Lab 1 BIOL 3201 Test Questions and Answers Already Passed Latest Update 2025-2026
What is the purpose of practicing aseptic technique? - Answers To avoid contamination of pure
cultures and prevent infection.
What are the three risks of failing to use aseptic technique? - Answers 1. Contaminating others
with microorganisms. 2. Contaminating oneself. 3. Contaminating media or microorganisms
being studied.
What should you do before placing items on the lab benchtop? - Answers Disinfect your work
area.
Name some common disinfectants used in the lab. - Answers Lysol, 70% ethanol, 10% bleach,
and quaternary ammonia.
Can disinfectants sterilize surfaces or equipment? - Answers No, disinfectants can destroy
vegetative cells and viruses but may not destroy endospores.
What is the process for sterilizing loops and needles? - Answers They must be heated in a
Bunsen burner flame until red hot.
What is the recommended cooling time for a sterilized loop or needle before use? - Answers
Fifteen to twenty seconds.
What should you avoid doing with a sterilized loop or needle? - Answers Touching it to anything
after flaming, including your gloved hand.
What is the final step after inoculating a culture with a loop or needle? - Answers Flame the loop
or needle again to destroy any remaining organisms.
What is the correct method for inoculating a culture tube? - Answers Remove the cap with your
pinky, flame the mouth, insert the loop, and then flame the mouth again before closing.
How should you inoculate a slant tube? - Answers Draw the loop up the surface of the slant in a
zig-zag pattern.
What is the proper technique for inoculating Petri plates? - Answers Streak the inoculum over
the surface of the agar after flaming and cooling the loop.
What should you do after finishing your work in the lab? - Answers Disinfect the bench to ensure
any microorganisms are destroyed.
What is the first step in the Plate to Slant Transfer experiment? - Answers Gather materials
including TSA plate of Staphylococcus aureus and an uninoculated slant tube.
What is the significance of using the pinky finger to remove the cap of a culture tube? - Answers
It helps to maintain sterility by keeping the dominant hand free for the loop.
, What happens if you blow air on a flaming loop or needle? - Answers You will contaminate the
loop or needle with microorganisms.
What is the importance of the final flaming of the loop or needle? - Answers To ensure that any
remaining organisms are destroyed before placing it back.
What is the correct position for a Petri plate during inoculation? - Answers Place the Petri plate
bottom up with the lid down on the bench.
How do you sterilize the inoculating loop? - Answers Flame it until the entire wire becomes red
hot.
What should you do after touching the loop to the TSA plate culture? - Answers Close the TSA
plate by placing the bottom back on the lid.
What technique is used to streak the slant medium in the Plate to Slant Transfer? - Answers
Draw the loop up the surface of the slant in a zig-zag pattern.
What is the incubation temperature for the slant tube after inoculation? - Answers 37°C for 24
hours.
In the Broth to Plate Transfer, what is the first step after labeling the Petri plate? - Answers
Place the Petri plate lid down on the bench.
What should you do with the broth culture before using it in the Broth to Plate Transfer? -
Answers Tighten the cap and gently shake the tube to disperse the culture.
How do you streak the Petri plate during the Broth to Plate Transfer? - Answers Streak quadrant
1, then streak into quadrant 2, and repeat for quadrants 3 and 4.
What is the purpose of flaming the loop between quadrants in the Broth to Plate Transfer? -
Answers To sterilize the loop and prevent contamination.
What is the final step after completing the streaking in the Broth to Plate Transfer? - Answers
Flame the loop again and place it back into its holder.
What is the first step in the Plate to Broth Transfer experiment? - Answers Label the broth tube
with your name, seat number, date, lab day/time, and incubation temperature.
What should you do with the TSA plate before inoculating the broth tube? - Answers Place the
TSA plate bottom up on the counter.
How do you inoculate the broth in the Plate to Broth Transfer? - Answers Insert the loop into the
liquid and twirl or shake the loop to inoculate the broth.
What should you avoid doing when inserting the loop into the broth tube? - Answers Do not
touch the loop handle to the inside walls of the broth tube.
