cell 201 midterm 2 Exam
Comprehensive Questions
with Verified Answers
Graded A+
1. All of the following are part of the core oligosaccharide side chain
in the ER, except Answer:
a. glucose
b. mannose
c. N-acetyl glucosamine
d. erythrose
e. both choices A and C Answer: D
2. A protein is destined to be secreted from a cell. In which of the
following organelles would you expect to find the protein first?
Select one Answer:
a. secretory vesicle
b. Golgi apparatus
c. lysosome
d. endosome Answer: B
3. Expression of a GFP fusion protein is induced in a cell and
followed through the various organelles in the secretory pathway
using microscopy. After many hours, all of the GFP protein is in the
Golgi apparatus. This suggests that the Select one Answer:
a. protein was not translated by a ribosome
b. it is likely a resident Golgi protein
c. the protein probably has a HDEL signal
,d. it is a target of the ERAD pathway Answer: B
4. Cells of the pancreas produce and release enzymes to the
digestive system after the ingestion of food. The type of secretory
mechanism associated with the pancreatic cells is
Select one Answer:
a. intracellular secretion
b. consistent secretion
c. transport secretion
d. constitutive secretion
e. regulated secretion. Answer: E
,5. What would be the most likely difference found between
proteins in the cis-Golgi compartment and those same
proteins in the trans-Golgi? Select one Answer:
a. protein length
b. cis-Golgi proteins would lack mannose
c. only trans-Golgi proteins would be glycosylated
d. the amount of glycosylation
e. be identical Answer: D
6. In a longitudinal section of a nerve cell, at the presynaptic site
you note a variety of vesicles that are anchored to the plasma
membrane. The vesicles are likely specialized for
Select one Answer:
a. regulated secretion
b. constitutive secretion
c. hyper secretion
d. zymogen secretion
e. none of the above Answer: A
7. The endoplasmic reticulum is a
site for Select one Answer:
a. potassium storage in muscle cells
b. addition of mannose-6-phosphate
c. N-linked glycosylation of polypeptides
d. drug detoxification by catalase
e. both choices B and C Answer: C
8. Using the techniques of genetic engineering, you want to alter a
cytoplasmic protein so that it accumulates within the ER of yeast
cells. To accomplish this goal, you need to ensure that the protein
contains
Select one Answer:
a. a HDEL tag
b. do nothing; all proteins go to the ER
, c. a Kozak sequence
Comprehensive Questions
with Verified Answers
Graded A+
1. All of the following are part of the core oligosaccharide side chain
in the ER, except Answer:
a. glucose
b. mannose
c. N-acetyl glucosamine
d. erythrose
e. both choices A and C Answer: D
2. A protein is destined to be secreted from a cell. In which of the
following organelles would you expect to find the protein first?
Select one Answer:
a. secretory vesicle
b. Golgi apparatus
c. lysosome
d. endosome Answer: B
3. Expression of a GFP fusion protein is induced in a cell and
followed through the various organelles in the secretory pathway
using microscopy. After many hours, all of the GFP protein is in the
Golgi apparatus. This suggests that the Select one Answer:
a. protein was not translated by a ribosome
b. it is likely a resident Golgi protein
c. the protein probably has a HDEL signal
,d. it is a target of the ERAD pathway Answer: B
4. Cells of the pancreas produce and release enzymes to the
digestive system after the ingestion of food. The type of secretory
mechanism associated with the pancreatic cells is
Select one Answer:
a. intracellular secretion
b. consistent secretion
c. transport secretion
d. constitutive secretion
e. regulated secretion. Answer: E
,5. What would be the most likely difference found between
proteins in the cis-Golgi compartment and those same
proteins in the trans-Golgi? Select one Answer:
a. protein length
b. cis-Golgi proteins would lack mannose
c. only trans-Golgi proteins would be glycosylated
d. the amount of glycosylation
e. be identical Answer: D
6. In a longitudinal section of a nerve cell, at the presynaptic site
you note a variety of vesicles that are anchored to the plasma
membrane. The vesicles are likely specialized for
Select one Answer:
a. regulated secretion
b. constitutive secretion
c. hyper secretion
d. zymogen secretion
e. none of the above Answer: A
7. The endoplasmic reticulum is a
site for Select one Answer:
a. potassium storage in muscle cells
b. addition of mannose-6-phosphate
c. N-linked glycosylation of polypeptides
d. drug detoxification by catalase
e. both choices B and C Answer: C
8. Using the techniques of genetic engineering, you want to alter a
cytoplasmic protein so that it accumulates within the ER of yeast
cells. To accomplish this goal, you need to ensure that the protein
contains
Select one Answer:
a. a HDEL tag
b. do nothing; all proteins go to the ER
, c. a Kozak sequence
