MOLECULAR DIAGNOSTICS LAB
EXAM QUESTIONS AND ANSWERS
Aneuploidy is often caused by:
A. by the absence or addition of a chromosome
B. by the absence or addition of a amino acids
C. by the absence or addition of a nucleotide
D. none of these is correct - ANSWER-A
Which is not a source for chromosome isolation for Karyotype experiment?
A. Bone Marrow
B. Mature red blood cells
C. Amniocyte
D. Lymphocytes - ANSWER-B
Treatment of HCT116 cells with TrypLE Express is performed for 25 minutes during
enzyme treatment?
A. True
B. False - ANSWER-B
KaryoMax Colcemid solution is responsible for -----------------------------------
A. arresting cells in anaphase so the chromosomes can be separated for cytogenetic
studies
B. arresting cells in metaphase so the chromosomes can be separated for
cytogenetic studies
C. arresting cells in telophase so the chromosomes can be separated for cytogenetic
studies
D. arresting cells in prophase so the chromosomes can be separated for cytogenetic
studies - ANSWER-B
The potassium chloride solution used at 0.075M in chromosome spreading is
A. a hypotonic solution
B. a normotonic solution
C. an isotonic solution
D. a hypertonic solution - ANSWER-A
The ratio of methanol to acetic acid in the ice-cold fixative is
A. 5:1:4
B. 1:1
C. 3:3
D. 3:1 - ANSWER-D
Karyotyping is the process
A. of unpairing and ordering all the chromosomes of an organism, thus providing a
genome-wide snapshot of an individual's chromosomes.
B. of pairing and ordering all the amino acids of an two or more organisms, thus
providing a genome-wide snapshot of an individual's chromosomes.
, C. of pairing and ordering all the chromosomes of an organism, thus providing a
protein-wide snapshot of an individual's chromosomes.
D. of pairing and ordering all the chromosomes of an organism, thus providing a
genome-wide snapshot of an individual's chromosomes. - ANSWER-D
CHOSE THE BEST POSSIBLE ANSWER. Down syndrome is the presence of extra
number of chromosomes in a cell, for example a human cell having 45 or 47
chromosomes (such as on chromosome 21) instead of the usual 46. An example of
aneuploidy (or aneuploid condition) is Down Syndrome, also known as:
A. Trisomy 20
B. Trisomy 21
C. Turner syndrome
D. Trisomy 30 - ANSWER-B
All are changes observed by scientists when studying a karyotype in order to
diagnose a genetic disease or diseases, EXCEPT.
A. TRANSLOCATION
B. DELETION
C. ISOMERIZATION
D. DUPLICATION - ANSWER-C
FILL IN THE BLANK SPACE. For prenatal diagnosis of genetic disorder
___________________________ specimens are best used as the source of cells for
karyotyping.
A. CSF
B. RED CELLS
C. SERUM
D. AMNIOTIC FLUID - ANSWER-D
In order to maximize the diagnostic information obtained from a chromosome
preparation, images of the individual chromosomes are arranged into a standardized
format known as a
A. Karyotype
B. Haplotype
C. Diplotype
D. All of these are correct - ANSWER-A
Human autosomes, or non-sex chromosomes, are numbered from
A. 1 to 22, in descending order by size
B. 1 to 22, in ascending order by size
C. 1 to 22, in any order order by size
D. 1 to 22, in mixed order by size - ANSWER-A
G-banding or ___________________________________banding is a technique
used in cytogenetics to produce a visible karyotype by staining condensed
chromosomes.
A. Giemsa banding
B. Grant banding
EXAM QUESTIONS AND ANSWERS
Aneuploidy is often caused by:
A. by the absence or addition of a chromosome
B. by the absence or addition of a amino acids
C. by the absence or addition of a nucleotide
D. none of these is correct - ANSWER-A
Which is not a source for chromosome isolation for Karyotype experiment?
A. Bone Marrow
B. Mature red blood cells
C. Amniocyte
D. Lymphocytes - ANSWER-B
Treatment of HCT116 cells with TrypLE Express is performed for 25 minutes during
enzyme treatment?
A. True
B. False - ANSWER-B
KaryoMax Colcemid solution is responsible for -----------------------------------
A. arresting cells in anaphase so the chromosomes can be separated for cytogenetic
studies
B. arresting cells in metaphase so the chromosomes can be separated for
cytogenetic studies
C. arresting cells in telophase so the chromosomes can be separated for cytogenetic
studies
D. arresting cells in prophase so the chromosomes can be separated for cytogenetic
studies - ANSWER-B
The potassium chloride solution used at 0.075M in chromosome spreading is
A. a hypotonic solution
B. a normotonic solution
C. an isotonic solution
D. a hypertonic solution - ANSWER-A
The ratio of methanol to acetic acid in the ice-cold fixative is
A. 5:1:4
B. 1:1
C. 3:3
D. 3:1 - ANSWER-D
Karyotyping is the process
A. of unpairing and ordering all the chromosomes of an organism, thus providing a
genome-wide snapshot of an individual's chromosomes.
B. of pairing and ordering all the amino acids of an two or more organisms, thus
providing a genome-wide snapshot of an individual's chromosomes.
, C. of pairing and ordering all the chromosomes of an organism, thus providing a
protein-wide snapshot of an individual's chromosomes.
D. of pairing and ordering all the chromosomes of an organism, thus providing a
genome-wide snapshot of an individual's chromosomes. - ANSWER-D
CHOSE THE BEST POSSIBLE ANSWER. Down syndrome is the presence of extra
number of chromosomes in a cell, for example a human cell having 45 or 47
chromosomes (such as on chromosome 21) instead of the usual 46. An example of
aneuploidy (or aneuploid condition) is Down Syndrome, also known as:
A. Trisomy 20
B. Trisomy 21
C. Turner syndrome
D. Trisomy 30 - ANSWER-B
All are changes observed by scientists when studying a karyotype in order to
diagnose a genetic disease or diseases, EXCEPT.
A. TRANSLOCATION
B. DELETION
C. ISOMERIZATION
D. DUPLICATION - ANSWER-C
FILL IN THE BLANK SPACE. For prenatal diagnosis of genetic disorder
___________________________ specimens are best used as the source of cells for
karyotyping.
A. CSF
B. RED CELLS
C. SERUM
D. AMNIOTIC FLUID - ANSWER-D
In order to maximize the diagnostic information obtained from a chromosome
preparation, images of the individual chromosomes are arranged into a standardized
format known as a
A. Karyotype
B. Haplotype
C. Diplotype
D. All of these are correct - ANSWER-A
Human autosomes, or non-sex chromosomes, are numbered from
A. 1 to 22, in descending order by size
B. 1 to 22, in ascending order by size
C. 1 to 22, in any order order by size
D. 1 to 22, in mixed order by size - ANSWER-A
G-banding or ___________________________________banding is a technique
used in cytogenetics to produce a visible karyotype by staining condensed
chromosomes.
A. Giemsa banding
B. Grant banding
