MOLECULAR DIAGNOSTICS EXAM
QUESTIONS WITH COMPLETE
ANSWERS
What is need for amplifications during a PCR in-vitro? - ANSWER-1) Enzymes like
polymerase
2) Primers
3) Master Mix
4) Need to do a series of heating and a series of cooling.
Define Molecular Diagnostics - ANSWER-Use of DNA, RNA and protein aka amino
acid, to test for specific disease
What is one of the way molecular diagnostic are use in the lab and gave an
example? - ANSWER-To speed up the identification of
1) slow growing microorganism
2) Fastidious microorganisms
3) Microorganism that wont grow on conventional media>
What are the Molecular diagnostic Principle and describle each one? - ANSWER-1)
Target sequence, which is to target the a base sequence for that a single specific
microorganism.
2) Amplification which is the replication of the target sequence that we are after.
3) Detection is use to detect that we have the right sequence after all.
Describe DNA transcription - ANSWER-The process through which DNA sequence
is enzymatically copied by RNA polymerase to produce a complemetary RNA, aka
messager RNA (mRNA)
Describe the structure of DNA. - ANSWER-1) It is a double helix
2) Sugar
3) Phosphate group
4) Base
What are the base in a nucleotide? Which one are Purine and which ones are
Pyrimidines? - ANSWER-1) Purine are Adenine, and Guaine
2) Pyrimidines are Cytosine and Thymine
What are the bond between
1) nucleotides
2) 2 strands of DNA - ANSWER-1) Phosphodiester, which are strong covalent bond
2) Hydrogen bonds which are unstable.
What does it mean by the DNA are Antiparallel nature? - ANSWER-It means that
there is one strand that read form 5' to 3' and second strand read form 3'to 5'.
QUESTIONS WITH COMPLETE
ANSWERS
What is need for amplifications during a PCR in-vitro? - ANSWER-1) Enzymes like
polymerase
2) Primers
3) Master Mix
4) Need to do a series of heating and a series of cooling.
Define Molecular Diagnostics - ANSWER-Use of DNA, RNA and protein aka amino
acid, to test for specific disease
What is one of the way molecular diagnostic are use in the lab and gave an
example? - ANSWER-To speed up the identification of
1) slow growing microorganism
2) Fastidious microorganisms
3) Microorganism that wont grow on conventional media>
What are the Molecular diagnostic Principle and describle each one? - ANSWER-1)
Target sequence, which is to target the a base sequence for that a single specific
microorganism.
2) Amplification which is the replication of the target sequence that we are after.
3) Detection is use to detect that we have the right sequence after all.
Describe DNA transcription - ANSWER-The process through which DNA sequence
is enzymatically copied by RNA polymerase to produce a complemetary RNA, aka
messager RNA (mRNA)
Describe the structure of DNA. - ANSWER-1) It is a double helix
2) Sugar
3) Phosphate group
4) Base
What are the base in a nucleotide? Which one are Purine and which ones are
Pyrimidines? - ANSWER-1) Purine are Adenine, and Guaine
2) Pyrimidines are Cytosine and Thymine
What are the bond between
1) nucleotides
2) 2 strands of DNA - ANSWER-1) Phosphodiester, which are strong covalent bond
2) Hydrogen bonds which are unstable.
What does it mean by the DNA are Antiparallel nature? - ANSWER-It means that
there is one strand that read form 5' to 3' and second strand read form 3'to 5'.
