ASCP Practice Exam 1 2025/2026 Exam
Questions and Answers | 100% Solved
1. Star activity refers to - 🧠ANSWER ✔✔over-digestion
2. PCR products may be identified by the following methods EXCEPT: -
🧠ANSWER ✔✔Running on an agarose gel
3. A cosmid or cloning vector allows insertion of DNA fragments up to ____
kilobases in size. - 🧠ANSWER ✔✔40
4. Amplification infidelity is due to - 🧠ANSWER ✔✔Running on an agarose
gel
5. HPLC analysis of heteroduplexes is still being evaluated as a _______
_________ method in the clinical laboratory. - 🧠ANSWER ✔✔mutation
screening
,6. In light chain reaction production, the B-cell first attempts to generate the
kappa light chain. If unsuccessful, the cell then generates the lambda light
chain. True or False? - 🧠ANSWER ✔✔True
7. Which of the following are signal amplification methods: - 🧠ANSWER
✔✔Invader
8. Which of the following represents the order of DNA polymerase with
decreasing - 🧠ANSWER ✔✔Klenow -> Sequenase -> Taq
9. Which of the following is the most likely cause of a false positive result
on a Southern Blot for a B/T gene rearrangement? - 🧠ANSWER ✔✔Partial
digestion of genomic DNA with BamHI
10. The most common form of the DNA helix for eukaryotes is: - 🧠ANSWER
✔✔. right handed B form
11. You have just received a 9ng (stock) DNA sample and are asked to
create a 50ul dilution of this sample at 900pg. How many ul of your stock
sample do you need to create this sample? - 🧠ANSWER ✔✔5ul
12. Which polymerase synthesizes the leading strand? - 🧠ANSWER
✔✔epsilon
,13. Which of the following enzymes is best for RT PCR? - 🧠ANSWER
✔✔Reverse transcriptase
14. Which of the following techniques would be suitable for testing a
genetic condition with a large number of potential mutations within a single
gene? - 🧠ANSWER ✔✔a and b-Direct gene sequencing involves single
base resolution of sequence and therefore is best for short sequences
which contain the majority of mutations. For mutations that are not as well
clustered, mutation scanning is a better choice. FISH requires sequence-
specific probes, and therefore the sequence must be known
15. Compression on a sequencing gel is: - 🧠ANSWER ✔✔An indication of a
palindromic sequence
16. All of the following statements regarding the melting temperature of the
nucleic acid duplex are true EXCEPT: - 🧠ANSWER ✔✔Hybridization tends
to be fast at temperatures approaching the Tm of the nucleic acid duplex
17. A sample has an OD ration (260/280nm) of 0.5. This indicative of: -
🧠ANSWER ✔✔. Contamination with proteins or materials used in extraction
18. The derived linear range for a spectrophotometer is 0.0550 to 1.270.
The 260 OD absorbance reading for a sample is 1.35. Which of the
3
COPYRIGHT©JOSHCLAY 2025/2026. YEAR PUBLISHED 2025. COMPANY REGISTRATION NUMBER: 619652435. TERMS OF USE. PRIVACY
STATEMENT. ALL RIGHTS RESERVED
, following actions is the most appropriate? - 🧠ANSWER ✔✔Dilute the
sample slightly and repeat the quantitation
19. Which of the following types of DNA migrates slower than the others in
an agarose gel electrophoresis? - 🧠ANSWER ✔✔Nicked circular
20. Excess glycerol in a restriction digest reaction results in - 🧠ANSWER
✔✔diminished enzyme activity.
