QIHC (questions well answered) A+
graded
Antigen may be compromised by - correct answer ✔✔Drug therapies, prolonged fixation, time
and storage conditions, improper handling of the specimen
Hybridoma - correct answer ✔✔Cell that is the result of fusion of a tumor cell (myeloma cell)
with a normal cell
monoclonal antibodies can be raised as - correct answer ✔✔Ascites or tissue culture
supernatant
Specific staining property and very low incidence of false positive staining antibody - correct
answer ✔✔Monoclonal
Polyclonal antibodies are generally - correct answer ✔✔IgG
Polyclonal antibodies are pooled from a number of hosts who have been immunized against the
same antigen. How are they purified? - correct answer ✔✔salts and ion exchange
chromatography.
Affinity purification or isolation is done by immunoaffinity chromatography adsorbing and
eluding these contaminating proteins
Other kinds of antibodies - correct answer ✔✔Lectins and chimeras
This is any of several plant proteins that bind to specific carbohydrate groups on proteins or on
cell membranes and are used in the lab to isolate glycoproteins, to stimulate proliferation of
lymphocytes and to agglutinate RBCs - correct answer ✔✔Lectin
, Humanized antibodies - correct answer ✔✔Chimera
Purposed of antibody labeling - correct answer ✔✔Reduce background staining, cross-
reactivity, to save time performing multiple steps
Commonly used labels for antibodies - correct answer ✔✔Polymer, Biotin, HRP, Alk PHs,
flurophores
A monoclonal antibody can lead to a negative result, why? - correct answer ✔✔The specific
epitope was not available for binding with an antibody
This antibody is more sensitive but less specific - correct answer ✔✔Polyclonal
Type of pretreatments - correct answer ✔✔Proteolytic enzyme digestion
Antigen unmasking techniques (AR/HIER)
Too much pretreatment results in - correct answer ✔✔False positive
Too little pretreatment - correct answer ✔✔No staining at all
What breaks up proteins into smaller segments - correct answer ✔✔Proteolytic enzymes
Examples of Proteolytic enzymes - correct answer ✔✔Trypsin, pepsin, ficin, pronase, protease
Which enzyme solution isn't stable for more than a few hours. So needs to be made fresh
before use - correct answer ✔✔Trypsin
graded
Antigen may be compromised by - correct answer ✔✔Drug therapies, prolonged fixation, time
and storage conditions, improper handling of the specimen
Hybridoma - correct answer ✔✔Cell that is the result of fusion of a tumor cell (myeloma cell)
with a normal cell
monoclonal antibodies can be raised as - correct answer ✔✔Ascites or tissue culture
supernatant
Specific staining property and very low incidence of false positive staining antibody - correct
answer ✔✔Monoclonal
Polyclonal antibodies are generally - correct answer ✔✔IgG
Polyclonal antibodies are pooled from a number of hosts who have been immunized against the
same antigen. How are they purified? - correct answer ✔✔salts and ion exchange
chromatography.
Affinity purification or isolation is done by immunoaffinity chromatography adsorbing and
eluding these contaminating proteins
Other kinds of antibodies - correct answer ✔✔Lectins and chimeras
This is any of several plant proteins that bind to specific carbohydrate groups on proteins or on
cell membranes and are used in the lab to isolate glycoproteins, to stimulate proliferation of
lymphocytes and to agglutinate RBCs - correct answer ✔✔Lectin
, Humanized antibodies - correct answer ✔✔Chimera
Purposed of antibody labeling - correct answer ✔✔Reduce background staining, cross-
reactivity, to save time performing multiple steps
Commonly used labels for antibodies - correct answer ✔✔Polymer, Biotin, HRP, Alk PHs,
flurophores
A monoclonal antibody can lead to a negative result, why? - correct answer ✔✔The specific
epitope was not available for binding with an antibody
This antibody is more sensitive but less specific - correct answer ✔✔Polyclonal
Type of pretreatments - correct answer ✔✔Proteolytic enzyme digestion
Antigen unmasking techniques (AR/HIER)
Too much pretreatment results in - correct answer ✔✔False positive
Too little pretreatment - correct answer ✔✔No staining at all
What breaks up proteins into smaller segments - correct answer ✔✔Proteolytic enzymes
Examples of Proteolytic enzymes - correct answer ✔✔Trypsin, pepsin, ficin, pronase, protease
Which enzyme solution isn't stable for more than a few hours. So needs to be made fresh
before use - correct answer ✔✔Trypsin
