ASCP MLS QUESTIONS WITH CORRECT
ANSWERS 2025-2026
Whatsfeature issmost often found with patients with a splenectomy on a Wright'ssstained blood smear? -
CORRECT ANSWER -Howell-Jolly bodies
Patients with antibody to the followingsantigen are immune tosHepatitis B: -sCORRECT ANSWER -
Surface antigen (HbSAg)
Which two of the following tests are helpful for documenting previous Streptococcal throat and skin infecti
ons: - CORRECT ANSWER -ASO titer and Anti-DNase B
In mass spectrometry, the "appearance potential" is defined as the: - CORRECT ANSWER -
Minimum energy required for the appearance of a particular fragmentsion in mass spectrum
Whatsis generally accepted as the lower threshold value for semen pH from fertile males? -
CORRECT ANSWER -7.2
Coulometry: - CORRECT ANSWER -
An automated method for measuring chloride which generates silver ions in the reaction
Erythroleukemia: - CORRECT ANSWER -
Also known as Acute Myelogenous Leukemia type M6. This type is associated with either the presence of b
oth erythroid and myeloid precursors ORsstrictly erythroid precursors.
Myelomonocytic leukemia -sCORRECT ANSWERs-
Is associated with an increase in myeloid and monocytic cell precursors, but not red blood cell precursors
Species that are Lysine (+), arginine (=) and ornithine (+): -sCORRECT ANSWER -
Enterobacter aerogenes, Edwardsiella species and most Serratia species
Lysine reaction for all Citrobacter: - CORRECT ANSWER -Negative
,Key identifying characteristic for Pantoa (Enterobacter) agglomerans: - CORRECT ANSWER -
negative reactions for the three decarboxylases commonly tested ( Lysine, arginine and ornithine)
Which organism is MOST likely responsible for septicsreactions associated with Red Blood Cell transfusions
? -sCORRECT ANSWER -
Yersina entercolitica is most likely responsible for septic reactions in transfusions of Red Blood Cells. This or
ganism is usually acquired by ingestion of contaminated food and causes mild symptoms of abdominal pai
n and diarrhea. Growth of Y. entercolitica is enhanced in iron-
rich environments such as red cell components.
Gaucher'ssdisease - CORRECT ANSWER -
The most common lysosomal storage disease caused by insufficient activity of the lysosomal enzyme gluco
cerebrosidase that leads to the deposition of glucocerebroside in cells of the macrophage-
monocyte system.
Diseases associated with dysfunctionssof polymorphonuclear neutrophils (PMNs) - CORRECT ANSWER -
Chediak-
Higashi syndrome (CH), Chronic granulomatous disease (CGD), Myeloperoxidase deficiency (MPO)
NephroticsSyndrome: - CORRECT ANSWER -
Caused by damage to the kidneys, especially the basement membrane of the glomerulus; which causes ab
normal excretion of protein and red blood cells in the urine. Fats are also present in the urine in most cases
. A foamy appearance of the urine is a key characteristic of thisscondition.
Clinical symptoms include:
Swelling
Weight gain from fluid retention
Poor appetite
High blood pressure
Key Biochemical reactions for Enterobacteriaceae: - CORRECT ANSWER -
The key biochemical reactions by which the family Enterobacteriaceae can be identified include fermentati
on of carbohydrates, reduction of nitrates to nitrites and the absence of cytochrome oxidase activity.
, ANA - CORRECT ANSWER -See 2 quiz #27
Prozone effect: - CORRECT ANSWER -
Results in a false positive reaction; issthe result of antibody excess and dilution of antibody can help preven
t its occurrence
Purpose of a bi-chromatic analyzer with dual wavelengths is: - CORRECT ANSWER -
Minimize the effect of interference
Key characteristics of Micrococcus: - CORRECT ANSWER -
Susceptible to bacitracin ("A") and resistant to furazolidone; gram positive cocci in tetras, bacteria cellssof
micrococcus luteus are larger than those of the staphylococci and can arrange in tetras
Whatsis the glomerular filtration rate for a patient with a serum creatinine of 2 mg/dL, if the urine creatinin
e was 124smg/dL and the urine volume was 2.2 L/24 hrs? - CORRECT ANSWER -95 mL/min
One method of calculatingsa glomerular filtration rate is using creatinine and urine volume to determine cr
eatinine clearance. The equation is assfollows:
Creatinine Clearance = (urine creatinine X urine flow rate) / plasma creatinine; where urine flow rate = volu
me in mL /24 hours x h/60smin)
In this case = creatinine clearance = 124 X (2200/24 x hour/60) / 2 = 94.7 or 95 ml/min
Estrogen and progesterone markers are most commonly used to provide prognostic information about: -
CORRECT ANSWER -Breast cancer
The prozone effect ( when performingsa screeningstiter) issmost likely to result in: - CORRECT ANSWER -
Prozone effect (due to antibody excess) will result in an initial false negative in spitesof the large amount of
antibody in the serum, followed by a positive result as the specimen is diluted.