What is the purpose of practicing aseptic technique? - Answers To avoid contamination of pure
cultures and prevent infection.
What are the three risks of failing to use aseptic technique? - Answers 1. Contaminating others
with microorganisms. 2. Contaminating oneself. 3. Contaminating media or microorganisms
being studied.
What should you do before placing items on the lab benchtop? - Answers Disinfect your work
area.
Name some common disinfectants used in the lab. - Answers Lysol, 70% ethanol, 10% bleach,
and quaternary ammonia.
Can disinfectants sterilize surfaces or equipment? - Answers No, disinfectants can destroy
vegetative cells and viruses but may not destroy endospores.
What is the process for sterilizing loops and needles? - Answers They must be heated in a
Bunsen burner flame until red hot.
What is the recommended cooling time for a sterilized loop or needle before use? - Answers
Fifteen to twenty seconds.
What should you avoid doing with a sterilized loop or needle? - Answers Touching it to anything
after flaming, including your gloved hand.
What is the final step after inoculating a culture with a loop or needle? - Answers Flame the loop
or needle again to destroy any remaining organisms.
What is the correct method for inoculating a culture tube? - Answers Remove the cap with your
pinky, flame the mouth, insert the loop, and then flame the mouth again before closing.
How should you inoculate a slant tube? - Answers Draw the loop up the surface of the slant in a
zig-zag pattern.
What is the proper technique for inoculating Petri plates? - Answers Streak the inoculum over
the surface of the agar after flaming and cooling the loop.
What should you do after finishing your work in the lab? - Answers Disinfect the bench to ensure
any microorganisms are destroyed.
What is the first step in the Plate to Slant Transfer experiment? - Answers Gather materials
including TSA plate of Staphylococcus aureus and an uninoculated slant tube.
What is the significance of using the pinky finger to remove the cap of a culture tube? - Answers
It helps to maintain sterility by keeping the dominant hand free for the loop.
, What happens if you blow air on a flaming loop or needle? - Answers You will contaminate the
loop or needle with microorganisms.
What is the importance of the final flaming of the loop or needle? - Answers To ensure that any
remaining organisms are destroyed before placing it back.
What is the correct position for a Petri plate during inoculation? - Answers Place the Petri plate
bottom up with the lid down on the bench.
How do you sterilize the inoculating loop? - Answers Flame it until the entire wire becomes red
hot.
What should you do after touching the loop to the TSA plate culture? - Answers Close the TSA
plate by placing the bottom back on the lid.
What technique is used to streak the slant medium in the Plate to Slant Transfer? - Answers
Draw the loop up the surface of the slant in a zig-zag pattern.
What is the incubation temperature for the slant tube after inoculation? - Answers 37°C for 24
hours.
In the Broth to Plate Transfer, what is the first step after labeling the Petri plate? - Answers
Place the Petri plate lid down on the bench.
What should you do with the broth culture before using it in the Broth to Plate Transfer? -
Answers Tighten the cap and gently shake the tube to disperse the culture.
How do you streak the Petri plate during the Broth to Plate Transfer? - Answers Streak quadrant
1, then streak into quadrant 2, and repeat for quadrants 3 and 4.
What is the purpose of flaming the loop between quadrants in the Broth to Plate Transfer? -
Answers To sterilize the loop and prevent contamination.
What is the final step after completing the streaking in the Broth to Plate Transfer? - Answers
Flame the loop again and place it back into its holder.
What is the first step in the Plate to Broth Transfer experiment? - Answers Label the broth tube
with your name, seat number, date, lab day/time, and incubation temperature.
What should you do with the TSA plate before inoculating the broth tube? - Answers Place the
TSA plate bottom up on the counter.
How do you inoculate the broth in the Plate to Broth Transfer? - Answers Insert the loop into the
liquid and twirl or shake the loop to inoculate the broth.
What should you avoid doing when inserting the loop into the broth tube? - Answers Do not
touch the loop handle to the inside walls of the broth tube.
