Micro Final Exam Portage Learning | Questions and Correct Answers Verified 100% | Latest Update

Micro Final Exam Portage Learning | Questions and Correct Answers Verified 100% | Latest Update The process of photophosphorylation produces which of the following: select all that apply. NADPH H20 ATP CO2 Glyceraldehyde-3-phosphate - ANSWER -NADPH ATP In phosphorylation, the light reactions always occur where? - ANSWER Membrane True or False: The Calvin cycle must occur in the absence of light. - ANSWER False True or False: The distinguishing characteristic of Gram-Negative bacteria is the presence of LPS in the outer membrane. - ANSWER -True True or False: If you wish to study the motility of an organism you cannot heat fix, but you can chemically fix the specimen. - ANSWER -False both heat and chemical will kill the cell You want to observe the size and shape of a cell. What is the easiest staining technique that you could perform? Name at least one dye you would use during this process. - ANSWER -Simple stain and I would use crystal violet You suspect a patient may have TB. Once a sample has been obtained, it is sent off to the lab for an acid-fast stain. If the patient were infected with TB, describe what you would expect to see on the stained slide. - ANSWER -Red Cells on a blue background True or False: A Giemsa stain can be used to determine the presence of pathogenic bacteria. - ANSWER -True Identify what type of electron microscope was used to capture the following image and explain your choice (A). - ANSWER -Transmission Electron Microscope (TEM) This shows a 2D image of the internal structure, which is only shown by TEM. Identify what type of electron microscope was used to capture the following image and explain your choice (B). - ANSWER -Scanning electron microscope and we can tell based on the trademark shell that is shown in the 20 nm image. True or False. The purpose of a quadrant streak is to produce individual colonies of a bacterial population. - ANSWER -True A dilution gradient is formed when carrying out what generalize plating strategy? - ANSWER -Phase-Dilution streak In what phase of a dilution streak would you expect to find the highest concentration of bacteria, P2 or P4? - ANSWER -P2 True or False. When performing a dilution streak a new (or sterilized) loop is not required for each phase as long as the bacterial culture is pure. - ANSWER -False The number of phases (3 vs. 4) and/or the number of times a loop passes through a previous phase (once vs. multiple times) is acceptable provided what happens? - ANSWER -The resulting gradient has in it the growth of individual colonies. True or False. To restrict the growth of a pathogenic microbe a researcher might decrease an incubator from 37°C to 25°C. - ANSWER -True When given an unknown bacterial sample the first step is to expand the current bacterial population. Which form of media best suites this need? Why? - ANSWER -LB media because all of the other forms are selective media meaning they may potentially inhibit the growth of the unknown sample. True or False. Only directly ingesting the pre-formed clostridium toxin (neither the bacteria nor its spores) will cause disease in adults. - ANSWER -True True or False. There are currently no cures for tetanus. - ANSWER -True The alpha-toxin perfringolysin is associated with which medical condition caused by Gram-positive anaerobic bacteria? Gas gangrene Tetanus Leprosy Botulism - ANSWER -Gas gangrene True or False. Bubonic plague is often characterized by painfully swollen lymph nodes. - ANSWER -True List the following disease: - ANSWER -Anthrax True or False. Chlyamida is an obligate parasite and as such can be grown on agar plates alone. - ANSWER -False True or False. Similar to chickenpox, the blisters that appear with shingles can cover the entire body. - ANSWER -False What linear, double-stranded, enveloped DNA virus is the first virus (as of Oct. 26, 1977) to be officially declared eradicated? - ANSWER -Smallpox also known as variola virus