Which of the following Rh antigens is found the highest frequency in the Caucasian population:
A. C
ANSWERS 2025-2026
Whatsfeature issmost often found with patients with a splenectomy on a Wright'ssstained blood smear? -
CORRECT ANSWER -Howell-Jolly bodies
Patients with antibody to the followingsantigen are immune tosHepatitis B: -sCORRECT ANSWER -
Surface antigen (HbSAg)
Which two of the following tests are helpful for documenting previous Streptococcal throat and skin infecti
ons: - CORRECT ANSWER -ASO titer and Anti-DNase B
In mass spectrometry, the "appearance potential" is defined as the: - CORRECT ANSWER -
Minimum energy required for the appearance of a particular fragmentsion in mass spectrum
Whatsis generally accepted as the lower threshold value for semen pH from fertile males? -
CORRECT ANSWER -7.2
Coulometry: - CORRECT ANSWER -
An automated method for measuring chloride which generates silver ions in the reaction
Erythroleukemia: - CORRECT ANSWER -
Also known as Acute Myelogenous Leukemia type M6. This type is associated with either the presence of b
oth erythroid and myeloid precursors ORsstrictly erythroid precursors.
Myelomonocytic leukemia -sCORRECT ANSWERs-
Is associated with an increase in myeloid and monocytic cell precursors, but not red blood cell precursors
Species that are Lysine (+), arginine (=) and ornithine (+): -sCORRECT ANSWER -
Enterobacter aerogenes, Edwardsiella species and most Serratia species
Lysine reaction for all Citrobacter: - CORRECT ANSWER -Negative
,Key identifying characteristic for Pantoa (Enterobacter) agglomerans: - CORRECT ANSWER -
negative reactions for the three decarboxylases commonly tested ( Lysine, arginine and ornithine)
Which organism is MOST likely responsible for septicsreactions associated with Red Blood Cell transfusions
? -sCORRECT ANSWER -
Yersina entercolitica is most likely responsible for septic reactions in transfusions of Red Blood Cells. This or
ganism is usually acquired by ingestion of contaminated food and causes mild symptoms of abdominal pai
n and diarrhea. Growth of Y. entercolitica is enhanced in iron-
rich environments such as red cell components.
Gaucher'ssdisease - CORRECT ANSWER -
The most common lysosomal storage disease caused by insufficient activity of the lysosomal enzyme gluco
cerebrosidase that leads to the deposition of glucocerebroside in cells of the macrophage-
monocyte system.
Diseases associated with dysfunctionssof polymorphonuclear neutrophils (PMNs) - CORRECT ANSWER -
Chediak-
Higashi syndrome (CH), Chronic granulomatous disease (CGD), Myeloperoxidase deficiency (MPO)
NephroticsSyndrome: - CORRECT ANSWER -
Caused by damage to the kidneys, especially the basement membrane of the glomerulus; which causes ab
normal excretion of protein and red blood cells in the urine. Fats are also present in the urine in most cases
. A foamy appearance of the urine is a key characteristic of thisscondition.
Clinical symptoms include:
Swelling
Weight gain from fluid retention
Poor appetite
High blood pressure
Key Biochemical reactions for Enterobacteriaceae: - CORRECT ANSWER -
The key biochemical reactions by which the family Enterobacteriaceae can be identified include fermentati
on of carbohydrates, reduction of nitrates to nitrites and the absence of cytochrome oxidase activity.
, ANA - CORRECT ANSWER -See 2 quiz #27
Prozone effect: - CORRECT ANSWER -
Results in a false positive reaction; issthe result of antibody excess and dilution of antibody can help preven
t its occurrence
Purpose of a bi-chromatic analyzer with dual wavelengths is: - CORRECT ANSWER -
Minimize the effect of interference
Key characteristics of Micrococcus: - CORRECT ANSWER -
Susceptible to bacitracin ("A") and resistant to furazolidone; gram positive cocci in tetras, bacteria cellssof
micrococcus luteus are larger than those of the staphylococci and can arrange in tetras
Whatsis the glomerular filtration rate for a patient with a serum creatinine of 2 mg/dL, if the urine creatinin
e was 124smg/dL and the urine volume was 2.2 L/24 hrs? - CORRECT ANSWER -95 mL/min
One method of calculatingsa glomerular filtration rate is using creatinine and urine volume to determine cr
eatinine clearance. The equation is assfollows:
Creatinine Clearance = (urine creatinine X urine flow rate) / plasma creatinine; where urine flow rate = volu
me in mL /24 hours x h/60smin)
In this case = creatinine clearance = 124 X (2200/24 x hour/60) / 2 = 94.7 or 95 ml/min
Estrogen and progesterone markers are most commonly used to provide prognostic information about: -
CORRECT ANSWER -Breast cancer
The prozone effect ( when performingsa screeningstiter) issmost likely to result in: - CORRECT ANSWER -
Prozone effect (due to antibody excess) will result in an initial false negative in spitesof the large amount of
antibody in the serum, followed by a positive result as the specimen is diluted.
Which of the following Rh antigens is found the highest frequency in the Caucasian population:
A. C
