Molecular Biology Exam questions
and answers graded A+
In a prokaryotic gene, the spacer sequence is - ANS✅✅transcribed
TBP is a general transcription factor which is associated with eukaryotic RNA polymerase -
ANS✅✅I, II and III
which is a trans-acting factor - ANS✅✅the lac repressor LacI
core histones interact with DNA because - ANS✅✅they contain lysine and arginine-rich arms
which interact with DNA
chromatin from reticulocytes which express globin genes, and from immature reticulocytes, which
do not, was isolated and partially digested with DNase
digested chromatin was separated by size on a gel
globin gene fragments were visualised using a radiolabelled probe
one fragment was seen with DNA from immature reticulocytes whereas two smaller fragments were
seen with DNA from mature reticulocytes
this is because - ANS✅✅DNA in chromatin becomes hypersensitive to nuclease digestion when the
global gene is activated
which histone modification is exclusively associated with transcriptional activation -
ANS✅✅acetylation
eukaryotic transcription factors can be reversibly modified by phosphorylation which can then
induce several changes in function and activity of the factor, such as dimerization and localisation to
the nucleus
this is because phosphorylation can - ANS✅✅cause conformational change
the primary change which leads to transcriptional activation is brought about by interaction of
oestrogen with its receptor is - ANS✅✅dimerization
mRNA capping involves all of the following modifications but - ANS✅✅NOT acetylation
and answers graded A+
In a prokaryotic gene, the spacer sequence is - ANS✅✅transcribed
TBP is a general transcription factor which is associated with eukaryotic RNA polymerase -
ANS✅✅I, II and III
which is a trans-acting factor - ANS✅✅the lac repressor LacI
core histones interact with DNA because - ANS✅✅they contain lysine and arginine-rich arms
which interact with DNA
chromatin from reticulocytes which express globin genes, and from immature reticulocytes, which
do not, was isolated and partially digested with DNase
digested chromatin was separated by size on a gel
globin gene fragments were visualised using a radiolabelled probe
one fragment was seen with DNA from immature reticulocytes whereas two smaller fragments were
seen with DNA from mature reticulocytes
this is because - ANS✅✅DNA in chromatin becomes hypersensitive to nuclease digestion when the
global gene is activated
which histone modification is exclusively associated with transcriptional activation -
ANS✅✅acetylation
eukaryotic transcription factors can be reversibly modified by phosphorylation which can then
induce several changes in function and activity of the factor, such as dimerization and localisation to
the nucleus
this is because phosphorylation can - ANS✅✅cause conformational change
the primary change which leads to transcriptional activation is brought about by interaction of
oestrogen with its receptor is - ANS✅✅dimerization
mRNA capping involves all of the following modifications but - ANS✅✅NOT acetylation
