MB ASCP CONNECT 2026 EXAM
QUESTIONS AND ANSWERS
What is translocation is associated with Burkitt's Lymphoma?
a. t(18; 14)
b. t(9; 22)
c. t(8; 14)
d. t(15; 17) - Correct Answer -t(8; 14)
TIP: Burkkitt's 8 letters,
Locus PF Child Paternity Index
LOC-A1 3 2/3 2.18
LOC-B2 7/5 5 0.798
©COPYRIGHT 2025 ALL RIGHTS RESERVED 1
,LOC-C3 17/17 9/17 5.21
LOC-D4 12 12 1.37
Based on the data presented in the table above and with a prior probability (PP) of
0.5, the probability of paternity in this case is :
a. 92.5%
b. 99.5%
c. 90.5 &
d. 95.5% - Correct Answer -92.5 %
Multiply all PI together = CPI
(CPI x PP) / [CPI x P + (1 - PP)
OR ...
CPI / (1 + CPI)
©COPYRIGHT 2025 ALL RIGHTS RESERVED 2
,You have sequenced a gene and observe the following:
Reference: atgctggcacgacaggtttcccgactgg
Sequenced: atgCctggcacgacaggtttcccgactgg
The mutation observed is a:
a. Frame-shift mutation
b. Insertion
c. Silent mutation
d. Non-conservative mutation - Correct Answer -Frame-shift mutation
Which of the following is not involved in the splicing reaction?
a. 5' splice site
b. Hairpin loops
©COPYRIGHT 2025 ALL RIGHTS RESERVED 3
, c. Branch A point
d. 3' splice site - Correct Answer -Hairpin loops
Which of the following statements are characteristics of the melt curve analysis?
(hint: more than one answer)
a. At the melting point, the probe separates from the target strand and fluorescence
rapidly decreases.
b. The melting temperature of double stranded DNA depends on its base
composition and length.
c. All PCR products for a specific primer pair should have the same melting
temperature.
d. When hybridization probes are utilized, the temperature is incrementally
decreased while fluorescence is monitored. - Correct Answer -At the melting point,
the probe separates from the target strand and fluorescence rapidly decreases.
The melting temperature of double stranded DNA depends on its base composition
and length.
©COPYRIGHT 2025 ALL RIGHTS RESERVED 4
QUESTIONS AND ANSWERS
What is translocation is associated with Burkitt's Lymphoma?
a. t(18; 14)
b. t(9; 22)
c. t(8; 14)
d. t(15; 17) - Correct Answer -t(8; 14)
TIP: Burkkitt's 8 letters,
Locus PF Child Paternity Index
LOC-A1 3 2/3 2.18
LOC-B2 7/5 5 0.798
©COPYRIGHT 2025 ALL RIGHTS RESERVED 1
,LOC-C3 17/17 9/17 5.21
LOC-D4 12 12 1.37
Based on the data presented in the table above and with a prior probability (PP) of
0.5, the probability of paternity in this case is :
a. 92.5%
b. 99.5%
c. 90.5 &
d. 95.5% - Correct Answer -92.5 %
Multiply all PI together = CPI
(CPI x PP) / [CPI x P + (1 - PP)
OR ...
CPI / (1 + CPI)
©COPYRIGHT 2025 ALL RIGHTS RESERVED 2
,You have sequenced a gene and observe the following:
Reference: atgctggcacgacaggtttcccgactgg
Sequenced: atgCctggcacgacaggtttcccgactgg
The mutation observed is a:
a. Frame-shift mutation
b. Insertion
c. Silent mutation
d. Non-conservative mutation - Correct Answer -Frame-shift mutation
Which of the following is not involved in the splicing reaction?
a. 5' splice site
b. Hairpin loops
©COPYRIGHT 2025 ALL RIGHTS RESERVED 3
, c. Branch A point
d. 3' splice site - Correct Answer -Hairpin loops
Which of the following statements are characteristics of the melt curve analysis?
(hint: more than one answer)
a. At the melting point, the probe separates from the target strand and fluorescence
rapidly decreases.
b. The melting temperature of double stranded DNA depends on its base
composition and length.
c. All PCR products for a specific primer pair should have the same melting
temperature.
d. When hybridization probes are utilized, the temperature is incrementally
decreased while fluorescence is monitored. - Correct Answer -At the melting point,
the probe separates from the target strand and fluorescence rapidly decreases.
The melting temperature of double stranded DNA depends on its base composition
and length.
©COPYRIGHT 2025 ALL RIGHTS RESERVED 4