Molecular Biology (Exam 1)Questions
with Verified Answers
Nucleotide - ANSWERSMonomer of DNA
Nucleoside - ANSWERSMolecule of ribose plus base
2-deoxyribose - ANSWERSsugar that has a H at the 2' C
Ribose - ANSWERSName of five carbon sugar that is found in RNA
Basepair - ANSWERSTwo nucleotides, one with a purine & one with a pyrimidine,
connected by H bonds
Antiparallel means - ANSWERSthe two polynucleotide chains/strands run in opposite
directions
What two factors are the main determination of the Tm of DNA - ANSWERSAmount of
G:C's & overall strand length
How many rings do purines & pyrimidines have? - ANSWERSPurines: 2
Pyrimidines:1
The DNA from two different species can often be distinguished by a difference in
the____. - ANSWERSRatio of A+T to G+C
What is the difference between Uracil & Thymine - ANSWERSThymine has a methyl
group
What did the X-ray diffraction patterns produced by Rosalind Franklin initially reveal
about the DNA molecule? - ANSWERSIt is a helical structure with distinct regularities
You have 340 nm of double stranded B form DNA. How many 360 degree turns are
present in the double helix? - ANSWERS100
You have 340 nm of double stranded B form DNA. How many nucleotides are present?
Be careful! - ANSWERS2000
DNA and RNA both contain phosphate and deoxyribose. (True or False) -
ANSWERSFalse
, In general, the keto form of thymine is more abundant than the enol form. (True or
False) - ANSWERSTrue
Left-Handed helical form of DNA - ANSWERSZ-DNA
DNA that results from reduced humidity around the DNA and is more tightly wound -
ANSWERSA-DNA
DNA that most closely resembles the Watson & Crick model - ANSWERSB-DNA
Term used to describe the amount of the double helix crossing over itself -
ANSWERSWrithe
Number of helical turns in a given space - ANSWERSTwist
What are the main RNA secondary structures? - ANSWERSBulge-loop, hairpin,
pseudoknot, internal loop, and double helix
A closer association between DNA and histones most likely causes___. -
ANSWERSrepression in gene expression
Which amino acids of histone N-termini can be acetylated and deacetylated? -
ANSWERSLysines
RNA can form Beta Sheets (True or False) - ANSWERSFalse
What technique is most commonly used to study the secondary structure of RNA? -
ANSWERSComputer algorithms
Microarrays involve reassembling configs (true or false) - ANSWERSfalse
Incubation of cccDNA in 37 degrees Celsius water is highly likely to change the linking
number (true or false) - ANSWERSfalse
DNA wrapped around a histone core - ANSWERSnucleosome
the study of heritable changes in gene expression caused by mechanisms other than
changes in the underlying DNA sequence - ANSWERSepigenetics
the combination of histone modifications on a given nucleosome near a gene's control
region affects the efficiency of that gene's transcription - ANSWERSthe histone code
Removes acetyl group from histones - ANSWERSHDAC
methylates histones on lysine 9 of H3 - ANSWERShistone methlytransferase
with Verified Answers
Nucleotide - ANSWERSMonomer of DNA
Nucleoside - ANSWERSMolecule of ribose plus base
2-deoxyribose - ANSWERSsugar that has a H at the 2' C
Ribose - ANSWERSName of five carbon sugar that is found in RNA
Basepair - ANSWERSTwo nucleotides, one with a purine & one with a pyrimidine,
connected by H bonds
Antiparallel means - ANSWERSthe two polynucleotide chains/strands run in opposite
directions
What two factors are the main determination of the Tm of DNA - ANSWERSAmount of
G:C's & overall strand length
How many rings do purines & pyrimidines have? - ANSWERSPurines: 2
Pyrimidines:1
The DNA from two different species can often be distinguished by a difference in
the____. - ANSWERSRatio of A+T to G+C
What is the difference between Uracil & Thymine - ANSWERSThymine has a methyl
group
What did the X-ray diffraction patterns produced by Rosalind Franklin initially reveal
about the DNA molecule? - ANSWERSIt is a helical structure with distinct regularities
You have 340 nm of double stranded B form DNA. How many 360 degree turns are
present in the double helix? - ANSWERS100
You have 340 nm of double stranded B form DNA. How many nucleotides are present?
Be careful! - ANSWERS2000
DNA and RNA both contain phosphate and deoxyribose. (True or False) -
ANSWERSFalse
, In general, the keto form of thymine is more abundant than the enol form. (True or
False) - ANSWERSTrue
Left-Handed helical form of DNA - ANSWERSZ-DNA
DNA that results from reduced humidity around the DNA and is more tightly wound -
ANSWERSA-DNA
DNA that most closely resembles the Watson & Crick model - ANSWERSB-DNA
Term used to describe the amount of the double helix crossing over itself -
ANSWERSWrithe
Number of helical turns in a given space - ANSWERSTwist
What are the main RNA secondary structures? - ANSWERSBulge-loop, hairpin,
pseudoknot, internal loop, and double helix
A closer association between DNA and histones most likely causes___. -
ANSWERSrepression in gene expression
Which amino acids of histone N-termini can be acetylated and deacetylated? -
ANSWERSLysines
RNA can form Beta Sheets (True or False) - ANSWERSFalse
What technique is most commonly used to study the secondary structure of RNA? -
ANSWERSComputer algorithms
Microarrays involve reassembling configs (true or false) - ANSWERSfalse
Incubation of cccDNA in 37 degrees Celsius water is highly likely to change the linking
number (true or false) - ANSWERSfalse
DNA wrapped around a histone core - ANSWERSnucleosome
the study of heritable changes in gene expression caused by mechanisms other than
changes in the underlying DNA sequence - ANSWERSepigenetics
the combination of histone modifications on a given nucleosome near a gene's control
region affects the efficiency of that gene's transcription - ANSWERSthe histone code
Removes acetyl group from histones - ANSWERSHDAC
methylates histones on lysine 9 of H3 - ANSWERShistone methlytransferase
