ASCP SCYM Study 2025/2026 Exam
Questions with 100% Correct Answers |
Latest Update
Sample Processing - 🧠ANSWER ✔✔Optimized antibody concentration
Defined range of cell #
Define sample handling, time and temperature
Define acceptable time of delay for acquisition
CD2 - 🧠ANSWER ✔✔T cells: NK cells
CD3 - 🧠ANSWER ✔✔T cells
CD4 - 🧠ANSWER ✔✔T helper cells, monocytes (dimly expressed)
CD5 - 🧠ANSWER ✔✔T cells; Some B Cells
CD8 - 🧠ANSWER ✔✔T cytotoxic cells, macrophages
,CD10 - 🧠ANSWER ✔✔Immature T, B Cells, PMN's
CD13 - 🧠ANSWER ✔✔Monocytes, PMN's
CD14 - 🧠ANSWER ✔✔Monocytes, PMN's (dimly expressed)
CD16 - 🧠ANSWER ✔✔NK cells, PMN's, monocytes
CD19 - 🧠ANSWER ✔✔B Cells
CD20 - 🧠ANSWER ✔✔B Cells
CD25 - 🧠ANSWER ✔✔Activated T Cells
CD33 - 🧠ANSWER ✔✔Monocytes
CD34 - 🧠ANSWER ✔✔Stem/Progenitor Cells
CD45 - 🧠ANSWER ✔✔Leukocytes
CD56 - 🧠ANSWER ✔✔NK cells, monocytes (dimly expressed)
Anti-Kappa - 🧠ANSWER ✔✔B Cells
Anti-Lambda - 🧠ANSWER ✔✔B Cells
Tdt - 🧠ANSWER ✔✔Immature Lymphocytes
,Monoclonal Antibody Staining Direct Staining - 🧠ANSWER ✔✔Rapid single
step staining
Can use multiple antibodies from the same host
Monoclonal Antibody Staining Indirect Staining - 🧠ANSWER ✔✔Secondary
amplifies single
A few labeled secondaries can detect many primaries
Clinical Applications of Flow - 🧠ANSWER ✔✔-Instrument Performance
-Integrity of the Specimen
-Quality in Processing of Specimens
-Appropriate Reagents and Controls
-Qualified Technical Staff
-Regulatory requirements from CAP, TJC, CLIA, and the FDA exist to
ensure the accurate reporting of flow cytometric results.
Lymphocytes Subset Enumeration (Immunophenotyping) - 🧠ANSWER
✔✔2 Tubes: 1- CD3/CD4/CD8/CD45
2- CD3/CD19/CD16&CD56/CD45
3
COPYRIGHT©JOSHCLAY 2025/2026. YEAR PUBLISHED 2025. COMPANY REGISTRATION NUMBER: 619652435. TERMS OF USE. PRIVACY
STATEMENT. ALL RIGHTS RESERVED
, -May include analysis of CD4 and CD8 T Cells, B Cells and NK Cells.
-Results reported as proportion (%) of total lymphocyte population.
CD4 Counts in HIV Infection - 🧠ANSWER ✔✔-Asses degree of
immunodeficiency
-Diagnose of AIDS
-Institution of Anti-Retroviral (ARV) therapy and opportunistic infection (OI)
prophylaxis
-Monitoring immune reconstitution
CD4 Immunophenotyping Panels - 🧠ANSWER ✔✔
2 color - 🧠ANSWER ✔✔Isotype Ctrl
CD45/CD14
CD3/CD4
CD3/CD8
CD3/CD19
CD3/CD16 + 56
3 Color - 🧠ANSWER ✔✔CD3/CD4/CD45
Questions with 100% Correct Answers |
Latest Update
Sample Processing - 🧠ANSWER ✔✔Optimized antibody concentration
Defined range of cell #
Define sample handling, time and temperature
Define acceptable time of delay for acquisition
CD2 - 🧠ANSWER ✔✔T cells: NK cells
CD3 - 🧠ANSWER ✔✔T cells
CD4 - 🧠ANSWER ✔✔T helper cells, monocytes (dimly expressed)
CD5 - 🧠ANSWER ✔✔T cells; Some B Cells
CD8 - 🧠ANSWER ✔✔T cytotoxic cells, macrophages
,CD10 - 🧠ANSWER ✔✔Immature T, B Cells, PMN's
CD13 - 🧠ANSWER ✔✔Monocytes, PMN's
CD14 - 🧠ANSWER ✔✔Monocytes, PMN's (dimly expressed)
CD16 - 🧠ANSWER ✔✔NK cells, PMN's, monocytes
CD19 - 🧠ANSWER ✔✔B Cells
CD20 - 🧠ANSWER ✔✔B Cells
CD25 - 🧠ANSWER ✔✔Activated T Cells
CD33 - 🧠ANSWER ✔✔Monocytes
CD34 - 🧠ANSWER ✔✔Stem/Progenitor Cells
CD45 - 🧠ANSWER ✔✔Leukocytes
CD56 - 🧠ANSWER ✔✔NK cells, monocytes (dimly expressed)
Anti-Kappa - 🧠ANSWER ✔✔B Cells
Anti-Lambda - 🧠ANSWER ✔✔B Cells
Tdt - 🧠ANSWER ✔✔Immature Lymphocytes
,Monoclonal Antibody Staining Direct Staining - 🧠ANSWER ✔✔Rapid single
step staining
Can use multiple antibodies from the same host
Monoclonal Antibody Staining Indirect Staining - 🧠ANSWER ✔✔Secondary
amplifies single
A few labeled secondaries can detect many primaries
Clinical Applications of Flow - 🧠ANSWER ✔✔-Instrument Performance
-Integrity of the Specimen
-Quality in Processing of Specimens
-Appropriate Reagents and Controls
-Qualified Technical Staff
-Regulatory requirements from CAP, TJC, CLIA, and the FDA exist to
ensure the accurate reporting of flow cytometric results.
Lymphocytes Subset Enumeration (Immunophenotyping) - 🧠ANSWER
✔✔2 Tubes: 1- CD3/CD4/CD8/CD45
2- CD3/CD19/CD16&CD56/CD45
3
COPYRIGHT©JOSHCLAY 2025/2026. YEAR PUBLISHED 2025. COMPANY REGISTRATION NUMBER: 619652435. TERMS OF USE. PRIVACY
STATEMENT. ALL RIGHTS RESERVED
, -May include analysis of CD4 and CD8 T Cells, B Cells and NK Cells.
-Results reported as proportion (%) of total lymphocyte population.
CD4 Counts in HIV Infection - 🧠ANSWER ✔✔-Asses degree of
immunodeficiency
-Diagnose of AIDS
-Institution of Anti-Retroviral (ARV) therapy and opportunistic infection (OI)
prophylaxis
-Monitoring immune reconstitution
CD4 Immunophenotyping Panels - 🧠ANSWER ✔✔
2 color - 🧠ANSWER ✔✔Isotype Ctrl
CD45/CD14
CD3/CD4
CD3/CD8
CD3/CD19
CD3/CD16 + 56
3 Color - 🧠ANSWER ✔✔CD3/CD4/CD45
