BICSP Biochemistry Technologist
Exam
Question 1. Which of the following specimens is primarily used for
measuring serum enzymes in clinical biochemistry?
A) Whole blood
B) Serum
C) Urine
D) CSF
Answer: B
Explanation: Serum is the fluid part of blood obtained after clotting, and
it is commonly used for enzyme assays because it lacks clotting factors
that could interfere with measurements.
Question 2. In specimen collection, hemolysis can interfere with tests
by:
,BICSP Biochemistry Technologist
Exam
A) Reducing serum viscosity
B) Releasing intracellular enzymes and analytes, causing falsely elevated
levels
C) Causing lipemia
D) Increasing sample stability
Answer: B
Explanation: Hemolysis releases intracellular components, such as
potassium and enzymes, leading to falsely elevated analyte levels in
tests.
Question 3. Which anticoagulant is most suitable for plasma glucose
testing to prevent glycolysis?
A) EDTA
,BICSP Biochemistry Technologist
Exam
B) Heparin
C) Sodium fluoride
D) Citrate
Answer: C
Explanation: Sodium fluoride inhibits glycolysis, preserving glucose
levels in plasma samples for accurate measurement.
Question 4. What is the primary reason for fasting before a serum
glucose test?
A) To prevent lipemia interference
B) To standardize baseline glucose levels unaffected by recent food
intake
C) To reduce specimen clotting
, BICSP Biochemistry Technologist
Exam
D) To minimize hemolysis risk
Answer: B
Explanation: Fasting ensures that recent food intake does not influence
blood glucose levels, providing a consistent baseline for diagnosis.
Question 5. Which factor does NOT significantly affect the quality of a
blood specimen?
A) Temperature during transport
B) Time elapsed before processing
C) Patient's hydration status
D) Patient's age
Answer: D
Exam
Question 1. Which of the following specimens is primarily used for
measuring serum enzymes in clinical biochemistry?
A) Whole blood
B) Serum
C) Urine
D) CSF
Answer: B
Explanation: Serum is the fluid part of blood obtained after clotting, and
it is commonly used for enzyme assays because it lacks clotting factors
that could interfere with measurements.
Question 2. In specimen collection, hemolysis can interfere with tests
by:
,BICSP Biochemistry Technologist
Exam
A) Reducing serum viscosity
B) Releasing intracellular enzymes and analytes, causing falsely elevated
levels
C) Causing lipemia
D) Increasing sample stability
Answer: B
Explanation: Hemolysis releases intracellular components, such as
potassium and enzymes, leading to falsely elevated analyte levels in
tests.
Question 3. Which anticoagulant is most suitable for plasma glucose
testing to prevent glycolysis?
A) EDTA
,BICSP Biochemistry Technologist
Exam
B) Heparin
C) Sodium fluoride
D) Citrate
Answer: C
Explanation: Sodium fluoride inhibits glycolysis, preserving glucose
levels in plasma samples for accurate measurement.
Question 4. What is the primary reason for fasting before a serum
glucose test?
A) To prevent lipemia interference
B) To standardize baseline glucose levels unaffected by recent food
intake
C) To reduce specimen clotting
, BICSP Biochemistry Technologist
Exam
D) To minimize hemolysis risk
Answer: B
Explanation: Fasting ensures that recent food intake does not influence
blood glucose levels, providing a consistent baseline for diagnosis.
Question 5. Which factor does NOT significantly affect the quality of a
blood specimen?
A) Temperature during transport
B) Time elapsed before processing
C) Patient's hydration status
D) Patient's age
Answer: D
