Enterobacter) agglomerans:
Answer: negative reactions for the three decarboxylases commonly
tested ( Lysine, arginine and ornithine)
◉ Which organism is MOST likely responsible for septic reactions
associated with Red Blood Cell transfusions? Answer: Yersina
entercolitica is most likely responsible for septic reactions in
transfusions of Red Blood Cells. This organism is usually acquired by
ingestion of contaminated food and causes mild symptoms of abdominal
pain and diarrhea. Growth of Y. entercolitica is enhanced in iron-rich
environments such as red cell components.
◉ Gaucher's disease Answer: The most common lysosomal storage
disease caused by insufficient activity of the lysosomal enzyme
glucocerebrosidase that leads to the deposition of glucocerebroside in
cells of the macrophage-monocyte system.
, ◉ Diseases associated with dysfunctions of polymorphonuclear
neutrophils (PMNs) Answer: Chediak-Higashi syndrome (CH), Chronic
granulomatous disease (CGD), Myeloperoxidase deficiency (MPO)
◉ Nephrotic Syndrome: Answer: Caused by damage to the kidneys,
especially the basement membrane of the glomerulus; which causes
abnormal excretion of protein and red blood cells in the urine. Fats are
also present in the urine in most cases. A foamy appearance of the urine
is a key characteristic of this condition.
Clinical symptoms include:
Swelling
Weight gain from fluid retention
Poor appetite
High blood pressure
◉ Key Biochemical reactions for Enterobacteriaceae: Answer: The key
biochemical reactions by which the family Enterobacteriaceae can be
identified include fermentation of carbohydrates, reduction of nitrates to
nitrites and the absence of cytochrome oxidase activity.
◉ ANA Answer: See 2 quiz #27
QUESTIONS WITH ANSWERS GRADED A+
◉ Patients with antibody to the following antigen are immune to
Hepatitis B: Answer: Surface antigen (HbSAg)
◉ Which two of the following tests are helpful for documenting
previous Streptococcal throat and skin infections: Answer: ASO titer and
Anti-DNase B
◉ In mass spectrometry, the "appearance potential" is defined as the:
Answer: Minimum energy required for the appearance of a particular
fragment ion in mass spectrum
◉ What is generally accepted as the lower threshold value for semen pH
from fertile males? Answer: 7.2
◉ Coulometry: Answer: An automated method for measuring chloride
which generates silver ions in the reaction
◉ Erythroleukemia: Answer: Also known as Acute Myelogenous
Leukemia type M6. This type is associated with either the presence of
both erythroid and myeloid precursors OR strictly erythroid precursors.
,◉ Myelomonocytic leukemia Answer: Is associated with an increase in
myeloid and monocytic cell precursors, but not red blood cell precursors
◉ Species that are Lysine (+), arginine (=) and ornithine (+): Answer:
Enterobacter aerogenes, Edwardsiella species and most Serratia species
◉ Lysine reaction for all Citrobacter: Answer: Negative
◉ Key identifying characteristic for Pantoa (Enterobacter) agglomerans:
Answer: negative reactions for the three decarboxylases commonly
tested ( Lysine, arginine and ornithine)
◉ Which organism is MOST likely responsible for septic reactions
associated with Red Blood Cell transfusions? Answer: Yersina
entercolitica is most likely responsible for septic reactions in
transfusions of Red Blood Cells. This organism is usually acquired by
ingestion of contaminated food and causes mild symptoms of abdominal
pain and diarrhea. Growth of Y. entercolitica is enhanced in iron-rich
environments such as red cell components.
◉ Gaucher's disease Answer: The most common lysosomal storage
disease caused by insufficient activity of the lysosomal enzyme
glucocerebrosidase that leads to the deposition of glucocerebroside in
cells of the macrophage-monocyte system.
, ◉ Diseases associated with dysfunctions of polymorphonuclear
neutrophils (PMNs) Answer: Chediak-Higashi syndrome (CH), Chronic
granulomatous disease (CGD), Myeloperoxidase deficiency (MPO)
◉ Nephrotic Syndrome: Answer: Caused by damage to the kidneys,
especially the basement membrane of the glomerulus; which causes
abnormal excretion of protein and red blood cells in the urine. Fats are
also present in the urine in most cases. A foamy appearance of the urine
is a key characteristic of this condition.
Clinical symptoms include:
Swelling
Weight gain from fluid retention
Poor appetite
High blood pressure
◉ Key Biochemical reactions for Enterobacteriaceae: Answer: The key
biochemical reactions by which the family Enterobacteriaceae can be
identified include fermentation of carbohydrates, reduction of nitrates to
nitrites and the absence of cytochrome oxidase activity.
◉ ANA Answer: See 2 quiz #27
