BIOL 334 – Past Exam Questions and Answers
Compilation Verified and Approved.
How many ATP ‘equivalents’ are produced when aerobic mammalian skeletal
muscle cells completely oxidize 1 pyruvate molecule into 3(CO2) by the
combined reactions of the pyruvate dehydrogenase complex, the TCA cycle, and
‘oxidative phosphorylation’ (i.e., assuming ‘ox-phos’ produces 3 ATP per NADH,
and 2 ATP per FADH2)?
A. 18
B. 15
C. 13
D. 12
E. 9
B
Very few genes are induced ('turned on') during hibernation of ground squirrels.
However, the 10 to 20 different
genes whose expression is increased are crucial to the survival of hibernation.
These include genes encoding:
A. several 'antioxidant' enzymes that detoxify reactive oxygen species.
B. proteins that prevent deterioration (atrophy) of skeletal muscles during
hibernation.
Page 1 of 36
,BIOL 334 – Past Exam Questions and Answers
Compilation Verified and Approved.
C. the protein kinase that phosphorylates the pyruvate dehydrogenase complex.
D. several enzymes involved in the metabolism of storage lipids.
E. all of the above.
E
Which of the following would be expected to happen if the mitochondrial outer
membrane suddenly became more permeable?
A. Loss of the proton motive force
B. Loss of ATP gradients
C. Loss of PCr gradients
D. Loss of cytochrome c
E. Loss of water
A
Which of the following is least likelyt o promote production of acetyl-CoA?
A. High mitochondrial Ca?+
B. Fatty acid beta oxidation
C. Ketolysis
D. Pyruvate reduction E. Glycolysis
A
Page 2 of 36
,BIOL 334 – Past Exam Questions and Answers
Compilation Verified and Approved.
Which of the following changes would you NOT expect tosee when
mitochondria go from state 3 to state 4?
A. A decrease in the rate of oxygen consumption
B. A decrease in TCA activity
C. A decrease in the rate of ATP synthesis
D. A decrease in the rate of water production
E. A decrease in the proton motive force
B
Which of the following statements about metabolic efficiency is FALSE?
A. Lipid oxidation generates more ATPper gram of fuel.
B. Lipid oxidation produces less ATP per oxygen consumed.
.C The ratio of ATP produced per reducing equivalent depends on whether NADH
or FADH2 is oxidized.
D. Glycogen is the preferred metabolic fuel in migrating animals because it is
rapidly mobilized.
E. The efficiency of OXPHOS depends on the permeability of themitochondrial
inner membrane.
D
Which of the following is NOTa source of reducing equivalents?
A. Isocitrate dehydrogenase
B. Lactate dehydrogenase
Page 3 of 36
, BIOL 334 – Past Exam Questions and Answers
Compilation Verified and Approved.
C. Fatty acid oxidase
D. Pyruvate dehydrogenase
E. Malate dehydrogenase
B
Which of the following reactions is NOT reversible?
A. Creatine kinase
B. F¡Fo-ATPase
C. Malate dehydrogenase
D. Citrate synthase
E. Two of the above are not reversible
B
Which of the following is least likely to regulate the rate of OXPHOS?
A. NADH/NAD*
B. Oxygen
C. Water
D. ATP/ADP
E. Phosphate
C
Page 4 of 36
Compilation Verified and Approved.
How many ATP ‘equivalents’ are produced when aerobic mammalian skeletal
muscle cells completely oxidize 1 pyruvate molecule into 3(CO2) by the
combined reactions of the pyruvate dehydrogenase complex, the TCA cycle, and
‘oxidative phosphorylation’ (i.e., assuming ‘ox-phos’ produces 3 ATP per NADH,
and 2 ATP per FADH2)?
A. 18
B. 15
C. 13
D. 12
E. 9
B
Very few genes are induced ('turned on') during hibernation of ground squirrels.
However, the 10 to 20 different
genes whose expression is increased are crucial to the survival of hibernation.
These include genes encoding:
A. several 'antioxidant' enzymes that detoxify reactive oxygen species.
B. proteins that prevent deterioration (atrophy) of skeletal muscles during
hibernation.
Page 1 of 36
,BIOL 334 – Past Exam Questions and Answers
Compilation Verified and Approved.
C. the protein kinase that phosphorylates the pyruvate dehydrogenase complex.
D. several enzymes involved in the metabolism of storage lipids.
E. all of the above.
E
Which of the following would be expected to happen if the mitochondrial outer
membrane suddenly became more permeable?
A. Loss of the proton motive force
B. Loss of ATP gradients
C. Loss of PCr gradients
D. Loss of cytochrome c
E. Loss of water
A
Which of the following is least likelyt o promote production of acetyl-CoA?
A. High mitochondrial Ca?+
B. Fatty acid beta oxidation
C. Ketolysis
D. Pyruvate reduction E. Glycolysis
A
Page 2 of 36
,BIOL 334 – Past Exam Questions and Answers
Compilation Verified and Approved.
Which of the following changes would you NOT expect tosee when
mitochondria go from state 3 to state 4?
A. A decrease in the rate of oxygen consumption
B. A decrease in TCA activity
C. A decrease in the rate of ATP synthesis
D. A decrease in the rate of water production
E. A decrease in the proton motive force
B
Which of the following statements about metabolic efficiency is FALSE?
A. Lipid oxidation generates more ATPper gram of fuel.
B. Lipid oxidation produces less ATP per oxygen consumed.
.C The ratio of ATP produced per reducing equivalent depends on whether NADH
or FADH2 is oxidized.
D. Glycogen is the preferred metabolic fuel in migrating animals because it is
rapidly mobilized.
E. The efficiency of OXPHOS depends on the permeability of themitochondrial
inner membrane.
D
Which of the following is NOTa source of reducing equivalents?
A. Isocitrate dehydrogenase
B. Lactate dehydrogenase
Page 3 of 36
, BIOL 334 – Past Exam Questions and Answers
Compilation Verified and Approved.
C. Fatty acid oxidase
D. Pyruvate dehydrogenase
E. Malate dehydrogenase
B
Which of the following reactions is NOT reversible?
A. Creatine kinase
B. F¡Fo-ATPase
C. Malate dehydrogenase
D. Citrate synthase
E. Two of the above are not reversible
B
Which of the following is least likely to regulate the rate of OXPHOS?
A. NADH/NAD*
B. Oxygen
C. Water
D. ATP/ADP
E. Phosphate
C
Page 4 of 36