21. A TaqMan probes consist of a 6FAM as reporter fluorophore covalently
attached to the 5'-end of the oligonucleotide probe and a TAMRA quencher
at the 3'-end. Which of the following is the best probe considering all the
probes are listed in 5'-3' direction? - 🧠ANSWER ✔✔R-CCTAGTTACTG-Q
22. Exon 4 of the HFE gene from a patient suspected of having hereditary
hemachromatosis was amplified by PCR. The G to A mutation, frequently
found in hemachromatosis, creates an Rsa1 site in exon 4. When the PCR
products are digested with Rsa1, which of the following results would you
expect to see if the patient has the mutation? - 🧠ANSWER ✔✔the patient's
PCR product will yield extra bands upon Rsa1 digestion
23. Individuals of Ashkenazic descent are at an increased risk of all the
following disorders, EXCEPT: - 🧠ANSWER ✔✔hemochromatosis
Questions and Answers | 100% Solved
1. Star activity refers to - 🧠ANSWER ✔✔over-digestion
2. PCR products may be identified by the following methods EXCEPT: -
🧠ANSWER ✔✔Running on an agarose gel
3. A cosmid or cloning vector allows insertion of DNA fragments up to ____
kilobases in size. - 🧠ANSWER ✔✔40
4. Amplification infidelity is due to - 🧠ANSWER ✔✔Running on an agarose
gel
5. HPLC analysis of heteroduplexes is still being evaluated as a _______
_________ method in the clinical laboratory. - 🧠ANSWER ✔✔mutation
screening
,6. In light chain reaction production, the B-cell first attempts to generate the
kappa light chain. If unsuccessful, the cell then generates the lambda light
chain. True or False? - 🧠ANSWER ✔✔True
7. Which of the following are signal amplification methods: - 🧠ANSWER
✔✔Invader
8. Which of the following represents the order of DNA polymerase with
decreasing - 🧠ANSWER ✔✔Klenow -> Sequenase -> Taq
9. Which of the following is the most likely cause of a false positive result
on a Southern Blot for a B/T gene rearrangement? - 🧠ANSWER ✔✔Partial
digestion of genomic DNA with BamHI
10. The most common form of the DNA helix for eukaryotes is: - 🧠ANSWER
✔✔. right handed B form
11. You have just received a 9ng (stock) DNA sample and are asked to
create a 50ul dilution of this sample at 900pg. How many ul of your stock
sample do you need to create this sample? - 🧠ANSWER ✔✔5ul
12. Which polymerase synthesizes the leading strand? - 🧠ANSWER
✔✔epsilon
,13. Which of the following enzymes is best for RT PCR? - 🧠ANSWER
✔✔Reverse transcriptase
14. Which of the following techniques would be suitable for testing a
genetic condition with a large number of potential mutations within a single
gene? - 🧠ANSWER ✔✔a and b-Direct gene sequencing involves single
base resolution of sequence and therefore is best for short sequences
which contain the majority of mutations. For mutations that are not as well
clustered, mutation scanning is a better choice. FISH requires sequence-
specific probes, and therefore the sequence must be known
15. Compression on a sequencing gel is: - 🧠ANSWER ✔✔An indication of a
palindromic sequence
16. All of the following statements regarding the melting temperature of the
nucleic acid duplex are true EXCEPT: - 🧠ANSWER ✔✔Hybridization tends
to be fast at temperatures approaching the Tm of the nucleic acid duplex
17. A sample has an OD ration (260/280nm) of 0.5. This indicative of: -
🧠ANSWER ✔✔. Contamination with proteins or materials used in extraction
18. The derived linear range for a spectrophotometer is 0.0550 to 1.270.
The 260 OD absorbance reading for a sample is 1.35. Which of the
3
COPYRIGHT©JOSHCLAY 2025/2026. YEAR PUBLISHED 2025. COMPANY REGISTRATION NUMBER: 619652435. TERMS OF USE. PRIVACY
STATEMENT. ALL RIGHTS RESERVED
, following actions is the most appropriate? - 🧠ANSWER ✔✔Dilute the
sample slightly and repeat the quantitation
19. Which of the following types of DNA migrates slower than the others in
an agarose gel electrophoresis? - 🧠ANSWER ✔✔Nicked circular
20. Excess glycerol in a restriction digest reaction results in - 🧠ANSWER
✔✔diminished enzyme activity.
21. A TaqMan probes consist of a 6FAM as reporter fluorophore covalently
attached to the 5'-end of the oligonucleotide probe and a TAMRA quencher
at the 3'-end. Which of the following is the best probe considering all the
probes are listed in 5'-3' direction? - 🧠ANSWER ✔✔R-CCTAGTTACTG-Q
22. Exon 4 of the HFE gene from a patient suspected of having hereditary
hemachromatosis was amplified by PCR. The G to A mutation, frequently
found in hemachromatosis, creates an Rsa1 site in exon 4. When the PCR
products are digested with Rsa1, which of the following results would you
expect to see if the patient has the mutation? - 🧠ANSWER ✔✔the patient's
PCR product will yield extra bands upon Rsa1 digestion
23. Individuals of Ashkenazic descent are at an increased risk of all the
following disorders, EXCEPT: - 🧠ANSWER ✔✔hemochromatosis
