lOMoARcPSD|44532475
Lab 1 Notebook
KD01
How you want to set up your labs
Objective: to establish an organized template for keeping experimental
records, procedures, and results.
Procedure:
- this is where each step of a given protocol is recorded.
- Be sure to indicate any deviations that may occur during the
experiment.
- What chemicals do you use, what temperature, what growth
conditions, and what plates?
- If there are deviations that may occur from a standard protocol that
may be given, you write that in red. Needs to be something that stands
out, so if you don’t get the result that you would expect, it may be due
to a deviation in the protocol.
Notes: additional (helpful) information placed here
Results: a summary of the final outcome of the experiment should be
described here.
Course objectives
- Cultivation of samples (growth conditions)
o Equipment used
- Identification of samples (biochemical Assays)
o Tests available
- Evaluation of samples (microscopy)
o Visualization and recognition of key characteristics
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Basic equipment
- Cleaning
o We need to work in a sterile environment and keep everyone
healthy, and the main tool for this is an autoclave.
o Autoclave 125 C (sterile conditions)
They come in different sizes
Autoclave uses heat, pressure, and steam to sterilize the
environment
It is not just dry air
Ensure an autoclave is depressurized before use because if
you open it hot steam will come out. Very slowly open it
- Growing (how we grow the bacteria and pathogens)
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Lab 1 Notebook
KD01
How you want to set up your labs
Objective: to establish an organized template for keeping experimental
records, procedures, and results.
Procedure:
- this is where each step of a given protocol is recorded.
- Be sure to indicate any deviations that may occur during the
experiment.
- What chemicals do you use, what temperature, what growth
conditions, and what plates?
- If there are deviations that may occur from a standard protocol that
may be given, you write that in red. Needs to be something that stands
out, so if you don’t get the result that you would expect, it may be due
to a deviation in the protocol.
Notes: additional (helpful) information placed here
Results: a summary of the final outcome of the experiment should be
described here.
Course objectives
- Cultivation of samples (growth conditions)
o Equipment used
- Identification of samples (biochemical Assays)
o Tests available
- Evaluation of samples (microscopy)
o Visualization and recognition of key characteristics
----------------------------------------------------------------------------------------------------
Basic equipment
- Cleaning
o We need to work in a sterile environment and keep everyone
healthy, and the main tool for this is an autoclave.
o Autoclave 125 C (sterile conditions)
They come in different sizes
Autoclave uses heat, pressure, and steam to sterilize the
environment
It is not just dry air
Ensure an autoclave is depressurized before use because if
you open it hot steam will come out. Very slowly open it
- Growing (how we grow the bacteria and pathogens)
Downloaded by madiba South Africa stuvia ()
