MB (ASCP) Exam 2026 Questions and
Answers
Predominant form of DNA - Correct Answer -Right-handed B
DNA and histone interaction is due to what type of bond? - Correct Answer -Ionic
What stains to the minor groove of DNA duplex? - Correct Answer -SYBR Green
What is the most abundant type of RNA? - Correct Answer -Ribosomal RNA
(rRNA)
What does RNA act as in a retrovirus? - Correct Answer -Genome and mRNA
What increases half-life of mRNA? - Correct Answer -5' Methyl Cap
What kind of DNA is most likely to be transcriptionally active? - Correct Answer -
Euchromatin
What is the most common type of eukaryotic DNA? - Correct Answer -Satellite
DNA
What specimen has the most DNA according to molecular weight? - Correct
Answer -White Blood Cells
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,What amino acids compose histones? - Correct Answer -Lysine and Arginine
Amino acid that all proteins start with: - Correct Answer -Methionine
What protects the DNA strand during replication? - Correct Answer -Single-
stranded binding protein
Leading Strand - Correct Answer -3' to 5' template
Lagging Strand - Correct Answer -5' to 3' template
What DNA strand is being copied in transcription? - Correct Answer -Antisense
Strand
First step after newly formed mRNA is made? - Correct Answer -Addition of
poly(A) tail
Every time the chromosome is copied it would become shorter if not for what
enzyme? - Correct Answer -Telomerase
What polymerase has repair activity? - Correct Answer -Beta/Epsilon
What polymerase acts on leading strand? - Correct Answer -Delta
What polymerase acts on lagging strand? - Correct Answer -Alpha
Which polymerase is used for mitochondrial replication and repair? - Correct
Answer -Gamma
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, What polymerase binds the 5' end to 3' end of dsDNA? - Correct Answer -
Topoisomerase
Which RNA polymerase is mainly for transcription? - Correct Answer -RNA Pol II
Which polymerase is involved with mRNA synthesis? - Correct Answer -RNA Pol
II
What inactivates RNAses? - Correct Answer -Diethyl pyrocarbonate (DEPC) or
bake glassware for 4 to 6 hours at 400C
What type of restriction enzyme recognizes palindromic sites? - Correct Answer -
Restriction Enzyme Type II
What type of restriction enzyme lacks methylation activity? - Correct Answer -
Restriction Enzyme Type II
What is necessary to make cDNA? - Correct Answer -Reverse transcriptase
Number of SNPs in a human genome? - Correct Answer -11 billion (10^7)
How many genes (base pairs) are in a human haploid genome? - Correct Answer -3
billion base pairs
The polymerization of acrylamide requires: - Correct Answer -Catalyst (APS or
TEMED) or Light activation
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Answers
Predominant form of DNA - Correct Answer -Right-handed B
DNA and histone interaction is due to what type of bond? - Correct Answer -Ionic
What stains to the minor groove of DNA duplex? - Correct Answer -SYBR Green
What is the most abundant type of RNA? - Correct Answer -Ribosomal RNA
(rRNA)
What does RNA act as in a retrovirus? - Correct Answer -Genome and mRNA
What increases half-life of mRNA? - Correct Answer -5' Methyl Cap
What kind of DNA is most likely to be transcriptionally active? - Correct Answer -
Euchromatin
What is the most common type of eukaryotic DNA? - Correct Answer -Satellite
DNA
What specimen has the most DNA according to molecular weight? - Correct
Answer -White Blood Cells
©COPYRIGHT 2025 ALL RIGHTS RESERVED 1
,What amino acids compose histones? - Correct Answer -Lysine and Arginine
Amino acid that all proteins start with: - Correct Answer -Methionine
What protects the DNA strand during replication? - Correct Answer -Single-
stranded binding protein
Leading Strand - Correct Answer -3' to 5' template
Lagging Strand - Correct Answer -5' to 3' template
What DNA strand is being copied in transcription? - Correct Answer -Antisense
Strand
First step after newly formed mRNA is made? - Correct Answer -Addition of
poly(A) tail
Every time the chromosome is copied it would become shorter if not for what
enzyme? - Correct Answer -Telomerase
What polymerase has repair activity? - Correct Answer -Beta/Epsilon
What polymerase acts on leading strand? - Correct Answer -Delta
What polymerase acts on lagging strand? - Correct Answer -Alpha
Which polymerase is used for mitochondrial replication and repair? - Correct
Answer -Gamma
©COPYRIGHT 2025 ALL RIGHTS RESERVED 2
, What polymerase binds the 5' end to 3' end of dsDNA? - Correct Answer -
Topoisomerase
Which RNA polymerase is mainly for transcription? - Correct Answer -RNA Pol II
Which polymerase is involved with mRNA synthesis? - Correct Answer -RNA Pol
II
What inactivates RNAses? - Correct Answer -Diethyl pyrocarbonate (DEPC) or
bake glassware for 4 to 6 hours at 400C
What type of restriction enzyme recognizes palindromic sites? - Correct Answer -
Restriction Enzyme Type II
What type of restriction enzyme lacks methylation activity? - Correct Answer -
Restriction Enzyme Type II
What is necessary to make cDNA? - Correct Answer -Reverse transcriptase
Number of SNPs in a human genome? - Correct Answer -11 billion (10^7)
How many genes (base pairs) are in a human haploid genome? - Correct Answer -3
billion base pairs
The polymerization of acrylamide requires: - Correct Answer -Catalyst (APS or
TEMED) or Light activation
©COPYRIGHT 2025 ALL RIGHTS RESERVED 3
