ASCP- Clinical Chemistry UPDATED ACTUAL Questions and
CORRECT Answers
1. Diurnal variation Changes in concentration of an analyte
based on the time of the day (measured at
day vs. night time)
2. What is increased in the AM? (b/c of diurnal ACTH, Cortisol, Iron
variation)
3. ACTH role adrenocorticotropic hormone- stimulates
adrenal cortex to secrete CORTISOL
4. How does stress affect chemistry values? Increase in ACTH, cortisol, and cate-
cholamines
5. Catecholamines Hormones made by adrenal glands during
stress.
Ex. dopamine, epinephrine, norepineph-
rine
6. Which analyte values are affected if delay Ammonia, lactic acid, K, Mg, LD, glucose
in processing b/c serum/plasma is not sep-
arated in time?
7. Components of Spectrophotometry (in or- Light source- monochromator- cuvette-
der) photodetector- readout
8. Fluorometry components (in order) Light source- primary monochromator- cu-
vette- secondary monochromator- detec-
tor- readout
9. When is thin-layer chromatography pri- Screening test for drugs of abuse in urine
marily used?
10. When is high-performance liquid chro- Separation of thermolabile compounds
matography (HPLC) used?
, 11. When is gas chromatography used? Separation of volatile compounds (thera-
peutic & toxic drugs)
12. what is Glucose and when does it decrease major source of cellular energy. Levels de-
crease if sample sits at RT.
13. What are the common methods to mea- Glucose oxidase & hexokinase= most com-
sure glucose? mon (hexokinase is more accurate)
14. Is cholesterol a risk factor for coronary not by itself (must be analyzed with HDL &
artery disease (CAD)? LDL)
15. HDL "good fat"- levels should be >60 mg/dL
16. LDL "bad fat"- calculated via Friedwald formu-
la
Risk factor for coronary artery disease
levels should be <100 mg/dL
17. Triglycerides Main form of lipid storage- need fasting
specimen
levels should be <150 mg/dL
18. What method is used to measure total pro- Biuret method
tein?
19. Biuret method depends on the presence of Amount of PEPTIDE BONDS
what?- (detection of total protein levels)
20. What plasma protein is the most abun- albumin (synthesized in the liver)
dant?
21. Albumin levels if dehydrated Increased
CORRECT Answers
1. Diurnal variation Changes in concentration of an analyte
based on the time of the day (measured at
day vs. night time)
2. What is increased in the AM? (b/c of diurnal ACTH, Cortisol, Iron
variation)
3. ACTH role adrenocorticotropic hormone- stimulates
adrenal cortex to secrete CORTISOL
4. How does stress affect chemistry values? Increase in ACTH, cortisol, and cate-
cholamines
5. Catecholamines Hormones made by adrenal glands during
stress.
Ex. dopamine, epinephrine, norepineph-
rine
6. Which analyte values are affected if delay Ammonia, lactic acid, K, Mg, LD, glucose
in processing b/c serum/plasma is not sep-
arated in time?
7. Components of Spectrophotometry (in or- Light source- monochromator- cuvette-
der) photodetector- readout
8. Fluorometry components (in order) Light source- primary monochromator- cu-
vette- secondary monochromator- detec-
tor- readout
9. When is thin-layer chromatography pri- Screening test for drugs of abuse in urine
marily used?
10. When is high-performance liquid chro- Separation of thermolabile compounds
matography (HPLC) used?
, 11. When is gas chromatography used? Separation of volatile compounds (thera-
peutic & toxic drugs)
12. what is Glucose and when does it decrease major source of cellular energy. Levels de-
crease if sample sits at RT.
13. What are the common methods to mea- Glucose oxidase & hexokinase= most com-
sure glucose? mon (hexokinase is more accurate)
14. Is cholesterol a risk factor for coronary not by itself (must be analyzed with HDL &
artery disease (CAD)? LDL)
15. HDL "good fat"- levels should be >60 mg/dL
16. LDL "bad fat"- calculated via Friedwald formu-
la
Risk factor for coronary artery disease
levels should be <100 mg/dL
17. Triglycerides Main form of lipid storage- need fasting
specimen
levels should be <150 mg/dL
18. What method is used to measure total pro- Biuret method
tein?
19. Biuret method depends on the presence of Amount of PEPTIDE BONDS
what?- (detection of total protein levels)
20. What plasma protein is the most abun- albumin (synthesized in the liver)
dant?
21. Albumin levels if dehydrated Increased
