MMSC 407 All Questions With Complete Solutions
48. What is the basis of the Biuret reaction for detecting
proteins?
A. Binding of proteins to Coomassie Brilliant Blue dye
B. The reaction of proteins with iodine
C. The interaction of peptide bonds with copper ions in an
alkaline solution
D. The absorption of light by proteins at 280 nm
C. The interaction of peptide bonds with copper ions in an
alkaline solution
49. In the Biuret reaction, what color change indicates the
presence of proteins?
A. Blue to yellow
B. Green to red
C. Red to purple
D. Light blue to violet/purple
D. Light blue to violet/purple
50. The dye binding method for measuring albumin is based on
its ability to bind
to which type of dye?
A. Neutral dyes
B. Cationic dyes
C. Anionic dyes like bromcresol green (BCG) and bromcresol
purple (BCP)
D. Fluorescent dyes
,C. Anionic dyes like bromcresol green (BCG) and bromcresol
purple (BCP)
51. In which type of electrophoresis do proteins migrate to the
pH at which their
net charge is zero?
A. SDS-PAGE
B. Isoelectric focusing (IEF)
C. Two-dimensional electrophoresis
D. Agarose gel electrophoresis
B. Isoelectric focusing (IEF)
52. Which electrophoretic method combines both isoelectric
focusing and SDS-
PAGE for protein separation?
A. Western blotting
B. 2D electrophoresis (Two-dimensional electrophoresis)
C. Capillary electrophoresis
D. Pulsed-field gel electrophoresis
B. 2D electrophoresis (Two-dimensional electrophoresis)
53. Which of the following protein fractions is typically the
largest in a normal
Serum Protein Electrophoresis pattern?
A. Alpha-1 globulins
B. Gamma globulins
C. Albumin
D. Beta globulins
C. Albumin
,54. An elevated gamma region in SPE is most often associated
with:
A. Liver failure
B. Monoclonal gammopathy or chronic inflammation
C. Acute infection
D. Hyperthyroidism
B. Monoclonal gammopathy or chronic inflammation
55. In Serum Protein Electrophoresis, which protein fraction
migrates the fastest
toward the anode?
A. Gamma globulins
B. Alpha-2 globulins
C. Albumin
D. Beta globulins
C. Albumin
56. Which of the following is a potential clinical application of
SPE?
A. Diagnosis of diabetes
B. Monitoring electrolyte imbalance
C. Detecting and monitoring monoclonal gammopathies (e.g.,
multiple myeloma)
D. Identifying bacterial infections directly
C. Detecting and monitoring monoclonal gammopathies (e.g.,
multiple myeloma)
57. What does the presence of a sharp monoclonal spike (M-
spike) in the gamma
, region of an SPE indicate?
A. Acute viral infection
B. Iron-deficiency anemia
C. Multiple myeloma or other monoclonal gammopathy
D. Liver cirrhosis
C. Multiple myeloma or other monoclonal gammopathy
58. In nephrotic syndrome, which serum protein electrophoresis
finding is
commonly observed?
A. Increased albumin and gamma globulins
B. Decreased alpha-2 globulin
C. Decreased albumin with increased alpha-2 globulin
D. Increased gamma globulin
C. Decreased albumin with increased alpha-2 globulin
59. Which pattern on SPE suggests liver cirrhosis?
A. Narrow monoclonal spike in gamma region
B. Broad beta-gamma bridging
C. Elevated alpha-1 globulin only
D. Flat albumin peak with normal globulins
B. Broad beta-gamma bridging
60. An increase in the α2-globulin fraction on serum protein
electrophoresis is
most commonly associated with which condition?
A. Dehydration
B. Inflammatory disorders
48. What is the basis of the Biuret reaction for detecting
proteins?
A. Binding of proteins to Coomassie Brilliant Blue dye
B. The reaction of proteins with iodine
C. The interaction of peptide bonds with copper ions in an
alkaline solution
D. The absorption of light by proteins at 280 nm
C. The interaction of peptide bonds with copper ions in an
alkaline solution
49. In the Biuret reaction, what color change indicates the
presence of proteins?
A. Blue to yellow
B. Green to red
C. Red to purple
D. Light blue to violet/purple
D. Light blue to violet/purple
50. The dye binding method for measuring albumin is based on
its ability to bind
to which type of dye?
A. Neutral dyes
B. Cationic dyes
C. Anionic dyes like bromcresol green (BCG) and bromcresol
purple (BCP)
D. Fluorescent dyes
,C. Anionic dyes like bromcresol green (BCG) and bromcresol
purple (BCP)
51. In which type of electrophoresis do proteins migrate to the
pH at which their
net charge is zero?
A. SDS-PAGE
B. Isoelectric focusing (IEF)
C. Two-dimensional electrophoresis
D. Agarose gel electrophoresis
B. Isoelectric focusing (IEF)
52. Which electrophoretic method combines both isoelectric
focusing and SDS-
PAGE for protein separation?
A. Western blotting
B. 2D electrophoresis (Two-dimensional electrophoresis)
C. Capillary electrophoresis
D. Pulsed-field gel electrophoresis
B. 2D electrophoresis (Two-dimensional electrophoresis)
53. Which of the following protein fractions is typically the
largest in a normal
Serum Protein Electrophoresis pattern?
A. Alpha-1 globulins
B. Gamma globulins
C. Albumin
D. Beta globulins
C. Albumin
,54. An elevated gamma region in SPE is most often associated
with:
A. Liver failure
B. Monoclonal gammopathy or chronic inflammation
C. Acute infection
D. Hyperthyroidism
B. Monoclonal gammopathy or chronic inflammation
55. In Serum Protein Electrophoresis, which protein fraction
migrates the fastest
toward the anode?
A. Gamma globulins
B. Alpha-2 globulins
C. Albumin
D. Beta globulins
C. Albumin
56. Which of the following is a potential clinical application of
SPE?
A. Diagnosis of diabetes
B. Monitoring electrolyte imbalance
C. Detecting and monitoring monoclonal gammopathies (e.g.,
multiple myeloma)
D. Identifying bacterial infections directly
C. Detecting and monitoring monoclonal gammopathies (e.g.,
multiple myeloma)
57. What does the presence of a sharp monoclonal spike (M-
spike) in the gamma
, region of an SPE indicate?
A. Acute viral infection
B. Iron-deficiency anemia
C. Multiple myeloma or other monoclonal gammopathy
D. Liver cirrhosis
C. Multiple myeloma or other monoclonal gammopathy
58. In nephrotic syndrome, which serum protein electrophoresis
finding is
commonly observed?
A. Increased albumin and gamma globulins
B. Decreased alpha-2 globulin
C. Decreased albumin with increased alpha-2 globulin
D. Increased gamma globulin
C. Decreased albumin with increased alpha-2 globulin
59. Which pattern on SPE suggests liver cirrhosis?
A. Narrow monoclonal spike in gamma region
B. Broad beta-gamma bridging
C. Elevated alpha-1 globulin only
D. Flat albumin peak with normal globulins
B. Broad beta-gamma bridging
60. An increase in the α2-globulin fraction on serum protein
electrophoresis is
most commonly associated with which condition?
A. Dehydration
B. Inflammatory disorders
