Question 1:
You have access to a 10x stock of running buffer. You need to make 500ml of 1x running buffer.
How much of the 10x stock do you need?
How much water do you need?
50 ml running buffer 450 ml water
Question 2:
You have allowed some cells to attach to a glass coverslip. You would like to stain the membrane
with a dye. The dye is at a concentration of 1mg/ml. You have been told that it works well at a final
concentration of 4ug/ml diluted in PBS
You have 7 coverslips to stain and 300 ul will cover the coverslip. You need to prepare enough
mixture to cover all the coverslips.
How much mixture will you need to prepare, account for pipetting error?
How much of the stock dye will you need to use?
(7 x 300) + 100 = 2200ul
C1 x v1 = c2 x v2
1000 x ? = 4 x 2200 = 8.8 ul
Question 3:
You have purified a protein but at 9mg/ml it is too concentrated. You decide to perform a serial 1:10
dilution. Determine the final concentration of the following
Neat 9mg/ml 9000ug/ml
1:10 0.9mg/ml 900ug/ml
1:100 0.09mg/ml 90ug/ml
1:1000 0.009mg/ml 9ug/ml
Question 4:
Using your protein at 9mg/ml you perform a 1:3 standard curve. Determine the final concentration
of the following
Neat
1:3 3mg/ml
1:9 1mg/ml
1:27 0.33mg/ml
1:81 0.11mg/ml
1:243 0.037mg/ml
Question 5:
To make 400 ul of a 1:250 dilution of antibody. How much antibody do you need to add?
1.6 ul
Question 6:
You need to make the following lysis buffer
10 mM Tris-Cl (pH 8.0)
You have access to a 10x stock of running buffer. You need to make 500ml of 1x running buffer.
How much of the 10x stock do you need?
How much water do you need?
50 ml running buffer 450 ml water
Question 2:
You have allowed some cells to attach to a glass coverslip. You would like to stain the membrane
with a dye. The dye is at a concentration of 1mg/ml. You have been told that it works well at a final
concentration of 4ug/ml diluted in PBS
You have 7 coverslips to stain and 300 ul will cover the coverslip. You need to prepare enough
mixture to cover all the coverslips.
How much mixture will you need to prepare, account for pipetting error?
How much of the stock dye will you need to use?
(7 x 300) + 100 = 2200ul
C1 x v1 = c2 x v2
1000 x ? = 4 x 2200 = 8.8 ul
Question 3:
You have purified a protein but at 9mg/ml it is too concentrated. You decide to perform a serial 1:10
dilution. Determine the final concentration of the following
Neat 9mg/ml 9000ug/ml
1:10 0.9mg/ml 900ug/ml
1:100 0.09mg/ml 90ug/ml
1:1000 0.009mg/ml 9ug/ml
Question 4:
Using your protein at 9mg/ml you perform a 1:3 standard curve. Determine the final concentration
of the following
Neat
1:3 3mg/ml
1:9 1mg/ml
1:27 0.33mg/ml
1:81 0.11mg/ml
1:243 0.037mg/ml
Question 5:
To make 400 ul of a 1:250 dilution of antibody. How much antibody do you need to add?
1.6 ul
Question 6:
You need to make the following lysis buffer
10 mM Tris-Cl (pH 8.0)
