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AQA GCSE Combined Science Trilogy Biology 8464/1H Actual 2026 Exam Paper + Mark Scheme (Fully Merged)

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Prepare for the AQA GCSE Combined Science: Trilogy Biology Paper 1H (8464/B/1H) – 2025 Higher Tier with a comprehensive Question Paper & Mark Scheme Revision Resource. The June 2025 paper was a 70-mark, 1 hour 15 minute Higher Tier assessment covering Cell Biology, Organisation, Infection and Response, and Bioenergetics. The 2025 paper included topics such as heart and blood vessels, pacemakers, vaccination and immunity, pathogens, photosynthesis, osmosis, inverse-square calculations, plant transport, transpiration, microscopy, stem cells, anaemia, and blood-related applications. Ideal for GCSE Combined Science Higher Tier revision, self-marking, teacher-led review, and 2026 exam preparation. For marketplace accuracy, describe it as a 2025 past-paper and marking-scheme resource rather than a current or leaked 2026 examination.

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,A student places identical potato cylinders into four solutions.

Solution Initial mass / g Final mass / g
A 2.40 2.76
B 2.40 2.52
C 2.40 2.39
D 2.40 2.12

Which solution was most likely closest to the concentration inside the potato cells?

A. A
B. B
C. C
D. D

Correct answer: C

Rationale:
There is almost no change in mass in solution C. This suggests there was little or no net
movement of water, meaning the water concentration outside the cells was approximately
equal to that inside the cells.



Question 2

A microscope image of a cell has a diameter of 36 mm. The actual diameter of the cell is 12
μm.

What is the magnification?

A. ×30
B. ×300
C. ×3000
D. ×30 000

Correct answer: C

Rationale:
36 mm = 36 000 μm.

Magnification = image size ÷ actual size

36 000 ÷ 12 = 3000.

The major trap is failing to convert millimetres into micrometres.

,Question 3

A student claims:

"A bacterial cell must contain a nucleus because it contains genetic material."

Which statement is correct?

A. Correct, because all cells containing DNA have nuclei.

B. Correct, because DNA can only function inside a nucleus.

C. Incorrect, because bacterial DNA is not enclosed within a nucleus.

D. Incorrect, because bacteria do not contain DNA.

Correct answer: C

Rationale:
Bacteria are prokaryotic cells. Their genetic material is not enclosed in a nucleus. Bacteria do
contain DNA.



Question 4

A cell contains many mitochondria and a large number of ribosomes.

Which combination best explains this?

A. The cell requires large amounts of energy and synthesises many proteins.

B. The cell carries out photosynthesis and stores large quantities of water.

C. The cell requires less energy but produces large quantities of glucose.

D. The cell is specialised only for transporting oxygen.

Correct answer: A

Rationale:
Mitochondria are the site of aerobic respiration, which releases energy for cellular processes.
Ribosomes are involved in protein synthesis. A cell containing many of both structures is
likely highly active.



Question 5

A tumour develops after a mutation affects a gene controlling cell division.

, Which sequence is most accurate?

A. Mutation → cell death → uncontrolled division → tumour

B. Mutation → uncontrolled cell division → accumulation of abnormal cells → tumour

C. Mutation → increased respiration → cell specialisation → tumour

D. Mutation → osmosis → cell enlargement → tumour

Correct answer: B

Rationale:
Mutations can affect genes controlling the cell cycle. If normal controls are lost, cells may
divide uncontrollably, producing an abnormal mass of cells.



Question 6

An enzyme works at 37 °C. Its rate increases when temperature rises from 20 °C to 35 °C but
decreases sharply at 50 °C.

Which explanation is best?

A. The enzyme becomes more concentrated at 50 °C.

B. The substrate disappears because enzymes are used up.

C. The enzyme's active site changes shape at high temperature.

D. The enzyme gains additional active sites at 50 °C.

Correct answer: C

Rationale:
At temperatures above the optimum, bonds maintaining the enzyme's structure can be
disrupted. The active site changes shape and becomes less complementary to the substrate.
The enzyme is denatured.



Question 7

An enzyme has an optimum pH of 7.

Which change would most directly reduce enzyme activity?

A. Increasing substrate concentration while substrate is limiting

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