Lecture notes Unit 18 - Gene Technology

GENE TRANSFER
removingandgene from
0 a one


organism
another
transferring it to
so that the
organism
gene
is Expressed




€
( t
isolation
gere
> -1mi


a)
restriction

restriction ☒M
④
Endonuclease

endonuclease "" """
%
> <




recombinant plasmid
t

, the desired
identification of
◦

gere
◦
be isolated
gere
can

chromosome
→
cutting from a restriction
using Endonuclease
or


using transcriptase to make
single
→
reverse a

strand
of complimentary DNA
from
another
mRNA


double synthesizes
polymerase poly nucleotide
→
DNA
to
form stranded
complimentary DNA
-




chain reactors
multiplication of the (
gere Polymerase
◦




vector
Transfer of gene into
◦


organism using
a



the cells with the
Identification of
◦


using
new
gere
genetic marker which then
a is cloned



GEI-VETICTECHNOL-OGYTOO.si
)
Enzymes restriction
→
Endonuclease
→
reverse transcriptase
ligase
→



→
DNA
polymerase
2)
synthetic DNA