BIOL 4003 Module 11 Quiz well
answered already passed
During PCR, what is the order of events in a single cycle?
A. Annealing of the primers, denaturation of the DNA, synthesis of complementary strands.
B. Annealing of the primers, synthesis of complementary strands, denaturation of the DNA.
C. Denaturation of the DNA, synthesis of complementary strands, annealing the primers.
D. Denaturation of the DNA, annealing of the primers, synthesis of complementary strands. -
correct answer ✔✔ D
Why is Taq polymerase used in PCR as opposed to DNA polymerase from another source such as
E. Coli?
A. Taq polymerase makes DNA faster.
B. Taq polymerase is resistant to heat denaturation.
C. Taq polymerase is less likely to make errors.
D. All of the above would be reasons for using Taq polymerase. - correct answer ✔✔ B
, During dideoxy sequencing, dideoxynucleotides
A. Are incorporated into DNA strands more easily than deoxynucleotides.
B. Are incorporated into DNA strands but prevent further strand growth.
C. Cannot be incorporated into newly made DNA strands.
D. Can only be incorporated into newly made DNA strands via Taq polymerase. - correct answer
✔✔ B
As described in your textbook, one way to determine if a segment of chromosomal DNA has
been inserted into a vector is to put XGal in the growth media. Following overnight growth,
A. Blue colonies contain a vector with an inserted fragment of chromosomal DNA; the lacZ gene
has been inactivated.
B. White colonies contain a vector with an inserted fragment of chromosomal DNA; the lacZ
gene has been inactivated.
C. Blue colonies contain a vector with an inserted fragment of chromosomal DNA; the lacZ gene
has not been inactivated.
D. White colonies contain a vector with an inserted fragment of chromosomal DNA; the lacZ
gene has not been inactivated. - correct answer ✔✔ B
In the biotechnological production of human insulin (Humulin), explain why the A and B chains
are linked to B-galactosidase?
answered already passed
During PCR, what is the order of events in a single cycle?
A. Annealing of the primers, denaturation of the DNA, synthesis of complementary strands.
B. Annealing of the primers, synthesis of complementary strands, denaturation of the DNA.
C. Denaturation of the DNA, synthesis of complementary strands, annealing the primers.
D. Denaturation of the DNA, annealing of the primers, synthesis of complementary strands. -
correct answer ✔✔ D
Why is Taq polymerase used in PCR as opposed to DNA polymerase from another source such as
E. Coli?
A. Taq polymerase makes DNA faster.
B. Taq polymerase is resistant to heat denaturation.
C. Taq polymerase is less likely to make errors.
D. All of the above would be reasons for using Taq polymerase. - correct answer ✔✔ B
, During dideoxy sequencing, dideoxynucleotides
A. Are incorporated into DNA strands more easily than deoxynucleotides.
B. Are incorporated into DNA strands but prevent further strand growth.
C. Cannot be incorporated into newly made DNA strands.
D. Can only be incorporated into newly made DNA strands via Taq polymerase. - correct answer
✔✔ B
As described in your textbook, one way to determine if a segment of chromosomal DNA has
been inserted into a vector is to put XGal in the growth media. Following overnight growth,
A. Blue colonies contain a vector with an inserted fragment of chromosomal DNA; the lacZ gene
has been inactivated.
B. White colonies contain a vector with an inserted fragment of chromosomal DNA; the lacZ
gene has been inactivated.
C. Blue colonies contain a vector with an inserted fragment of chromosomal DNA; the lacZ gene
has not been inactivated.
D. White colonies contain a vector with an inserted fragment of chromosomal DNA; the lacZ
gene has not been inactivated. - correct answer ✔✔ B
In the biotechnological production of human insulin (Humulin), explain why the A and B chains
are linked to B-galactosidase?
