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BIO250 – STRAIGHT LINE MICROBIOLOGY STUDY SET FINAL EXAM QUESTIONS AND ANSWER

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BIO250 – STRAIGHT LINE MICROBIOLOGY STUDY SET FINAL EXAM QUESTIONS AND ANSWER 1. What microbial process was responsible for changing earth’s atmosphere from one without oxygen to one with oxygen? - ANSWER Oxygenic photosynthesis 2. In the nitrogen cycle, which of the following phases involves the release of nitrogen as N2? - ANSWER Denitrification 3. In the secondary phase of wastewater treatment, why is air injected into the sludge as it is stirred and recirculated? - ANSWER The bio remediator need oxygen 4. Which of the following microorganisms is used to make yogurt? - ANSWER Lactobacillus bulgaricus 5. ATP is a - ANSWER Nucleotide that releases and stores energy in the cell 6. A viral capsid is composed of - ANSWER proteins 7. which of these are NOT considered a living organism? - ANSWER Viruses 8. which of the following describes the order in which different types of RNA are involved in translation? - ANSWER Messenger RNA (mRNA), transfer RNA (RNA), Ribosomal RNA (RNA) 9. what is the bacterial identification technique that, when positive, results in the bacterium staining two contrasting colors? - ANSWER Gram staining 10. Which of the following is found In some, but not all groups of eukaryotic microorganisms? - ANSWER cell wall 11. In which instance would a researcher need to use Bergey's manual of systemic bacteriology instead of Bergey's manual of determinative bacteriology? - ANSWER to investigate genetic history of bacterial species 12. which of the following structures is found in gram-negative bacteria but is absent from gram positive bacteria? - ANSWER outer lipid membrane 13. which type of archaea is able to survive in extremely high concentrations of salt? - ANSWER halophiles 14. which of the following is true of prokaryotes, but not true of eukaryotes? - ANSWER they have cell walls made of peptidoglycan 15. which of the following is the role of ribosomes? - ANSWER provide a site for protein synthesis 16. which of the following are theorized to be the ancestors of chloroplasts? - ANSWER cyanobacteria 17. which eukaryotic microorganisms are photoautotrophs? - ANSWER algae 18. describe the genetic material that is contained within a virus. - ANSWER RNA or DNA, not both 19. in the life cycle of an animal virus, what occurs during adsorption? - ANSWER the virus attaches to the receptor sites outside the host cell 20. which of the following viral enzymes converts viral RNA into DNA? - ANSWER reverse transcriptase 21. a bacteriophage is what type of microorganism? - ANSWER virus 22. what is the key function of a microbe's cell membrane, also known as the cytoplasmic membrane? - ANSWER it transports nutrients in and wastes out 23. the outer covering of a virus is called the - ANSWER capsid 24. which bacterial cell structure helps locomotion - ANSWER flagellum 25. what organism relies only on inorganic minerals without sun for its nutrition? - ANSWER Litho autotroph 26. what must a microbe do in active transport to absorb nutrients? - ANSWER expend energy 27. why is phosphorus an important nutrient for bacteria? - ANSWER it is a component of nucleic acids 28. during which cycle of batch culturing are growing cells most vulnerable to a change in heat? - ANSWER rapid growth 29. what is the main process by which bacterial cells reproduce? - ANSWER binary fission 30. Appendages possessed by some bacteria that aid in conjugation are called which of the following? - ANSWER pili 31. which of the following processes result in synthesis of cell molecules and structures? - ANSWER anabolism 32. how is it possible for green surfer bacteria that live on the ocean floor - where no sunligh

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CWEA CSM GRADE 1 AND 2 -
PRACTICE TEST WITH CORRECT
ANSWERS 2025-2026
1. When working with confined spaces it is critical to remember that all confined spaces are to be
considered "permit required" confined spaces until? - ANSWER Pre entry checks show
otherwise



2. You are working inside a pump station with a crew performing some technical and hazardous
maintenance. Suddenly an accident occurs that causes severe injuries to one operator. What do
you do first? - ANSWER Send for help and check and /or restore breathing



3. Based on new best practices of the common ground alliance for marking underground utilities,
the inquiry identification number for an underground service alert (USA) is viable for: - ANSWER
28 days

4. You should never attempt to install, troubleshoot, maintain, repair or replace electrical
equipment panels, controls, wiring or circuits unless: - ANSWER you know what you are doing,
are qualified, and are authorized.



5. Before starting a maintenance operation in an easement located in a backyard of a residence
you should? - ANSWER Contact the resident, and politely ask for permission to enter the
backyard.




or a nighttime Short Term Stationary Temporary Traffic Control Zone setup on low speed/low
volume street, the minimum height of the traffic cones used shall be? - ANSWER 28"

6.

Biological activity in long, sluggish-flow, flat grade sewer lines will likely cause: - ANSWER Odors

,7. According to the State Water Resources Control Board Order No. 2006-003-DWQ, each Enrollee
must develop and implement a system-specific: - ANSWER SSMP



8. What would cause a pump to deliver less than its expected rate of discharge? - ANSWER
Clogged impeller.



9. Lamping is a technique used to? - ANSWER Determine if a sewer line is straight and clear of
obstructions
10.
11. The proper testing sequence for atmospheres in confined spaces is? - ANSWER Oxygen content,
combustible gases and toxic gases



12. To find proper protective gear before application of a product, you would? - ANSWER Look at
the material safety data sheet (MSDS)




13. strength is greater than .05 TSF but less than 1.5 TSF or has angular gravel, you have what type
of soil? - ANSWER B



14. Employees whom in the course of their job, maybe required to wear respirators must be
certified medically fit. - ANSWER Annually



15. Flatter sewer line grades may cause added sewer maintenance expense and order nuisance. The
problem is most likely caused by? - ANSWER A decrease in velocity allowing gases to be
released from the wastewater



16. What are some types of sewer inspections? - ANSWER CCTV, Digital scanning, Zoom Cameras,
Nozzle-based Cameras, Sonar, Electro Scanning



17. It is unfortunate that in the wastewater industry most of our injuries are to what part of the
body? - ANSWER Head and Back



18. Legally Responsible Officials are required to report category 1 sanitary sewer overflows: -
ANSWER Within 2 hrs.

, 19. In selecting pipe material for maximum service life which factor is more important? - ANSWER
Life expectancy of pipe



20. What items would you consider when selecting a solution to clear a stoppage in a sewer? -
ANSWER Cause of stoppage




21. After completing a temporary traffic control setup for your work zone it is a good practice to: -
ANSWER Observe how effective your TTC setup is working to control traffic and make the
necessary changes to it if necessary.



22. One method of televising through flooded low spots or a dip in a sewer line is to? - ANSWER
Use a high velocity cleaner to push water clear of dip



23. The State Wide General Waste Discharge Requirements affect which of the following: -
ANSWER All owners of operations of sanitary sewer systems greater than 1-mile in length



24. In the wastewater industry we find confined spaces everywhere. What would make a routine
confined space turn into a "permit required" confined space? - ANSWER Any work that may
change the air quality




25. What items would you consider when selecting a solution to clear a stoppage in a sewer? -
ANSWER Cause of stoppage



26. In order to accurately estimate the flow of a sanitary sewer overflow (SSO) you must have: -
ANSWER Spill start time, spill end time and spill rate



27. Providing a plan and schedule to properly manage, operate and maintain all parts of the sanitary
sewer system is the goal of a: - ANSWER A sewer system management plan (SSMP)

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