MICR290- Lab/lecture Final Review
Flasshcards / questions\\ MICR290
Lab/Lecture Final Exam – Study Questions &
Flashcards real exam
Which of the following represents a physical method of preventing the growth of microorganisms?
heat, cold, filtration, radiation, desiccation (removing water & moisture)
3. Which method will destroy bacterial endospores?
Autoclaving
4. What is an antibiotic?
is a type of medication used to treat bacterial infections. It works by either killing bacteria (bactericidal)
or inhibiting their growth and reproduction (bacteriostatic)
5. Which of the following methods is most suitable for disinfection of a drinking water sample
chlorination
What is the purpose of the Kirby-Bauer method?
Use of the Kirby-Bauer method, AKA disk diffusion test, is to determine the sensitivity or resistance of
bacterial strains to various antibiotics. This method helps guide the selection of appropriate antibiotics
for treating infections. In this method Zones of inhibition (clear areas where bacteria do not grow)
around the disks are measured on an agar
Epidemiologically, a disease such as Malaria is present in some regions of the world is _____
an endemic
UV on microorganisms results in which of the following lesions to the DNA?
(UV) radiation on microorganisms primarily results in the formation of thymine dimers in their DNA.
Thymine dimers are lesions where two adjacent thymine bases bond together, which disrupts the DNA
structure and inhibits replication and transcription
,8. Your patient suffers from a blood infection; after isolation, you run a Kirby-Bauer test to determine
the appropriate antibiotic for the treatment. See the results and select which is more appropriate.
1. Ciprofloxacin:
Susceptible (S): ≥ 21 mm
Intermediate (I): 16-20 mm
Resistant (R): ≤ 15 mm
2. Amoxicillin:
Susceptible (S): ≥ 18 mm
Intermediate (I): 14-17 mm
Resistant (R): ≤ 13 mm
3. Vancomycin:
Susceptible (S): ≥ 17 mm
Intermediate (I): 14-16 mm
Resistant (R): ≤ 13 mm
4. Gentamicin:
Susceptible (S): ≥ 19 mm
, Intermediate (I): 15-18 mm
Resistant (R): ≤ 14 mm
Large Zone of Inhibition:Indicates that the bacteria are susceptible to the antibiotic.Antibiotic example:
If you see a large zone around the Ciprofloxacin disk, it suggests that Ciprofloxacin is effective against
the bacteria.
Intermediate Zone of Inhibition:Indicates that the bacteria have intermediate susceptibility to the
antibiotic, which may require higher doses or combination therapy.Antibiotic example: An intermediate
zone around the Erythromycin disk might suggest limited effectiveness, possibly requiring alternative
treatment.
No Zone of Inhibition:Indicates that the bacteria are resistant to the antibiotic.Antibiotic example: No
zone around the Penicillin disk indicates that Penicillin is not effective against the bacteria.
. Which microorganism produces penicillin?
Penicillium chrysogenum and Penicillium nalgiovense
11. Variables in food safety that can promote food contamination.
Temperature control
cross-contamination
sanitation & hygiene
time control
pest control
food source
Flasshcards / questions\\ MICR290
Lab/Lecture Final Exam – Study Questions &
Flashcards real exam
Which of the following represents a physical method of preventing the growth of microorganisms?
heat, cold, filtration, radiation, desiccation (removing water & moisture)
3. Which method will destroy bacterial endospores?
Autoclaving
4. What is an antibiotic?
is a type of medication used to treat bacterial infections. It works by either killing bacteria (bactericidal)
or inhibiting their growth and reproduction (bacteriostatic)
5. Which of the following methods is most suitable for disinfection of a drinking water sample
chlorination
What is the purpose of the Kirby-Bauer method?
Use of the Kirby-Bauer method, AKA disk diffusion test, is to determine the sensitivity or resistance of
bacterial strains to various antibiotics. This method helps guide the selection of appropriate antibiotics
for treating infections. In this method Zones of inhibition (clear areas where bacteria do not grow)
around the disks are measured on an agar
Epidemiologically, a disease such as Malaria is present in some regions of the world is _____
an endemic
UV on microorganisms results in which of the following lesions to the DNA?
(UV) radiation on microorganisms primarily results in the formation of thymine dimers in their DNA.
Thymine dimers are lesions where two adjacent thymine bases bond together, which disrupts the DNA
structure and inhibits replication and transcription
,8. Your patient suffers from a blood infection; after isolation, you run a Kirby-Bauer test to determine
the appropriate antibiotic for the treatment. See the results and select which is more appropriate.
1. Ciprofloxacin:
Susceptible (S): ≥ 21 mm
Intermediate (I): 16-20 mm
Resistant (R): ≤ 15 mm
2. Amoxicillin:
Susceptible (S): ≥ 18 mm
Intermediate (I): 14-17 mm
Resistant (R): ≤ 13 mm
3. Vancomycin:
Susceptible (S): ≥ 17 mm
Intermediate (I): 14-16 mm
Resistant (R): ≤ 13 mm
4. Gentamicin:
Susceptible (S): ≥ 19 mm
, Intermediate (I): 15-18 mm
Resistant (R): ≤ 14 mm
Large Zone of Inhibition:Indicates that the bacteria are susceptible to the antibiotic.Antibiotic example:
If you see a large zone around the Ciprofloxacin disk, it suggests that Ciprofloxacin is effective against
the bacteria.
Intermediate Zone of Inhibition:Indicates that the bacteria have intermediate susceptibility to the
antibiotic, which may require higher doses or combination therapy.Antibiotic example: An intermediate
zone around the Erythromycin disk might suggest limited effectiveness, possibly requiring alternative
treatment.
No Zone of Inhibition:Indicates that the bacteria are resistant to the antibiotic.Antibiotic example: No
zone around the Penicillin disk indicates that Penicillin is not effective against the bacteria.
. Which microorganism produces penicillin?
Penicillium chrysogenum and Penicillium nalgiovense
11. Variables in food safety that can promote food contamination.
Temperature control
cross-contamination
sanitation & hygiene
time control
pest control
food source
