1
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MB(ASCP) Questions and Answers (100%
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what does topoisomerase do? [ ANS: ] breaks phosphate
backbone to "un" supercoil DNA
Another name for Topoisomerase II [ ANS: ] Gyrase
Helicase [ ANS: ] Un"zips" DNA at replication fork
What makes up the primosome? [ ANS: ] 1. primase
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2. helicase
3. DNA C
Guru01 - Stuvia
NASBA stands for... [ ANS: ] nucleic acid sequence based
amplification
NASBA [ ANS: ] RNA is target
RNA-->cDNA
Reverse transcriptase (RNA-->cDNA) followed by DNA dependent
polymerase (cDNA-->RNA)
RNAse H degrades original RNA template
Evaluated gene EXPRESSION [ ANS: ] RNA
ratio for organic extraction [ ANS: ] Phenol (50): Chloroform (49):
isoamyl alcohol (1)
phenol [ ANS: ] denatures proteins, dissolves lipids
chloroform [ ANS: ] assists in dissolving remaining lipids, gives a
clear interphase between aqueous phase
Long term storage of RNA [ ANS: ] 70% EtOH at -70 C
, 2
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ratio for 28S to 18S subunits [ ANS: ] 2:1 (can see this in a gel)
A260 [ ANS: ] nucleic acids
A280 [ ANS: ] protein
A230 [ ANS: ] organic compounds, phenol
A270 [ ANS: ] phenol
optical density of ds DNA [ ANS: ] 50 ug/ml
optical density ssDNA [ ANS: ] 33 ug/ml
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optical density RNA [ ANS: ] 40 ug/ml
DNA concentration is calculated: [ ANS: ] A260 x dilution factor x
50 ug/ml
Guru01 - Stuvia
RNA concentration = [ ANS: ] A260 x dilution factor x 40 ug/ml
DNA B [ ANS: ] helicase
DNA G [ ANS: ] primase
Processivity [ ANS: ] # of dNTPs that a polymerase can incorporate
continuously without dissociating from primer template
fidelity [ ANS: ] error rate of polymerase
star activity [ ANS: ] RE : additional cleavages at imperfect
recognition sites
synthesis of DNA is in what direction: [ ANS: ] 5'-->3'
add to 3' -OH
Taq DNA polymerase [ ANS: ] thermus aquaticus
thermostable
, 3
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used in amplification
3 termination sequences [ ANS: ] UGA
UAA
UAG
Huntingtons Disease (genetic basis) [ ANS: ] Autosomal Dominant
Chr 4p16
IT-IS gene gene (Huntington Protein)
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CAG repeats
<29 = normal
29-35= borderline
Guru01 - Stuvia
35< = Huntington Disease
100% penetrance
Huntington's disease phenotype [ ANS: ] degeneration of brain
cells
uncontrolled movements
loss of intellectual faculties
emotional disturbance
Cystic Fibrosis [ ANS: ] Autosomal recessive
CFTR gene
most common inherited form of mental retardation [ ANS: ] fragile
X
Fragile X [ ANS: ] FMR 1 gene on Xq27.3
CGG repeat
For Expert help and assignment solutions, +254707240657
MB(ASCP) Questions and Answers (100%
Correct Answers) Already Graded A+
what does topoisomerase do? [ ANS: ] breaks phosphate
backbone to "un" supercoil DNA
Another name for Topoisomerase II [ ANS: ] Gyrase
Helicase [ ANS: ] Un"zips" DNA at replication fork
What makes up the primosome? [ ANS: ] 1. primase
© 2025 Assignment Expert
2. helicase
3. DNA C
Guru01 - Stuvia
NASBA stands for... [ ANS: ] nucleic acid sequence based
amplification
NASBA [ ANS: ] RNA is target
RNA-->cDNA
Reverse transcriptase (RNA-->cDNA) followed by DNA dependent
polymerase (cDNA-->RNA)
RNAse H degrades original RNA template
Evaluated gene EXPRESSION [ ANS: ] RNA
ratio for organic extraction [ ANS: ] Phenol (50): Chloroform (49):
isoamyl alcohol (1)
phenol [ ANS: ] denatures proteins, dissolves lipids
chloroform [ ANS: ] assists in dissolving remaining lipids, gives a
clear interphase between aqueous phase
Long term storage of RNA [ ANS: ] 70% EtOH at -70 C
, 2
For Expert help and assignment solutions, +254707240657
ratio for 28S to 18S subunits [ ANS: ] 2:1 (can see this in a gel)
A260 [ ANS: ] nucleic acids
A280 [ ANS: ] protein
A230 [ ANS: ] organic compounds, phenol
A270 [ ANS: ] phenol
optical density of ds DNA [ ANS: ] 50 ug/ml
optical density ssDNA [ ANS: ] 33 ug/ml
© 2025 Assignment Expert
optical density RNA [ ANS: ] 40 ug/ml
DNA concentration is calculated: [ ANS: ] A260 x dilution factor x
50 ug/ml
Guru01 - Stuvia
RNA concentration = [ ANS: ] A260 x dilution factor x 40 ug/ml
DNA B [ ANS: ] helicase
DNA G [ ANS: ] primase
Processivity [ ANS: ] # of dNTPs that a polymerase can incorporate
continuously without dissociating from primer template
fidelity [ ANS: ] error rate of polymerase
star activity [ ANS: ] RE : additional cleavages at imperfect
recognition sites
synthesis of DNA is in what direction: [ ANS: ] 5'-->3'
add to 3' -OH
Taq DNA polymerase [ ANS: ] thermus aquaticus
thermostable
, 3
For Expert help and assignment solutions, +254707240657
used in amplification
3 termination sequences [ ANS: ] UGA
UAA
UAG
Huntingtons Disease (genetic basis) [ ANS: ] Autosomal Dominant
Chr 4p16
IT-IS gene gene (Huntington Protein)
© 2025 Assignment Expert
CAG repeats
<29 = normal
29-35= borderline
Guru01 - Stuvia
35< = Huntington Disease
100% penetrance
Huntington's disease phenotype [ ANS: ] degeneration of brain
cells
uncontrolled movements
loss of intellectual faculties
emotional disturbance
Cystic Fibrosis [ ANS: ] Autosomal recessive
CFTR gene
most common inherited form of mental retardation [ ANS: ] fragile
X
Fragile X [ ANS: ] FMR 1 gene on Xq27.3
CGG repeat
