MIBO 3500L EXAM 2 UGA QUESTIONS & ANSWERS
Extremophiles - answers -microbes that can grow at harsh conditions such as boiling
mud pits or the arctic circle
Hyperthermophile - answers -survive at >70℃
Thermophile - answers -optimal growth between 45 and 70℃
Mesophile - answers -25-45℃ (human body temp)
Psychrotrophs - answers -prefers 15-30℃
Psychrophiles - answers --5-15℃
Neutrophile - answers -ph of 7
Alkaliphiles - answers ->8.5 ph
Acidophiles - answers -<5.5 ph
Halotolerant - answers -survive up to 10% solute concentration
Halophiles - answers -require high solute concentrations to grow (e.g. Marine bacteria)
Aerobic - answers -require oxygen to grow
Aerotolerant - answers -don't use oxygen but can grow in its presence
Microaerophile - answers -require small amounts of oxygen to grow
Facultative anaerobes - answers -use oxygen to grow but can grow without it
Anaerobes - answers -cannot grow in the presence of oxygen
Conditions for temp test in 3.1 - answers -incubate at 4℃, 37℃, and 50℃
Conditions for ph test in 3.1 - answers -tsbye plates adjusted to 2,6, and 10 ph
Conditions for osmotic pressure test in 3.1 - answers -tsbye plates adjusted to 0%,
10%, and 25% nacl
Conditions for oxygen availability test in 3.1 - answers -anaerobic, microaerobic, and
aerobic plates
, How does the candle jar create a microaerobic environment? - answers -once the
candle in the jar goes out, just enough oxygen remains to sustain a microaerobic
organism
Why do we want to control the growth of bacteria? - answers -they can cause damage
to food crops and livestock and cause infection
Bacteriocidal - answers -kill the target microbe
Bacteriostatic - answers -inhibit growth of the microbe without killing it
What is an autoclave and how does it work - answers
-a chamber that uses moist heat and high pressure to kill microbes. Denatures most
cellular proteins
What is incineration - answers
Extremophiles - answers -microbes that can grow at harsh conditions such as boiling
mud pits or the arctic circle
Hyperthermophile - answers -survive at >70℃
Thermophile - answers -optimal growth between 45 and 70℃
Mesophile - answers -25-45℃ (human body temp)
Psychrotrophs - answers -prefers 15-30℃
Psychrophiles - answers --5-15℃
Neutrophile - answers -ph of 7
Alkaliphiles - answers ->8.5 ph
Acidophiles - answers -<5.5 ph
Halotolerant - answers -survive up to 10% solute concentration
Halophiles - answers -require high solute concentrations to grow (e.g. Marine bacteria)
Aerobic - answers -require oxygen to grow
Aerotolerant - answers -don't use oxygen but can grow in its presence
Microaerophile - answers -require small amounts of oxygen to grow
Facultative anaerobes - answers -use oxygen to grow but can grow without it
Anaerobes - answers -cannot grow in the presence of oxygen
Conditions for temp test in 3.1 - answers -incubate at 4℃, 37℃, and 50℃
Conditions for ph test in 3.1 - answers -tsbye plates adjusted to 2,6, and 10 ph
Conditions for osmotic pressure test in 3.1 - answers -tsbye plates adjusted to 0%,
10%, and 25% nacl
Conditions for oxygen availability test in 3.1 - answers -anaerobic, microaerobic, and
aerobic plates
, How does the candle jar create a microaerobic environment? - answers -once the
candle in the jar goes out, just enough oxygen remains to sustain a microaerobic
organism
Why do we want to control the growth of bacteria? - answers -they can cause damage
to food crops and livestock and cause infection
Bacteriocidal - answers -kill the target microbe
Bacteriostatic - answers -inhibit growth of the microbe without killing it
What is an autoclave and how does it work - answers
-a chamber that uses moist heat and high pressure to kill microbes. Denatures most
cellular proteins
What is incineration - answers
