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MIBO 3500 EXAM 1- MICROBIOLOGY QUESTIONS

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MIBO 3500 EXAM 1- MICROBIOLOGY QUESTIONS

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December 28, 2025
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2025/2026
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MIBO 3500 EXAM 1- MICROBIOLOGY QUESTIONS

What are the three primary scientific measurements used? - Answers -1. millimeters
(mm)
2. micrometers (µm)
3. nanometers (nm)

What is the relationship between the following measurements to meters?

1. millimeters (mm)
2. micrometers (µm)
3. nanometers (nm) - Answers -1. millimeters (mm) = 10^-3 m
2. micrometers (µm) = 10^-6 m
3. nanometers (nm) = 10^-9 m

What are microbes? - Answers -Microbes are organisms or acellular agents that are
too small to be seen by an unaided eye.

What is the limit of detection of an unaided human eye? - Answers -~100-200 µm

You are caring for a patient actively infected with Mycobacterium tuberculosis. Size of
contaminated respiratory droplets are 1000-5000 nm in size.

Standard surgical masks are designed to block particles larger than 100 μm. Will
wearing a standard surgical mask be an effective form of protection? - Answers -
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The simple definition of a microbe has 3 contradictions. What are these contradictions?
- Answers -1. Supersize Microbial Cells
2. Microbial Colonies
3. Viruses

What is Robert Hooke known for? - Answers -1. Built the first compound microscope.
2. Used the compound microscope to observe mold, fleas, and cork.
3. Published Micrographia
4. Coined the term "cell".

What is Antonie van Leeuwenhoek known for? - Answers -1. Build the single-lens
magnifiers.
2. First to observe single-celled microbes.
3. He called the single-celled microbes "small animals".

What is spontaneous generation? - Answers -Spontaneous generation is the idea that
living organisms are derived from non-living organisms.

, What is Francesco Redi known for? - Answers -Francesco Redi was known for testing
the theory of spontaneous generation.

He set up 3 containers, all with a piece of meat and were left out for a few days.

The 1st container was uncovered and had the presence of flies/maggots, the 2nd
container had a paper cover and did not have the presence of flies/maggots, and the
3rd container had a gauze cover and had the presence of flies/maggots.

If spontaneous generation was true, then all 3 containers should have the presence of
flies/maggots. This experiment disproves the theory of spontaneous generation.

What is Lazzaro Spallanzani known for? - Answers -Lazzaro Spallanzani was known
for testing the theory of spontaneous generation.

He had two separate flasks and both contained broth. He boiled the broth in the flask,
leaving one covered and one uncovered, and let it cool down to room temperature.

The uncovered flask was cloudy while the covered flask was clear. Cloudy broth
indicated that microbes are present.

What is Louis Pasteur known for? - Answers -Louis Pasteur was known for testing the
theory of spontaneous generation.

He used a swan-neck flask to heat up broth and allowed for the broth to cool down. The
neck design of the flask allowed for air to come in, but traps dust and microbes in the
neck. He saw that when dust is trapped in the neck, the broth was clear. But when the
neck was broken, microbes enter and turned the broth cloudy.

What is the germ theory? - Answers -The theory that many diseases are caused by
microbes.

What are the important principles of the germ theory? - Answers -1. Chain of Infection
2. Pure Culture
3. Colonies

What does the principle "Chain of Infection" mean in the germ theory? - Answers -
Transmission - meaning that microorganism is somehow transmitted to a healthy host
and causes disease.

What does the principle "Pure Culture" mean in the germ theory? - Answers -A culture
from a single parent cell - meaning scientist must determine if there was one microbe or
multiple microbes in the culture.

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