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MICR 290 Lecture Midterm 2025 – Study Guide & Key Concepts

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Download the MICR 290 Lecture Midterm 2025 study guide PDF for Western Carolina University (WCU). Includes key concepts, detailed explanations, and practice questions to help students review microbiology lecture material and prepare effectively for the midterm exam.

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micro 290 lecture midterm\\ MICR 290
Lecture Midterm 2025 – Study Guide & Key
Concepts
structure of eukaryotes & comparison

- membrane-bound nuclei

- mitochondrion

- fungi, protozoa, algae and plants, small multicellular animals

structure of prokaryotes (bacteria) & comparison

- unicellular, lack nuclei

- much smaller than eukaryotes

- found in places of moisture

- reproduce asexually (binary fission)

- consist of bacteria & archaea

gram positive cell wall

contains a THICK layer of peptidoglycan

gram negative cell wall

contains a THIN layer of peptidoglycan

site of electron transport chain in bacteria

Electron transfers take place in the plasma membrane of bacteria and archaea

site of electron transport chain in eukaryotes

electron transport takes place in mitochondrion

site of electron transport chain in prokaryotes

electron transport takes place in the plasma/cytoplasmic membrane of bacteria and archaea

concept of slime layer in bacteria

concept of formation of biofilms

aerobic respiration

the process of producing cellular energy in the presence of oxygen

, anaerobic respiration

transfers energy from glucose to cells

fermentation

the chemical breakdown of a substance by bacteria, yeasts, or other microorganisms, typically involving
effervescence and the giving off of heat

catabolic reaction

Catabolic (exergonic): Glycolysis (cut to 2C3 & ATP comes out)

anabolic reaction

Anabolic (endergonic): DNA/protein synthesis

endergonic reaction

general structure of DNA

A-T; C-G; DOUBLE STRANDED

general structure of RNA

A-U; C-G; SINGLE STRANDED

DNA replication

DNA duplication

DNA transcription

DNA copied to mRNA

DNA translation

mRNA is transformed/translated to a protein

cidal antibiotics

"killer" actively killing

static antibiotics

"stopper" inhibiting destruction

function of restriction endonuclease in genetic engineering

biological sensors (isolates & cuts gene at section that is shown interest)

function of ligases in genetic engineering

glues the cut piece of the human gene

steps of the gram stain

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